GnIH与INH表位多肽疫苗主动免疫对甘肃高山细毛羊生殖激素的影响

doi:10.16431/j.cnki.1671-7236.2016.04.028

摘要/Abstract

摘要： 本试验通过合成促性腺激素类的抑制激素——抑制素(inhibit hormone,INH)与促性腺激素抑制激素(gonadotropin inhibiting hormone,GnIH)的INH表位多肽疫苗和GnIH表位多肽疫苗,主动免疫甘肃高山细毛羊并对促卵泡素(follicle stimulating hormone,FSH)和促黄体素(luteinizing hormone,LH)激素水平进行比较分析。试验选用15只处在非发情季节的3.5岁的甘肃高山细毛羊,注射INH表位多肽疫苗与GnIH表位多肽疫苗分别为表位多肽疫苗A组和表位多肽疫苗B组,对照组注射等量生理盐水。采血后进行免疫,每隔7 d免疫和采血,同时在发情后每隔15 min采集一次血液,分离血清,用ELISA试剂盒检测抗体水平及FSH和LH激素变化。结果表明,表位多肽疫苗A组和表位多肽疫苗B组在初次免疫后都产生了相应的抗体,且表位多肽疫苗B组较表位多肽疫苗A组产生的抗体浓度高,75 、90、105 min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了FSH的分泌水平(P<0.05)。在45~105 min时,表位多肽疫苗B组相对表位多肽疫苗A组显著促进了LH的分泌水平(P<0.05),且在90到105 min时表位多肽疫苗A组和表位多肽疫苗B组血清中FSH和LH的水平均显著高于对照组(P<0.05)。本试验结果表明,INH表位多肽疫苗和GnIH表位多肽疫苗主动免疫促进了甘肃高山细毛羊的FSH、LH的分泌,GnIH表位多肽疫苗在甘肃高山细毛羊上具有更好的免疫作用,本研究为该表位多肽疫苗在绵羊上的运用奠定了基础。

关键词: 促性腺激素抑制激素; 抑制素; 主动免疫; 甘肃高山细毛羊; 生殖激素

Abstract: This study aimed to detect the reproductive hormones levels of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) in Gansu Alpine Fine-wool sheep by active immunization of two synthesized epitope peptide vaccines A and B which were combined inhibit hormone (INH) and gonadotropin inhibit hormone (GnIH).15 Gansu Alpine Fine-wool sheep were selected which were 3.5 year old and lived in anoestrus season, 3 groups each had 5 sheep, GnIH epitope peptide vaccine and INH epitope peptide vaccine were named epitope peptide vaccine A and B groups, while blank group was injected with saline.The blood was collected before immunization and immuned, and bloods were collected once every 7 days, meanwhile collected blood every 15 min after estrus.The serum was used to detect antibody, FSH and LH levels by ELISA Kits.The results showed that the epitope peptide vaccine A and B groups produced the corresponding antibodies after the initial immunization, and epitope peptide vaccine B group produced more antibodies than epitope peptide vaccine A group.Epitope peptide vaccine B group promoted the FSH secretion levels was significantly higher than epitope peptide vaccine A group in 75, 90 and 105 min (P<0.05);In 45 to 105 minutes, the LH secretion levels of epitope peptide vaccine B group was significantly higher than epitope peptide vaccine A group (P<0.05), while the LH and FSH levels in epitope peptide vaccine A and B groups were significantly higher than control group (P<0.05).This study indicated that the epitope peptide vaccine A and B groups could promote the FSH and LH secretion levels through active immunity in Gansu Alpine Fine-wool sheep and had a good immune function.This study laid a foundation for the application of epitope peptide vaccine on sheep.

Key words: gonadotropin inhibit hormone (GnIH); inhibit hormone (INH); active immune; Gansu Alpine Fine-wool sheep; reproductive hormones

中图分类号:

Q575⁺.12

张玲玲, 杨博辉, 岳耀敬, 郭婷婷, 刘建斌, 袁超, 牛春娥, 冯瑞林, 郭健, 孙晓萍, 刘善博. GnIH与INH表位多肽疫苗主动免疫对甘肃高山细毛羊生殖激素的影响[J]. , 2016, 43(4): 1039-1044.

ZHANG Ling-ling, YANG Bo-hui, YUE Yao-jing, GUO Ting-ting, LIU Jian-bin, YUAN Chao, NIU Chun-e, FENG Rui-lin, GUO Jian, SUN Xiao-ping, LIU Shan-bo. Effects of Active Immunization with GnIH and INH Epitope Peptide Vaccine on Reproductive Hormones of Gansu Alpine Fine-wool Sheep[J]. , 2016, 43(4): 1039-1044.

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