›› 2016, Vol. 43 ›› Issue (4): 1045-1049.doi: 10.16431/j.cnki.1671-7236.2016.04.029

• 遗传繁育 • 上一篇    下一篇

PRKAG2基因多态性及其与北京鸭经济性状的关联分析

张春雷1,2,3, 周嫄2, 房兴堂2, 侯水生4, 张桂英3, 陈宏2, 陈国宏1   

  1. 1. 扬州大学动物科技学院, 扬州 225009;
    2. 江苏师范大学生命科学学院, 徐州 221116;
    3. 徐州万佳牧业科技发展有限公司, 徐州 221116;
    4. 中国农业科学院北京畜牧兽医研究所, 北京 100193
  • 收稿日期:2015-09-22 出版日期:2016-04-20 发布日期:2016-04-27
  • 通讯作者: 陈国宏 E-mail:ghchen@yzu.edu.cn
  • 作者简介:张春雷(1980-),男,江苏邳州人,博士,副教授,研究方向:动物遗传育种与繁殖,E-mail:clzhang@jsnu.edu.cn
  • 基金资助:
    中国博士后基金(2013M541739);国家水禽产业体系项目(CARS-43)

Polymorphism of PRKAG2 Gene and its Association Analysis with Economic Traits in Peking Duck

ZHANG Chun-lei1,2,3, ZHOU Yuan2, FANG Xing-tang2, HOU Shui-sheng4, ZHANG Gui-ying3, CHEN Hong2, CHEN Guo-hong1   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. School of Life Sciences, Jiangsu Normal University, Xuzhou 221116, China;
    3. Xuzhou Wanjia Livestock Science and Technology Development Co., Ltd., Xuzhou 221116, China;
    4. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
  • Received:2015-09-22 Online:2016-04-20 Published:2016-04-27

摘要: 本研究利用PCR-SSCP及DNA测序技术对334个北京鸭个体已报道的重要能量代谢调控基因——PRKAG2基因进行遗传变异的检测,分析其多态性,并使用SPSS 19.0将北京鸭的经济性状与PRKAG2多态性进行了关联分析。结果表明,在北京鸭PRKAG2基因位点发现1个SNP (A>G),该位点位于PRKAG2基因3'-非翻译区,有3种基因型:AA、AB、BB,其中AA基因型为优势基因型。与经济性状的相关性分析发现,PRKAG2基因的遗传多态性对北京鸭的18日龄重、胸宽有极显著影响(P<0.01),对龙骨长有显著影响(P<0.05),可作为北京鸭分子育种的辅助标记,也可为改善鸭性能提供参考。

关键词: 北京鸭; PRKAG2基因; 经济性状; PCR-SSCP; 关联分析

Abstract: PCR-SSCP and DNA sequencing technology were used to investigate the polymorphism of PRKAG2 gene in the Peking duck population with 334 birds.One SNP (A>G) of PRKAG2 gene was detected, which located at the 3'-untranslating region of PRKAG2 gene, there were three genotypes:AA, AB and BB, and AA genotype was the dominant genotype.It had extremely significant association with weight of 18 days old and chest width (P<0.01), and had significant association with keel length (P<0.05).So this polymorphic locus might serve as a molecular marker for Peking duck selection, and could provide references for improvement of duck performance.

Key words: Peking duck; PRKAG2 gene; economic traits; PCR-SSCP; association analysis

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