滇西树鼩源沙门氏菌的分离鉴定及生物学特性研究

doi:10.16431/j.cnki.1671-7236.2023.04.042

摘要/Abstract

摘要： 【目的】确定试验滇西树鼩患病致死的原因,分析分离菌株的致病性与耐药情况。【方法】本研究按常规平板划线法对发病死亡的滇西树鼩心脏血、肝脏、脾脏、肺脏和肾脏进行病原菌分离,进一步对分离菌进行形态学观察、生化试验及16S rRNA基因鉴定,并进行药敏试验和致病性试验。【结果】从发病死亡的滇西树鼩肝脏和肺脏组织分离得到1株细菌。分离菌在麦康凯培养基上呈浅橘黄色单菌落,镜检为革兰氏阴性短杆菌。生化试验结果显示,分离菌能分解葡萄糖,不发酵乳糖,产气,产硫化氢等,符合沙门氏菌生化特性。遗传进化分析显示,分离株与肉用仔鸡源沙门氏菌CP082916菌株亲缘关系最近,16S rRNA基因核苷酸相似性为99%。药敏试验结果显示,分离菌对氯霉素、庆大霉素、新霉素、诺氟沙星等药物敏感;对克林霉素、万古霉素不敏感。致病性试验结果显示,该菌对树鼩及成年小鼠具有较强致病性,对小鼠的半数致死量(LD₅₀)为1.6×10⁶ CFU。病理组织学观察显示,感染组树鼩和小鼠肺脏肺泡间隔增宽、间隔内可见炎性细胞浸润;肝脏细胞排列紊乱,见大量坏死细胞崩解,窦状隙变形或减小,中央静脉淤血,周围有炎性细胞浸润;脾脏部分细胞结构紊乱。【结论】沙门氏菌是引起这次试验滇西树鼩发病的致死原因,分离菌对氯霉素、庆大霉素、新霉素敏感,具有较强的致病性。

关键词: 滇西树鼩; 沙门氏菌; 分离鉴定; 16S rRNA; 耐药性

Abstract: 【Objective】 The purpose of this study was to determine the cause of death of the Tupaia belangeri chinensis,and to find out the pathogenicity and drug resistance of isolated strain.【Method】 In this study,the pathogenic bacteria were isolated from the heart blood,liver,spleen,lung and kidney of diseased and dying Tupaia belangeri chinensis by the conventional plate scribing method.The strain was identified by morphological observation,biochemical test and identification of 16S rRNA gene.And drug sensitivity test and pathogenicity test were carried out.【Result】 A suspicious bacterial strain was isolated from the liver and lung of Tupaia belangeri chinensis,which died of illness.The isolate showed a single colony of light orange on McConkey medium,which was detected as Gram-negative short bacillus by microscopy.The biochemical test results showed that the isolated bacteria could decompose glucose,did not ferment lactose,produce gas,produce hydrogen sulfide,etc.,which conformed to the biochemical characteristics of Salmonella.Genetic evolution analysis showed that the isolate was closely related to the Salmonella CP082916 strain from broiler chickens,and the nucleotide similarity was 99%.The results of drug sensitivity test showed that the isolate was sensitive to chloramphenicol,gentamicin,neomycin,norfloxacin and other drugs,not sensitive to clindamycin and vancomycin.The results of pathogenicity test showed that the isolate had strong pathogenicity to Tupaia belangeri chinensis and adult mice,and the median lethal dose (LD₅₀) to mice was 1.6×10⁶ CFU.Histopathological observation showed that the alveolar septum of the lung of Tupaia belangeri chinensis and mice widened and inflammatory cells infiltrated in the septum.The arrangement of liver cells was disordered,with a large number of necrotic cells disintegrating,sinus space being deformed or reduced,blood stasis in the central vein,and inflammatory cells infiltrating around,some spleen cells were disordered.【Conclusion】 Salmonella was the key cause of the disease of the laboratory Tupaia belangeri chinensis and the isolated was sensitive to chloramphenicol,gentamicin and neomycin and had strong pathogenicity.

Key words: Tupaia belangeri chinensis; Salmonella; isolation and identification; 16S rRNA; drug resistance

中图分类号:

S852.61

曹颖颖, 梁亮, 曾怡蓉, 钟清华, 袁小芳, 唐海波, 冷静. 滇西树鼩源沙门氏菌的分离鉴定及生物学特性研究[J]. 中国畜牧兽医, 2023, 50(4): 1718-1728.

CAO Yingying, LIANG Liang, ZENG Yirong, ZHONG Qinghua, YUAN Xiaofang, TANG Haibo, LENG Jing. Isolation,Identification and Biological Characteristics of Salmonella from Tupaia belangeri chinensis[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1718-1728.

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