12C6+离子束辐照对茶树精油质量及其杀灭口蹄疫病毒效果的影响

doi:10.16431/j.cnki.1671-7236.2021.02.038

中国畜牧兽医 ›› 2021, Vol. 48 ›› Issue (2): 755-762.doi: 10.16431/j.cnki.1671-7236.2021.02.038

¹²C⁶⁺离子束辐照对茶树精油质量及其杀灭口蹄疫病毒效果的影响

梁妍^1,2, 陶蕾⁴, 宋向东³, 刘宇³, 赵凤舞⁴, 李雪虎², 辛志君², 陆锡宏², 梁剑平^2,3, 冯建强³, 周翔², 王震¹

1. 兰州大学药学院, 兰州 730000;
2. 中国科学院近代物理研究所, 兰州 730000;
3. 中国农业科学院兰州畜牧与兽药研究所, 兰州 730050;
4. 兰州职业技术学院, 兰州 730070

收稿日期:2020-05-07 出版日期:2021-02-20 发布日期:2021-02-23
通讯作者: 周翔, 王震 E-mail:syannovich@gmail.com;zhenw@lzu.edu.cn
作者简介:梁妍(1991-),女,山西平遥人,博士生,研究方向:药物化学,E-mail:liangy18@lzu.edu.cn;陶蕾(1974-),女,甘肃兰州人,博士,研究方向:中兽药开发,E-mail:1043989953@qq.com
基金资助:
兰州市科技计划项目（2016-3-101）；甘肃省自然科学基金项目（1506RJZA293）；中国科学院西部之光人才培养引进计划-"西部青年学者"（Ke-Fa-Ren-Zi[2015]No.77）；中国农业科学院2018年重大产出科研选题项目（CAAS-ZDXT2018008）；甘肃省中药材产业体系项目

Effects of ¹²C⁶⁺ Ion Beam Irradiation on the Quality of Tea Tree Essential Oil and Its Efficacy Against Foot-and-mouth Disease Virus

LIANG Yan^1,2, TAO Lei⁴, SONG Xiangdong³, LIU Yu³, ZHANG Fengwu⁴, LI Xuehu², XING Zhijun², LU Xihong², LIANG Jianping^2,3, FENG Jianqiang³, ZHOU Xiang², WANG Zhen¹

1. School of Pharmacy, Lanzhou University, Lanzhou 730000, China;
2. Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou 730000, China;
3. Lanzhou Institute of Husbandry and Pharmaceutical Sciences of CAAS, Lanzhou 730050, China;
4. Lanzhou Vocational Technical College, Lanzhou 730070, China

Received:2020-05-07 Online:2021-02-20 Published:2021-02-23

摘要/Abstract

摘要： 本研究旨在探索¹²C⁶⁺离子辐照茶树精油后，茶树精油活性成份的变化及其对口蹄疫病毒（foot-and-mouth disease virus，FMDV）的杀灭效果。采用50、100、150、200 Gy的¹²C⁶⁺离子束辐照茶树精油后，利用气相色谱-质谱联用仪（gas chromatography-mass spectrometer，GC-MS）法和红外色谱法分析辐照前后茶树精油主要成分变化及其质量沉积效应；检测茶树精油中松油烯-4-醇、1，8-桉叶素含量变化；将辐照前后茶树精油原液以及1：2、1：4辐照后稀释的精油注射50日龄成年鼠检测其临床用药的安全性；将稀释成1：2、1：4、1：6、1：8的辐照后茶树精油分别与等量的1：500的O-FMDV强毒充分混匀，皮下注射4~5日龄乳鼠，连续观察7 d，观察小鼠有无FMDV感染症状。试验结果表明，以100 Gy的¹²C⁶⁺离子束辐照茶树精油后，松油烯-4-醇含量由37.90%升高到39.03%，1，8-桉叶素含量无明显变化，羟基化合物含量明显增高；辐照前后茶树精油原液以及稀释后精油注射未见不良反应，辐照后茶树精油临床用药安全；乳鼠感染混有不同浓度的O-FMDV后，1：2、1：4稀释倍数的茶树精油组感染O-FMDV的乳鼠未出现死亡，注射部位未见炎症、坏死等，阳性对照组全部死亡。结果表明，¹²C⁶⁺离子束辐照茶树精油最佳剂量为100 Gy，在乳鼠体内抗O-FMDV效果的最大稀释度为1：4，¹²C⁶⁺离子束辐照可以提高茶树精油对FMDV的杀灭效果。

