猪免疫口蹄疫灭活疫苗后外周血细胞亚群变化的研究

doi:10.16431/j.cnki.1671-7236.2016.04.024

›› 2016, Vol. 43 ›› Issue (4): 1012-1016.doi: 10.16431/j.cnki.1671-7236.2016.04.024

猪免疫口蹄疫灭活疫苗后外周血细胞亚群变化的研究

张柳, 冯霞, 靳野, 祁淑芸, 张晓霞, 马军武

中国农业科学院兰州兽医研究所, 家畜疫病病原生物学国家重点实验室, 国家口蹄疫参考实验室, 兰州 730046

收稿日期:2015-12-11 出版日期:2016-04-20 发布日期:2016-04-27
通讯作者: 马军武 E-mail:majunwu@caas.cn
作者简介:张柳(1991-),女,河北邯郸人,硕士,研究方向:兽医病毒病诊断技术,E-mail:chenxiyixi@126.com
基金资助:
甘肃省科技支撑计划(1304NKCA150)

Study on Change of Lymphocyte Subpopulations in Swine after Immunization with Inactivated Vaccine

ZHANG Liu, FENG Xia, JIN Ye, QI Shu-yun, ZHANG Xiao-xia, MA Jun-wu

State Key Laboratory of Veterinary Etiological Biology, National Foot and Mouth Disease Reference Laboratory, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China

Received:2015-12-11 Online:2016-04-20 Published:2016-04-27

摘要/Abstract

摘要： 为了解动物在注射口蹄疫灭活疫苗后机体免疫状态的变化情况,本试验选取口蹄疫阴性猪进行疫苗免疫,利用流式细胞术检测方法来测定外周血中免疫细胞亚群的变化。结果显示,树突状细胞在免疫后能够快速产生应答,在24 h内其含量出现明显升高;B细胞和Th细胞含量在免疫后第10天都出现了明显升高,且两者之间的变化存在着高度相关性,而抗体水平的变化情况与这两种细胞含量的变化也高度相关;细胞毒性T细胞含量在免疫后也出现升高。这说明灭活疫苗免疫动物后不仅可以激活机体的体液免疫应答反应,产生高水平抗体,发挥免疫保护作用,更重要的是能够在短时间内激发机体的固有免疫应答,同时能够激活细胞免疫应答,全方位发挥免疫保护作用。

关键词: 口蹄疫病毒; Th细胞; CTL细胞; B细胞; 树突状细胞

Abstract: In order to investigate the change of lymphocyte subpopulations in the peripheral blood after injection of foot-and-mouth disease inactivated vaccine, blood samples were collected after vaccination and assayed with flow cytometry.The results showed that the proportion of dendritic cells (DCs) increased significantly in a short time after vaccination, after which the number of B cells, helper T (Th) cells and cytotoxic lymphocytes (CTLs) also raised with significance.The changing trend of B cells proportion was highly relevant with Th cells and a same correlation also appeared in the antibody titers.This indicated that the innate immune system could be activated rapidly after vaccination with the inactivated vaccine, and the adaptive immune response including cellular immune response and humoral immune response could be invoked subsequently.The high level of neutralizing antibodies was of great importance in evading FMDV and the cellular immune response also exerted a crucial role.

Key words: foot-and-mouth disease virus; Th cell; CTLs; B cell; DCs

中图分类号:

S852.65⁺9.6

张柳, 冯霞, 靳野, 祁淑芸, 张晓霞, 马军武. 猪免疫口蹄疫灭活疫苗后外周血细胞亚群变化的研究[J]. , 2016, 43(4): 1012-1016.

ZHANG Liu, FENG Xia, JIN Ye, QI Shu-yun, ZHANG Xiao-xia, MA Jun-wu. Study on Change of Lymphocyte Subpopulations in Swine after Immunization with Inactivated Vaccine[J]. , 2016, 43(4): 1012-1016.

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猪免疫口蹄疫灭活疫苗后外周血细胞亚群变化的研究

Study on Change of Lymphocyte Subpopulations in Swine after Immunization with Inactivated Vaccine

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