河北地区动物源大肠杆菌多重耐药性与流行性分析

doi:10.16431/j.cnki.1671-7236.2023.03.023

摘要/Abstract

摘要： 【目的】了解河北地区动物源大肠杆菌分离菌株的耐药情况和各耐药菌株之间的亲缘关系,为有效防控大肠杆菌耐药性的产生提供科学依据。【方法】从河北省某养殖场采集健康动物的粪便样本297份(鸡源48份、猪源249份),通过细菌分离纯化和PCR扩增对分离菌株进行鉴定,采用药敏试验确定分离菌株的耐药表型,并对其进行耐药基因检测、血清型鉴定、系统发育群分析、质粒复制子分型和基因分型鉴定。【结果】分离菌株在麦康凯培养基上呈粉红色圆形菌落,经16S rDNA测序分析鉴定出129株大肠杆菌,其中92株大肠杆菌呈现多重耐药现象,主要以3~4耐为主,耐药基因以floR、tetA、qnrS和strB的检出率最高;多重耐药大肠杆菌以O132为优势血清型,系统进化群主要分布在A和B1群,主要流行质粒为IncFIB和IncN;经MLST分型获得27个ST型,主要为ST93型,其中12株序列型为未知型。【结论】河北地区健康动物肠道内大肠杆菌多重耐药现象较为严重,其血清型众多,分子特征呈现多样性,仍需加强对动物源大肠杆菌耐药性和分子特征的监测。

关键词: 大肠杆菌; 耐药性; 分子特征

Abstract: 【Objective】 This study was aimed to investigate the drug resistance of animal-derived Escherichia coli (E.coli) isolates in Hebei and the genetic relationship among the drug-resistant strains after the prohibition of resistance,so as to provide a scientific basis for the effective prevention and control of E.coli drug resistance.【Method】 297 fecal samples (48 from chickens and 249 from pigs) of healthy animals were collected from a farm in Hebei province.The isolates were identified by bacterial isolation and purification and PCR amplification,and the resistance of the isolates were determined by drug susceptibility test.Drug resistance gene detection,serotype identification,phylogenetic group analysis,plasmid replicon typing and genotyping were performed.【Result】 The isolates appeared as pink round colonies on MacConkey medium.A total of 129 strains of E.coli were isolated and identified by 16S rDNA sequencing analysis,of which 92 strains of E.coli showed multi-drug resistance,mainly 3-4 resistance,and the detection rate of the drug resistance genes were the highest with floR,tetA,qnrS and strB.The multidrug-resistant E.coli was dominated by O132 serotype,and the phylogenetic groups were mainly distributed in groups A and B1,and the main prevalent plasmids were IncFIB and IncN,27 ST types were obtained by MLST typing,mainly ST93 type,of which 12 sequence types were unknown type.【Conclusion】 The phenomenon of multi-drug resistance of E.coli in the intestine of healthy animals in Hebei was relatively serious,with numerous serotypes and diverse molecular characteristics,and it was still necessary to strengthen the monitoring of drug resistance and molecular characteristics of E.coli of animal origin.

Key words: Escherichia coli; drug resistance; molecular characteristics

中图分类号:

S852.61⁺2

朱琪琪, 张继瑜, 王承业, 吴灵玉, 王玮玮, 朱阵. 河北地区动物源大肠杆菌多重耐药性与流行性分析[J]. 中国畜牧兽医, 2023, 50(3): 1081-1092.

ZHU Qiqi, ZHANG Jiyu, WANG Chengye, WU Lingyu, WANG Weiwei, ZHU Zhen. Analysis of Multidrug Resistance and Prevalence of Animal-derived Escherichia coli in Hebei[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(3): 1081-1092.

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