关键词: ¹²C⁶⁺离子束; 茶树精油; 质量沉积效应; 口蹄疫病毒(FMDV)

Abstract: The purpose of this study was to explore the changes of the active components of tea tree essential oil after ¹²C⁶⁺ ion injection and evaluate the killing effect of Foot-and-mouth disease virus (FMDV).Tea tree essential oil was irradiated by ¹²C⁶⁺ ion beam of 50,100,150 and 200 Gy,and the changes of main components and mass deposition effects of tea tree essential oil before and after irradiation were analyzed by gas chromatography-mass spectrometer (GC-MS) and infrared chromatography.The contents of terpinene-4-ol and 1,8-eucalyptin in tea tree essential oil were detected.The safety of tea tree essential oil before and after irradiation and the essential oil diluted by 1:2 and 1:4 irradiation were injected into suchling mice to detect the clinical use of tea tree essential oil. The irradiated tea tree essential oil diluted into 1:2, 1:4, 1:6 and 1:8 was thoroughly mixed with the same amount of O-FMDV at 1:500, respectively. The mice aged 4-5 d were subcutaneously injected and observed continuously for 7 d to observe whether the mice were infected with O-FMDV.The results showed that after irradiated tea tree essential oil with 100 Gy ¹²C⁶⁺ ion beam,the content of pinole-4 alcohol increased from 37.90% to 39.03%,the content of 1,8-eucalyptus had no significant change,and the content of hydroxyl compounds significantly increased after irradiated tea tree essential oil.The optimal irradiation dose was 100 Gy,and the tea tree essential oil with radiation dose of 100 Gy (the maximum dilution was 1:4) had the best anti-FMDV effect in suckling mouse.Irradiation of tea tree essential oil with a dose of 100 Gy could improve the killing effect of FMDV.

Key words: ¹²C⁶⁺ ion beam; tea tree essential oil; mass deposition effect; Foot-and-mouth disease virus (FMDV)

中图分类号:

R780.1

梁妍, 陶蕾, 宋向东, 刘宇, 赵凤舞, 李雪虎, 辛志君, 陆锡宏, 梁剑平, 冯建强, 周翔, 王震. ¹²C⁶⁺离子束辐照对茶树精油质量及其杀灭口蹄疫病毒效果的影响[J]. 中国畜牧兽医, 2021, 48(2): 755-762.

LIANG Yan, TAO Lei, SONG Xiangdong, LIU Yu, ZHANG Fengwu, LI Xuehu, XING Zhijun, LU Xihong, LIANG Jianping, FENG Jianqiang, ZHOU Xiang, WANG Zhen. Effects of ¹²C⁶⁺ Ion Beam Irradiation on the Quality of Tea Tree Essential Oil and Its Efficacy Against Foot-and-mouth Disease Virus[J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(2): 755-762.

