鹅源副鸡禽杆菌的分离鉴定及致病性研究

doi:10.16431/j.cnki.1671-7236.2023.06.040

摘要/Abstract

摘要： 【目的】了解广东地区鹅源副鸡禽杆菌（Avibacterium paragallinarum，Apg）的生物学特性及其与国内外Apg菌株的差异。【方法】于2019年12月― 2022年1月从广东地区采集疑似鹅传染性鼻炎病例，通过特异性PCR扩增、细菌分离纯化及16S rRNA基因测序鉴定后进行比对并构建系统进化树，确定分离菌株种类并对其进行药敏试验和动物回归试验。【结果】本试验共分离鉴定获得3株鹅源Apg，分别命名为G-AP1、G-AP2、G-AP3，均属于NAD依赖型菌株。分离菌株发酵葡萄糖、蔗糖产酸不产气，氧化酶试验阳性。系统进化树结果显示，3株分离菌株血清型均为A型且亲缘关系较近，与国内大部分分离株亲缘关系较近，与国外分离株亲缘关系较远。分离菌株接种试验鹅，24 h后鹅出现面部肿胀与流鼻液，48 h后挤压肿胀部位可见乳白色絮状的黏稠鼻液流出，剖检病鹅可见肿胀部皮下和鼻窦、鼻甲黏膜水肿、出血、纤维性渗出，鼻窦腔蓄积干酪样渗出物。药敏试验结果表明，头孢噻呋、头孢噻诺、阿米卡星、喹诺酮类药物、氟苯尼考和克林霉素对3株分离菌株具有较强的体外生长抑制作用。【结论】本试验成功分离3株鹅源Apg菌株，可引起鹅群感染发病且具有传染性，研究结果为广东地区防治鹅传染性鼻炎提供了参考依据。

关键词: 鹅; 副鸡禽杆菌(Apg); 分离鉴定; 回归试验; 药敏试验

Abstract: 【Objective】 This study was amied to study biological characteristics of Avibacterium paragallinarum (Apg) in Guangdong province and its differences with Apg strains in China and abroad.【Method】 Suspected cases of infectious rhinitis of geese were collected from Guangdong from December 2019 to January 2022.After specific PCR amplification, bacterial isolation and purification, 16S rRNA gene sequencing and identification, sequence comparison was conducted and phylogenetic tree was constructed to determine the type of the isolated strain, and drug sensitivity test and animal regression test were conducted on the isolated strain.【Result】 A total of 3 strains from goose derived Apg were isolated and identified, named G-AP1, G-AP2 and G-AP3, respectively, which all belonged to NAD-dependent strains.The isolated strain fermented glucose and sucrose to produce acid but not gas, and the oxidase test was positive.The results of phylogenetic tree showed that the serotypes of the three isolates were all type A and were closely related to each other, which were close to most of the isolates in China and distant to the isolates abroad.Isolated strains infected geese, the geese showed facial swelling and runny nose fluid after 24 h and milky white and flocculent nasal fluid flowed out from the swollen part when squeezed after 48 h.The infected geese showed subcutaneous swelling, edema, bleeding, fibrous exudation and caseous exudate accumulated in the sinus cavity.The drug sensitivity test showed that ceftiofurme, ceftianol, amikacin, quinolones, flufenicol and clindamycin had strong growth inhibition on the three isolates in vitro.【Conclusion】 In this study, three strains of Apg from geese were successfully isolated, which could cause infection and infectivity of geese.The results provided a reference for the prevention and control of infectious rhinitis of geese in Guangdong.

Key words: goose; Avibacterium paragallinarum(Apg); isolation and identification; regression test; drug sensitivity test

中图分类号:

S852.61

卢玉葵, 刘佳琪, 钟嘉诚, 陈济铛, 朱婉君, 张溢珊, 张济培. 鹅源副鸡禽杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2023, 50(6): 2562-2572.

LU Yukui, LIU Jiaqi, ZHONG Jiacheng, CHEN Jidang, ZHU Wanjun, ZHANG Yishan, ZHANG Jipei. Isolation,Identification and Pathogenicity Analysis of Avibacterium paragallinarum from Geese[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2562-2572.

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