THBS3基因在长白猪和通城猪不同组织和不同时期骨骼肌中的表达分析

doi:10.16431/j.cnki.1671-7236.2016.04.027

›› 2016, Vol. 43 ›› Issue (4): 1032-1038.doi: 10.16431/j.cnki.1671-7236.2016.04.027

THBS3基因在长白猪和通城猪不同组织和不同时期骨骼肌中的表达分析

马喜山¹, 唐中林², 肖冲¹, 杨博超¹, 秦川¹

1. 中国医学科学院医学实验动物研究所, 北京 100021;
2. 中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2015-10-26 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 秦川 E-mail:chuanqin@vip.sina.com
作者简介:马喜山(1982-),男,山东青岛人,博士,助理研究员,研究方向:动物分子遗传学,E-mail:xishanm@163.com
基金资助:
国家自然科学基金(31201787)

Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs

MA Xi-shan¹, TANG Zhong-lin², XIAO Chong¹, YANG Bo-chao¹, QIN Chuan¹

1. Institute of Laboratory Animal Sciences, China Academy of Medical Sciences, Beijing 100021, China;
2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China

Received:2015-10-26 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 本研究主要探究血小板反应蛋白3 (thrombospondin-3,THBS3)基因在不同组织及不同时期骨骼肌生长发育过程中的表达规律。利用实时荧光定量PCR方法对THBS3基因在长白猪和通城猪中的组织表达谱,以及在胚胎期(33、45、65、70和90 d)和出生后阶段(0、9、30、60、120和160 d)骨骼肌中的差异表达进行了比较分析。结果表明,THBS3基因广泛表达于长白猪和通城猪各个组织器官,除胃和肠中的表达存在差异外,其在两个猪种中的组织表达谱基本一致,在肺脏中表达量最高。THBS3基因在长白猪和通城猪胚胎期骨骼肌中的表达水平均显著高于出生后阶段(P<0.05),但胚胎期骨骼肌中的表达模式在两个猪种间存在一定差异,THBS3基因表达峰值出现在长白猪胚胎期45 d,而其在通城猪中表达峰值出现在胚胎期65 d。结果提示,THBS3基因参与了猪骨骼肌生长发育过程及不同类型猪种骨骼肌生长发育异步性的调控。

关键词: 猪; THBS3基因; 骨骼肌; 实时荧光定量PCR; 表达

Abstract: To study the expression pattern of THBS3 gene in different tissues and during skeletal muscle development, the THBS3 gene expression in different tissues and skeletal muscles during prenatal periods (33, 45, 65, 70 and 90 d) and postnatal periods (0, 9, 30, 60, 120 and 160 d) from Landrace and Tongcheng pigs were detected by Real-time quantification PCR.The results showed that THBS3 gene widely expressed in all tissues examined, exhibiting similar spatial expression patterns with expression peaks in lung in both pig breeds except in stomach and intestine.Moreover, although THBS3 gene showed a significant higher expression level in gestation than after birth in Landrace and Tongcheng pigs (P<0.05), it exhibited different expression patterns between Landrace and Tongcheng pigs, the expression peak was detected at gestation day 45 in Landrace pig, while was detected at gestation day 65 in Tongcheng pig.The results suggested that THBS3 gene involved in skeletal muscle growth and development in pigs, as well as the regulation of asynchronization of skeletal muscle development in different pig breeds.

Key words: pig; THBS3 gene; skeletal muscle; Real-time quantification PCR; expression

中图分类号:

Q786

马喜山, 唐中林, 肖冲, 杨博超, 秦川. THBS3基因在长白猪和通城猪不同组织和不同时期骨骼肌中的表达分析[J]. , 2016, 43(4): 1032-1038.

MA Xi-shan, TANG Zhong-lin, XIAO Chong, YANG Bo-chao, QIN Chuan. Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs[J]. , 2016, 43(4): 1032-1038.

