鸡繁殖性状近交衰退相关lncRNA的筛选

doi:10.16431/j.cnki.1671-7236.2022.06.018

摘要/Abstract

摘要： 【目的】筛选与鸡繁殖性状近交衰退相关的长链非编码RNA （long non-coding RNA，lncRNA），为深入探究lncRNA对鸡繁殖性能近交衰退调控机制提供参考。【方法】以狼山鸡高、低近交群体为试验素材，通过转录组测序技术分析狼山鸡下丘脑和卵巢中lncRNA表达情况，筛选高、低近交组间差异表达lncRNA，并对其进行顺式调控靶基因预测及功能分析。【结果】狼山鸡下丘脑和卵巢中共鉴定出4 222个lncRNAs，高、低近交组间比较发现，下丘脑中差异表达lncRNAs 35个，卵巢中差异表达lncRNAs 215个。下丘脑中差异lncRNAs中预测到顺式调控靶基因98个，这些靶基因显著富集于出生或孵化时胚胎发育终止、胚胎心导管发育、视黄酸应答等繁殖相关生物过程（P<0.05），涉及lncRNA MSTRG.9196.4、MSTRG.9195.2、MSTRG.6254.2以及相应靶基因DNAAF2和FKBP1B等胚胎发育相关基因；卵巢中差异lncRNAs预测到顺式调控靶基因414个，这些靶基因富集到卵母细胞减数分裂、MAPK、叶酸合成等信号通路，包括MSTRG.7683.1、MSTRG.13604.4、MSTRG.16570.1、MSTRG.8330.5、MSTRG.8330.4等神经内分泌调节及配子生成相关的lncRNAs。【结论】本研究在下丘脑和卵巢中筛选到了一系列鸡胚胎发育及配子生成调控相关差异lncRNAs，这些lncRNAs可作为鸡繁殖性能近交衰退候选lncRNAs，为进一步揭示鸡繁殖性能近交衰退调控机制提供线索。

关键词: 鸡; 繁殖性状; 近交衰退; 差异表达; 长链非编码RNA(lncRNA); 靶基因

Abstract: 【Objective】 This study was aimed to screen long non-coding RNA (lncRNA) related to inbreeding depression of reproduction in chickens, so as to provide reference for further exploring the regulation mechanism of lncRNA on inbreeding depression of reproduction in chickens.【Method】 The strongly and weakly inbreeding populations of Langshan chickens were used as materials, lncRNA expression in hypothalamus and ovary of Langshan chickens was analyzed by transcriptome sequencing.Differentially expressed lncRNA between strongly and weakly inbreeding populations were identified, and cis regulation target gene prediction and function analysis were performed.【Result】 A total of 4 222 lncRNAs were identified in hypothalamus and ovary of Langshan chickens.Comparing the strongly and weakly inbreeding populations, 35 and 215 differentially expressed lncRNAs were found in hypothalamus and ovary, respectively.In hypothalamus, 98 cis regulation target genes were predicted in differentially expressed lncRNAs, which were significantly enriched in reproduction-related biological processes (P<0.05), such as embryo development ending in birth or egg hatching, embryonic heart tube development and response to retinoic acid, involving MSTRG.9196.4, MSTRG.9195.2, MSTRG.6254.2 lncRNAs and their corresponding target genes DNAAF2 and FKBP1B.In ovary, 414 target genes were predicted, which were enriched in oocyte meiosis, MAPK and folic acid synthesis signaling pathways, including MSTRG.7683.1, MSTRG.13604.4, MSTRG.16570.1, MSTRG.8330.5, MSTRG.8330.4 and other lncRNAs related to neuroendocrine regulation and gametogenesis.【Conclusion】 In this study, a series of differential lncRNAs related to embryo development and gametogenesis in chickens were screened from hypothalamus and ovary.These lncRNAs could be used as candidate lncRNAs for inbreeding depression of reproduction in chickens, and provided clues for revealing the regulation mechanism of inbreeding depression in reproduction of chickens.

Key words: chickens; reproductive traits; inbreeding depression; differential expression; long non-coding RNA (lncRNA); target gene

中图分类号:

S831.2

薛倩, 李国辉, 邢伟杰, 周成浩, 张会永, 殷建玫, 朱云芬, 曹愉夏, 苏一军, 韩威. 鸡繁殖性状近交衰退相关lncRNA的筛选[J]. 中国畜牧兽医, 2022, 49(6): 2176-2185.

XUE Qian, LI Guohui, XING Weijie, ZHOU Chenghao, ZHANG Huiyong, YIN Jianmei, ZHU Yunfen, CAO Yuxia, SU Yijun, HAN Wei. Screening of lncRNA Related to Inbreeding Depression of Reproduction in Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2176-2185.

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