参考文献

[1] 陶蕾,梁妍,周翔,等.碳离子束辐照处理对牛至精油微胶囊的缓释特性及抗鸡球虫病活性的影响[J].原子核物理评论,2018,35(2):179-188. TAO L,LIANG Y,ZHOU X,et al.Effect of carbon ion beam irradiation on sustained release characteristics and anti-coccidiosis activity of bovine to essential oil microcapsules[J].Nuclear Physics Review,2018,35(2):179-188.(in Chinese)
[2] 卫增泉.重离子束生物工程中的一些基本物理问题[J].激光生物学报,2003,5:321-325. WEI Z Q.Some basic physical matters in biotechnology with heavy ion beams[J].Acta Laser Biology Sinica,2003,5:321-325.(in Chinese)
[3] 王琳.重离子¹²C⁶⁺辐照促进拟南芥的生长发育及提高抗逆性的机理研究[D].郑州:郑州大学,2019. WANG L.Studies on the mechanisms of growth stimulation and enhanced stress tolerance induced by carbon ion beams irradiation in Arabidopsis thaliana[D].Zhengzhou:Zhengzhou University,2019.(in Chinese)
[4] 刘一可,隋丽,谢颖,等.100 MeV质子照射对肿瘤细胞线粒体氧化损伤及其作用机制研究[J].军事医学,2020,44(2):86-90. LIU Y,SUI L,XIE Y,et al.Mitochondrial oxidative damage and its mechanism in tumor cells induced by 100 MeV proton irradiation[J].Military Medicine,2020,44(2):86-90.(in Chinese)
[5] 梁剑平,卫增泉,张力,等.重离子辐射对喹乙醇的作用[J].辐射研究与辐射工艺学报,1999,17(1):24-27. LIANG J P,WEI Z Q,ZHANG L,et al.Effects of heavy ion radiation on quinethanol[J].Journal of Radiation Research and Radiation Technology,1999,17(1):24-27.(in Chinese)
[6] 许睿,韦松.20年来中药化学成分提取分离技术的进展[J].中成药,2006,11:1646-1651. XU R,WEI S.Advances in extraction and separation of chemical components of traditional Chinese medicine in 20 years[J].Proprietary Chinese Medicine,2006,11:1646-1651.(in Chinese)
[7] CARSON C F,HAMMER K A,RILEY T V.Melaleuca alternifolia (tea tree) oil:A review of antimicrobial and other medicinal properties[J].Clinical Microbiology Reviews,2006,19(1):50-62.
[8] 杨璐丹,谢曼英,余幸儿,等.茶树精油在多家医院重症监护室失禁性皮炎患者中的应用[J].中国医药科学,2018,3:107-109. YANG L D,XIE M Y,YU X E,et al.The application of tea tree essential oil in patients with incontinence dermatitis in intensive care unit of several hospitals[J].China Medicine and Pharmacy,2018,3:107-109.(in Chinese)
[9] 朱敏.基于茶油的抗衰老化妆品的研究开发[D].合肥:合肥工业大学,2017. ZHU M.Research and development of anti-aging cosmetics based on tea oil[D].Hefei:Hefei University of Technology,2017.(in Chinese)
[10] 黄德浩,吴伟超,吴国兴,等.茶树精油抗炎抗菌功效的研究现状[J].今日药学,2018,3:211-215. HUANG D H,WU W C,WU G X,et al.Anti-inflammatory and antibacterial effects of tea tree essential oil[J].Pharmacy Today,2018,3:211-215.(in Chinese)
[11] 尹伦甫.水产用抗寄生虫药研发新动态——植物精油杀虫活性因子的应用[J].北京水产,2007,6:13-14. YIN L F.Research and development of new dynamic-Plant essential oil insecticidal activity factor for aquatic antiparasitic drugs[J].Journal of Beijing Fisheries,2007,6:13-14.(in Chinese)
[12] RICCIONI L,ORZALI L.Activity of tea tree (Melaleuca alternifolia,Cheel)and thyme (Thymus vulgaris,Linnaeus.) essential oils against some pathogenic seed borne fungi[J].Journal of Essential Oil Research,2011,23(6):43-47.
[13] 孔庆娟.口蹄疫防控研究综述[J].湖南畜牧兽医,2018,6:43-45. KONG Q J.A Review of prevention and control of foot-and-mouth disease[J].Hunan Journal of Animal Science & Veterinary Medicine,2018,6:43-45.(in Chinese)
[14] 何随彬,金盈宇,龚志亮,等.口蹄疫疫苗最新研究进展[J].上海畜牧兽医通讯,2019,2:30-32. HE S B,JIN Y Y,GONG Z L,et al.Advances in foot-and-mouth disease vaccine[J].Shanghai Journal of Animal Husbandry and Veterinary Medicine,2019,2:30-32.(in Chinese)
[15] 孙涛,梁成珠,邓明俊,等.O型口蹄疫病毒核酸检测标准样品的制备[J].动物医学进展,2014,35(2):45-50. SUN T,LIANG C Z,DENG M J,et al.Preparation of O type FMDV reference materical used for nucleic acid detection[J].Progress in Veterinary Medicine,2014,35(2):45-50.(in Chinese)
