中国草原红牛PDK4基因多态性及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2022.06.019

中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (6): 2186-2194.doi: 10.16431/j.cnki.1671-7236.2022.06.019

中国草原红牛PDK4基因多态性及其与肉质性状的关联分析

马彦茹^1,2, 于永生², 曹阳², 秦立红², 赵玉民^2,3,4,5, 吴健^2,3,4,5

1. 吉林农业大学动物科学技术学院, 长春 130118;
2. 吉林省农业科学院, 公主岭 130033;
3. 农业农村部肉牛遗传育种重点实验室, 公主岭 136100;
4. 农业农村部东北种牛性能测定中心, 公主岭 136100;
5. 吉林省肉牛繁育及养殖技术科技创新中心, 公主岭 136100

收稿日期:2021-10-31 出版日期:2022-06-05 发布日期:2022-05-27
通讯作者: 吴健 E-mail:wujian0303@126.com
作者简介:马彦茹,E-mail:yr1519518049@163.com。
基金资助:
国家肉牛牦牛产业技术体系（CARA-37）；吉林省科学技术厅重点科技研发项目（20180201039NY）；吉林省畜禽良种工程项目（肉用草原红牛培育）；吉林省农业科学院国际合作项目（CXGC202108GH）；吉林省农业科技创新工程（高品质肉牛培育）

Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle

MA Yanru^1,2, YU Yongsheng², CAO Yang², QIN Lihong², ZHAO Yumin^2,3,4,5, WU Jian^2,3,4,5

1. College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
2. Jilin Academy of Agricultural Sciences, Gongzhuling 130033, China;
3. Key Laboratory of Beef Cattle Genetics and Breeding, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, China;
4. Northeast Cattle Performance Testing Center, Ministry of Agriculture and Rural Affairs, Gongzhuling 136100, China;
5. Jilin Beef Cattle Breeding and Breeding Technology Innovation Center, Gongzhuling 136100, China

Received:2021-10-31 Online:2022-06-05 Published:2022-05-27

摘要/Abstract

摘要： 【目的】研究中国草原红牛丙酮酸脱氢酶激酶4（pyruvate dehydrogenase kinase 4，PDK4）基因多态性与肉质性状的关系。【方法】挑选120头中国草原红牛为研究对象，采用Sanger测序法检测PDK4基因第1~11外显子的单核苷酸多态性（SNP）位点，分析SNP位点的基因型频率、基因频率及群体遗传参数等。利用SPSS 21.0软件对中国草原红牛PDK4基因SNP位点多态性与肉质性状（熟肉率、肉嫩度、失水率、pH、滴水损失、肌内脂肪含量、初水分含量、蛋白质含量）进行关联分析。【结果】在中国草原红牛PDK4基因外显子8和外显子11上共检测到3个SNPs；PDK4基因第8外显子57 bp处存在1个SNP位点（G57C），且引起编码氨基酸的改变，存在GG、GC和CC 3种基因型；PDK4基因第11外显子在330和389 bp处存在2个SNPs位点（G330T和C389T），在G330T位点上存在GG、GT和TT 3种基因型；在C389T位点上存在CC、CT和TT 3种基因型。卡方适合性检验结果显示，中国草原红牛第8外显子G57C位点偏离Hardy-Weinberg平衡状态（P<0.05），第11外显子的G330T和C398T符合Hardy-Weinberg平衡状态（P>0.05）；群体遗传参数分析发现，第8外显子G57C突变位点属于低度多态性位点（PIC<0.25），等位基因数为1.1429，表明其在中国草原红牛中的变异较小；第11外显子G330T和C398T属于中度多态性位点（0.25<PIC<0.5），等位基因数分别为1.6431和1.6447，说明该遗传标记能够提供遗传信息。关联分析结果表明，PDK4基因第8外显子中G57C位点GG和CC基因型个体肌内脂肪含量显著高于GC基因型（P<0.05）；第11外显子G330T位点GG基因型滴水损失和初水分量显著高于GT基因型（P<0.05）；GG基因型肉嫩度显著高于TT基因型（P<0.05）；GT基因型肌内脂肪含量显著高于TT基因型（P<0.05），C389T处CT基因型失水率显著低于TT基因型（P<0.05）。【结论】 PDK4基因多态性与中国草原红牛肉质性状相关，可作为肉质性状的候选基因。

关键词: PDK4基因; 中国草原红牛; 肉质性状; 多态性; 关联分析

Abstract: 【Objective】 The aim of the expriment was to study on the relationship between PDK4 gene polymorphism and meat quality traits in Chinese Steppe Red cattle.【Method】 120 Chinese Steppe Red cattle were selected as the research object.The single nucleotide polymorphism (SNP) of exons 1-11 of PDK4 gene was detected by Sanger sequencing, and the genotype frequency, gene frequency and population genetic parameters of SNP were analyzed.SPSS 21.0 software was used to analyze the association between SNP polymorphism of PDK4 gene and meat quality traits (cooked meat rate, meat tenderness, water loss rate, pH, drip loss, intramuscular fat content, initial water content and protein content) in Chinese Steppe Red cattle.【Result】 Three SNPs were detected in exons 8 and 11 of PDK4 gene in Chinese Steppe Red cattle.There was a SNP site at 57 bp (G57C) in exon 8 of PDK4 gene, which caused the change of coding amino acids.There were GG, GC and CC genotypes at G57C site.There were two SNPs at 330 bp(G330T) and 389 bp(C389T) in exon 11 of PDK4 gene.There were three genotypes of GG, GT and TT at G330T and three genotypes CC, CT and TT at C389T.Chi-square test results showed that the G57C site of exon 8 of Chinese Steppe Red cattle deviated from Hardy-Weinberg equilibrium (P<0.05), and the G330T and C398T of exon 11 conformed to Hardy-Weinberg equilibrium (P>0.05).The analysis of population genetic parameters showed that the G57C site in exon 8 belonged to low degree polymorphism (PIC<0.25), and the number of alleles was 1.1429, indicating that it had little variation in Chinese Steppe Red cattle.Exon 11 G330T and C398T belong to moderate polymorphism sites (0.25<PIC<0.5), and the number of alleles were 1.6431 and 1.6447 respectively, indicating that the genetic marker could provide genetic information.The association analysis results showed that the intramuscular fat content of GG and CC genotypes at G57C site in exon 8 of PDK4 gene was significantly higher than that of GC genotype (P<0.05).The drip loss and initial water component of GG genotype at G330T in exon 11 were significantly higher than those of GT genotype (P<0.05).The meat tenderness of GG genotype was significantly higher than that of TT genotype (P<0.05).The intramuscular fat content of GT genotype was significantly higher than that of TT genotype (P<0.05), and the dehydration rate of CT genotype at C389T was significantly lower than that of TT genotype (P<0.05).【Conclusion】 PDK4 gene polymorphism was associated with meat quality traits in Chinese Steppe Red cattle, and could be used as a candidate gene for meat quality traits.

Key words: PDK4 gene; Chinese Steppe Red cattle; meat quality traits; polymorphism; association analysis

中图分类号:

S813.3

马彦茹, 于永生, 曹阳, 秦立红, 赵玉民, 吴健. 中国草原红牛PDK4基因多态性及其与肉质性状的关联分析[J]. 中国畜牧兽医, 2022, 49(6): 2186-2194.

MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.

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中国草原红牛PDK4基因多态性及其与肉质性状的关联分析

Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle

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