不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析

doi:10.16431/j.cnki.1671-7236.2016.04.025

›› 2016, Vol. 43 ›› Issue (4): 1017-1023.doi: 10.16431/j.cnki.1671-7236.2016.04.025

不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析

夏日炜¹, 甘丽娜¹, 钦伟云¹, 孙寿永¹, 包文斌^1,2, 吴圣龙^1,2

1. 扬州大学动物科学与技术学院, 江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009;
2. 江苏省种猪繁育和健康养殖工程技术研究中心, 扬州 225009

收稿日期:2015-10-27 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 吴圣龙 E-mail:slwu@yzu.edu.cn
作者简介:夏日炜(1992-),男,江苏兴化人,硕士生,研究方向:猪抗病育种,E-mail:xrw19920814@163.com
基金资助:
国家自然科学基金(31572360、31472066);江苏省科技支撑计划(BE2015329、BE2014357);江苏省农业三项工程(SXGC[2015]326)

Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages

XIA Ri-wei¹, GAN Li-na¹, QIN Wei-yun¹, SUN Shou-yong¹, BAO Wen-bin^1,2, WU Sheng-long^1,2

1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
2. Jiangsu Engineering Research Center for the Reproduction and Healthy Breeding of Boar, Yangzhou 225009, China

Received:2015-10-27 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 试验旨在探讨LTβR基因在仔猪出生至断奶期间的mRNA表达变化,为进一步研究该基因与F18大肠杆菌致病的相关性提供理论依据。本试验选取从初生到断奶的4个日龄(8、18、30和35日龄)苏太仔猪各4头,利用实时荧光定量PCR方法分别比较分析了LTβR基因在各个体11个组织(心脏、肝脏、脾脏、肺脏、肾脏、胃、肌肉、胸腺、淋巴结、十二指肠及空肠)间的表达规律。结果表明,LTβR基因在肝脏、脾脏、肺脏、肾脏、胃、淋巴结、十二指肠及空肠组织中呈现较高水平的表达,并且表现出明显的发育性表达差异。LTβR基因在8日龄仔猪淋巴、十二指肠和空肠组织中的表达极显著高于其他日龄(P<0.01);在8日龄仔猪肺脏组织中的表达极显著高于35日龄(P<0.01),且显著高于30日龄(P<0.05);在35日龄仔猪肝脏组织中的表达极显著高于其他日龄(P<0.01);在35日龄仔猪胃组织中的表达显著高于18日龄(P<0.05)。由此推测,8日龄左右为仔猪肠道免疫屏障快速形成期,LTβR基因的较高表达可能有利于仔猪对F18大肠杆菌抗性。

关键词: 猪; LTβR基因; F18大肠杆菌; 差异表达

Abstract: Dynamic changes of LTβR expression levels in 11 tissues (heart, liver, spleen, lung, kidney, stomach, muscle, thymus, lymph node, duodenum and jejunum) of Sutai piglets ranging from newborn to post-weaning days 8, 18, 30, and 35 were compared and analyzed by the Real-time PCR method, which aimed to provide theoretical basis for further investigate the relationship between LTβR gene and pathogenicity of E.coli F18.The results revealed that the LTβR expression levels were higher in the liver, spleen, lung, kidney, stomach, lymph node, duodenum and jejunum, and showed obvious age-dependent expression differentiation.The LTβR expression levels in the lymph node, duodenum, and jejunum were extremely significant higher in 8 days old piglets than in the other age stages (P<0.01), and the expression levels were extremely significantly higher in the lungs of 8 days old piglets than in 35 days old piglets (P<0.01) and significantly higher than 30 days old piglets (P<0.05).In the liver tissue, the expression level was extremely significant higher in 35 days old piglets than in other age stages (P<0.01).In the stomach tissue, the expression level was significantly higher in 35 days old piglets than in 18 days old piglets (P<0.05).The results speculated that intestinal immune barrier of piglets formed rapidly around 8 days old and the higher LTβR expression could contribute to the resistance to E.coli F18.

Key words: pigs; LTβR gene; E.coli F18; differential expression

中图分类号:

Q786

夏日炜, 甘丽娜, 钦伟云, 孙寿永, 包文斌, 吴圣龙. 不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析[J]. , 2016, 43(4): 1017-1023.

XIA Ri-wei, GAN Li-na, QIN Wei-yun, SUN Shou-yong, BAO Wen-bin, WU Sheng-long. Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages[J]. , 2016, 43(4): 1017-1023.

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不同日龄苏太仔猪LTβR基因组织表达谱及发育性表达规律分析

Tissue Expression Profile and Differential Expression of LTβR Gene in Sutai Piglets of Different Ages

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