长顺绿壳蛋鸡SLC8A1基因多态性及其对蛋壳品质的影响

doi:10.16431/j.cnki.1671-7236.2022.12.018

摘要/Abstract

摘要： 【目的】探究溶质载体家族8成员A1（solute carrier family 8 member A1，SLC8A1）基因多态性对长顺绿壳蛋鸡蛋壳品质的影响，为今后改善蛋壳品质提供参考。【方法】根据GenBank数据库中鸡SLC8A1基因序列（登录号：NC_052534.1），使用Primer Premier 3.0软件设计引物进行PCR扩增，采用直接测序法筛选SLC8A1基因SNP位点，并利用SPSS 22.0软件检测SNP位点不同基因型蛋壳指标的差异。【结果】在SLC8A1基因外显子11上共发现3个新的SNPs：g.16085681 T>C、g.16085766 C>T和g.16085781 T>C，共存在3种单倍型：H1（TCT）、H2（CTC）、H3（TTC），以及6种双倍型：H1H1（TTCCTT）、H1H2（TCTCTC）、H1H3（TTTCTC）、H2H2（CCTTCC）、H2H3（CTTTCC）、H3H3（TTTTCC）。χ²检验结果显示，3个SNPs位点均处于Hardy-Weinberg平衡状态（P>0.05），均表现为中度多态。关联性分析结果显示，g.16085681 T>C位点TC基因型个体蛋壳强度显著高于TT基因型（P<0.05），g.16085681 T>C位点和单倍型H2（C_16085681T_16085766C_16085781）对SLC8A1基因mRNA二级结构产生明显的影响；g.16085766 C>T位点CC基因型个体蛋形指数显著高于TT基因型（P<0.05）；双倍型H1H1、H1H3个体蛋形指数显著高于H2H3，H2H3个体蛋壳强度和蛋壳厚度均显著高于H1H3，H1H2、H2H2个体蛋重均显著高于H3H3（P<0.05）。【结论】g.16085681 T>C位点可作为影响蛋壳强度的标记位点，g.16085766 C>T位点可作为影响蛋形指数的标记位点，双倍型H2H3可能是蛋壳强度和蛋壳厚度的有利双倍型。

关键词: 长顺绿壳蛋鸡; SLC8A1基因; 多态性; 蛋壳品质; 关联分析

Abstract: 【Objective】 This study was aimed to explore the effect of solute carrier family 8 member A1 (SLC8A1) gene polymorphism on the eggshell quality in Changshun Blue-eggshell chickens, so as to provide reference for improving eggshell quality in the future.【Method】 The sequence of SLC8A1 gene in chicken was searched by GenBank database (accession No.:NC_052534.1), Primer Premier 3.0 software was used to design primers for PCR amplification, and the SNP of SLC8A1 gene was screened by direct sequencing.The differences of eggshell indexes of different genotypes of SNP were detected by SPSS 22.0 software.【Result】 Three new SNPs were identified in exon 11 of SLC8A1 gene:g.16085681 T>C, g.16085766 C>T and g.16085781 T>C.A total of 3 haplotypes and 6 diplotypes were detected, the haplotypes were H1(TCT), H2(CTC) and H3(TTC), and the diplotypes were H1H1(TTCCTT), H1H2(TCTCTC), H1H3(TTTCTC), H2H2(CCTTCC), H2H3(CTTTCC) and H3H3(TTTTCC).χ² test showed that three SNPs were in Hardy-Weinberg equilibrium (P>0.05), all showed moderate polymorphism.The results of correlation analysis showed that the eggshell strength of TC genotype individual of g.16085681 T>C was significantly higher than that of TT genotype (P<0.05).g.16085681 T>C and haplotype H2 (C_16085681T_16085766C_16085781) had significant effects on the secondary structure of SLC8A1 gene mRNA.The egg shape index of CC genotype individual of g.16085766 C>T was significantly higher than that of TT genotype (P<0.05).The egg shape index of H1H1 and H1H3 individuals were significantly higher than that of H2H3 (P<0.05).The eggshell strength and eggshell thickness of H2H3 individual were significantly higher than those of H1H3 (P<0.05).The egg weight of H1H2 and H2H2 individuals were significantly higher than that of H3H3 (P<0.05).【Conclusion】 g.16085681 T>C could be used a marker site affecting eggshell strength, g.16085766 C>T mutation could be used a marker site affecting egg shape index, and diplotype H2H3 might be a favorable diploid of eggshell strength and eggshell thickness.

Key words: Changshun Blue-eggshell chickens; SLC8A1 gene; polymorphism; eggshell quality; correlation analysis

中图分类号:

S831.2

王盈童, 杨酸, 谭元成, 王春源, 张依裕. 长顺绿壳蛋鸡SLC8A1基因多态性及其对蛋壳品质的影响[J]. 中国畜牧兽医, 2022, 49(12): 4688-4696.

WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.

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