参考文献

[1] Wigmore P M,Stickland N C.Muscle development in large and small pig fetuses[J].J Anat, 1983,137(2):235-245.
[2] Te Pas M F,De Wit A A,Priem J,et al.Transcriptome expression profiles in prenatal pigs in relation to myogenesis[J].J Muscle Res Cell Motil,2005,26(2-3):157-165.
[3] Stickland N C,Widdowson E M,Goldspink G.Effects of severe energy and protein deficiencies on the fibres and nuclei in skeletal muscle of pigs[J]. Br J Nutr,1975,34(3):421-428.
[4] Denise C H,Patricia A T,Ronen S,et al.Extracellular matrix fibronectin mechanically couples skeletal muscle contraction with local vasodilation[J].Circ Res,2008,102(3):372-379.
[5] Joshi S K,Lee L,Lovett D H,et al.Novel intracellular N-terminal truncated matrix metalloproteinase-2 isoform in skeletal muscle ischemia-reperfusion injury[J].J Orthop Res, 2015,7(24):22992.
[6] Nishimura T.Role of extracellular matrix in development of skeletal muscle and postmortem aging of meat[J].Meat Sci,2015,12(109):48-55.
[7] Tang Z,Li Y,Wan P,et al.Long SAGE analysis of skeletal muscle at three prenatal stages in Tongcheng and Landrace pigs[J].Genome Biol,2007,8(6):R115.
[8] Ma X,Tang Z,Wang N,et al.Identification of extracellular matrix and cell adhesion molecule genes associated with muscle development in pigs[J].DNA and Cell Biol,2011,30(7):469-479.
[9] Patruno M,Caliaro F,Maccatrozzo L,et al.Myostatin shows a specific expression pattern in pig skeletal and extraocular muscles during pre-and post-natal growth[J].Differentiation,2008,76(2):168-181.
[10] Costa A,Afonso J,Osório C,et al.miR-363-5p regulates endothelial cell properties and their communication with hematopoietic precursor cells[J].J Hematol Oncol,2013,6(1):87-98.
[11] Gao B,Sun W,Wang X,et al.Whole genome expression profiling and screening for differentially expressed cytokine genes in human bone marrow endothelial cells treated with humoral inhibitors in liver cirrhosis[J].Int J Mol Med,2013,32(5):1204-1214.
[12] Simanovsky M,Berlinsky S,Pirchia S,et al.Phenotypic and gene expression diversity of malignant cells in human blast crisis chronic myeloid leukemia[J]. Differentiation,2008,76(8):908-922.
[13] Ching S,Zhang H,Chen Q,et al.Differential expression of extracellular matrix and adhesion molecule genes in the brain of juvenile versus adult mice in responses to intracerebroventricular administration of IL-1[J].Neuroimmunomodulation,2007,14(1):46-56.
[14] Hankenson K D,Hormuzdi S G,Meganck J A,et al.Mice with a disruption of the thrombospondin 3 gene differ in geometric and biomechanical properties of bone and have accelerated development of the femoral head[J].Mol Cell Biol,2005,25(13):5599-5606.
[15] Hankenson K D,Sweetwyne M T,Shitaye H,et al.Thrombospondins and novel TSR-containing proteins,R-spondins,regulate bone formation and remodeling[J].Curr Osteoporos Rep,2010,8(2):68-76.
[16] Posey K L,Hankenson K,Veerisetty A C,et al.Skeletal abnormalities in mice lacking extracellular matrix proteins,thrombospondin-1,thrombospondin-3,thrombospondin-5,and type IX collagen[J].Am J Pathol,2008,172(6):1664-1674.
[17] Adolph K W.Relative abundance of thrombospondin 2 and thrombospondin 3 mRNAs in human tissues[J].Biochem Biophys Res Commun,1999,258(3):792-806.
[18] Vos H L,Devarayalu S,de Vries Y,et al.Thrombospondin 3(THBS3),a new member of the thrombospondin gene family[J].J Biol Chem,1992,267(17):12192-12196.
[19] 马喜山,谭林,唐中林,等. TIMP3基因在长白猪和通城猪不同组织及不同时期骨骼肌中的表达分析[J].中国畜牧兽医,2011,38(12):149-154.
[20] Rehfeldt C,Fiedler I,Dietl G,et al.Myogenesis and postnatal skeletal muscle cell growth as influenced by selection[J].Livestock Production Science,2000,66(2):177-188.
[21] Adams J C,Watt F M,Watt F M.Regulation of development and differentiation by the extracellular matrix[J].Development,1993,117(4):1183-1198.
[22] Damsky C H,Moursi A,Zhou Y,et al.The solid state environment orchestrates embryonic development and tissue remodeling[J].Kidney Int,1997,51(5):1427-1433.

THBS3基因在长白猪和通城猪不同组织和不同时期骨骼肌中的表达分析

Expression Analysis of THBS3 Gene in Different Tissues and Skeletal Muscles at Different Periods from Landrace and Tongcheng Pigs

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