›› 2016, Vol. 43 ›› Issue (4): 1024-1031.doi: 10.16431/j.cnki.1671-7236.2016.04.026

• 遗传繁育 • 上一篇    下一篇

中国6个地方绵羊品种的微卫星分析

邱小宇, 黄勇富, 赵永聚, 娜日苏   

  1. 西南大学动物科技学院, 重庆 400716
  • 收稿日期:2015-10-13 出版日期:2016-04-20 发布日期:2016-04-27
  • 通讯作者: 黄勇富 E-mail:Hyf65@163.com
  • 作者简介:邱小宇(1992-),男,四川乐山人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:qiuxiaoyu4044@sina.com
  • 基金资助:
    中央高校基本科研业务费专项资金(SWU114023)

Microsatellite Analysis of Six Chinese Indigenous Sheep Breeds

QIU Xiao-yu, HUANG Yong-fu, ZHAO Yong-ju, NA Ri-su   

  1. College of Animal Science and Technology, Southwest University, Chongqing 400716, China
  • Received:2015-10-13 Online:2016-04-20 Published:2016-04-27

摘要: 为研究中国地方绵羊品种之间的遗传多样性,利用6个微卫星位点,对中国6个绵羊品种群体进行遗传检测,计算了各品种群体的平均杂合度(He)和平均多态信息含量(PIC),同时分析了各品种群体内的遗传变异,进行了UPGMA聚类。结果表明,6个绵羊品种Ho为0.5446~0.7140,PIC为0.5211~0.7732;6个绵羊品种都具有较丰富的遗传多样性,其中岷县黑裘皮羊的遗传变异最高,小尾寒羊的遗传变异最低;小尾寒羊的近交系数最高,揭示该群体内近交程度可能较高。利用UPGMA方法构建了系统发生树,兰州大尾寒羊与岷县黑裘皮羊为一类,哈萨克羊与贵德黑裘皮羊为一类,泗水绵羊与小尾寒羊为一类,表明群体遗传结构与地理分布及其驯化历史相关。

关键词: 微卫星; 遗传多样性; 绵羊

Abstract: This article described the relationship among six Chinese indigenous sheep breeds.There were six microsatellite loci which used to analyze the genetic diversity.Based on the allele frequency of six sheep variations, we calculated the mean heterozygosity (Ho), which were from 0.5446 to 0.7140;The polymorphism information content (PIC) in six sheep breeds were from 0.5211 to 0.7732.Genetic variations within breed were analyzed in the study, and we clustered the UPGMA tree according to the DA genetic distance.The results showed that six breeds had relatively abundant genetic diversity, and MXB had the highest genetic variation, while SW had the lowest genetic variation.The inbreeding coefficient of SW was higher than other breeds, which revealed a higher inbreeding degree in this breed.Phylogenetic tree was constructed using UPGMA, which showed that LZD and MXB were classified as a class, HZK and GF were classified as a class, SIH and SW were classified as a class.The results revealed the genetic structure of breeds correlated with geographical distribution and history of domestication.

Key words: microsatellite; genetic diversity; sheep

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