[16] 刘娅丽,王志强,马丽娟,等.重离子辐照对人舌鳞癌CAL27细胞的生物学效应及其分子机制[J].肿瘤防治研究,2018,45(8):523-527. LIU Y L,WANG Z Q,MA L J,et al.Biological effect and molecular mechanism of heavy ion irradiation on human tongue squamous cell Carcinoma CAL27 cells[J].Cancer Research on Prevention and Treatment,2018,45(8):523-527.(in Chinese)
[17] 钟振声,樊丽妃,黄继兵.引种互叶白千层茶树油的化学成分及抑菌活性[J].华南理工大学学报(自然科学版),2011,39(1):53-57. ZHONG Z S,FAN L F,HUANG J B.Chemical components and antimicrobial activity of tea tree oil from introduced Melaleuca alternifolia[J].Journal of South China University of Technology (Natural Science Edition),2011,39(1):53-57.(in Chinese)
[18] 廖享,王青,符继红,等.近红外光谱法快速测定新疆薰衣草精油主要组分[J].光谱学与光谱分析,2015,35(9):2526-2529. LIAO H,WANG Q,FU J H,et al.Main components of Xinjiang lavender essential oil determined by partial least squares and near infrared spectroscopy[J].Spectroscopy and Spectral Analysis,2015, 35(9):2526-2529.(in Chinese)
[19] MIELKE S R,GARABED R.Environmental persistence of foot-and-mouth disease virus applied to endemic regions[J].Transboundary and Emerging Diseases,2020,67:543-554.
[20] RAHMAN A U,DHAMA K,ALI Q,et al.Foot and mouth disease in a wide range of wild hosts:A potential constraint in disease control efforts worldwide particularly in disease-endemic settings[J].Acta Tropica,2020,210:105567.
[21] ASHRAF S A,AL-SHAMMARI E,HUSSAIN T,et al.In vitro antimicrobial activity and identification of bioactive components using GC-MS of commercially available essential oils in Saudi Arabia[J].Food,Technology,2017,76(1):108-116.
[22] SCHNITZLER P,SCHÖN K,REICHLING J.Antiviral activity of australian tea tree oil and eucalyptus oil against herpes simplex virus in cell culture[J].Pharmazie,2001,56(4):343-347.
[23] CARSON C F,HAMMER K A,RILEY T V.Melaleuca alternifolia (tea tree) oil:A review of antimicrobial and other medicinal properties[J].Clinical Microbiology Reviews,2006,19(1):50-62.
[24] 程峰,尚若锋,杨珍,等.茶树精油抗微生物作用机理研究进展[J].食品工业科技,2020:1-10. CHENG F,SHANG R F,YANG Z,et al Advances in antimicrobial mechanism of tea tree oil[J].Science and Technology of Food Industry,2020:1-10.(in Chinese)
[25] ASTANI A,REICHLING J,SCHNITZLER P.Comparative study on the antiviral activity of selected monoterpenes derived from essential oils[J].Phytotherapy Research, 2009,24:673-679.
[26] GAROZZO A,TIMPANARO R,STIVALA A,et al.Activity of Melaleuca alternifolia (tea tree) oil on influenza virus A/PR/8:Study on the mechanism of action[J].Antiviral Research,2011,89(1):83-88.
[27] MILLAR B C,MOORE J E.Successful topical treatment of hand warts in a paediatric patient with tea tree oil (Melaleuca alternifolia)[J].Comple-mentary Therapies in Clinical Practice,2008,14(4):225-227.
[28] JOHANNES C,VAN DER WOUDEN R V D S.Interventions for cutaneous molluscum contagiosum:A summarised Cochrane review[J].Clinical & Experimental Dermatology,2011,36(7):828-829.

¹²C⁶⁺离子束辐照对茶树精油质量及其杀灭口蹄疫病毒效果的影响

Effects of ¹²C⁶⁺ Ion Beam Irradiation on the Quality of Tea Tree Essential Oil and Its Efficacy Against Foot-and-mouth Disease Virus

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 3

编辑推荐

Metrics

本文评价

[1]	杨苏珍, 孙亚宁, 刘运超, 柴书军, 张改平. 口蹄疫病毒感染与免疫鉴别诊断及免疫评价二联试纸的优化[J]. 中国畜牧兽医, 2022, 49(10): 3953-3962.
[2]	刘汉平. 猪O型口蹄疫病毒样颗粒的构建及鉴定[J]. 中国畜牧兽医, 2019, 46(11): 3350-3357.
[3]	李夏莹, 郑鹭飞, 张秀杰, 杨坤, 汪其怀, 周荣, 吴添文. 口蹄疫病毒研究进展[J]. , 2015, 42(7): 1910-1916.