中国美利奴羊eNOS基因exon8多态性及其与布鲁氏菌病易感性相关性分析

doi:10.16431/j.cnki.1671-7236.2015.12.027

›› 2015, Vol. 42 ›› Issue (12): 3294-3299.doi: 10.16431/j.cnki.1671-7236.2015.12.027

中国美利奴羊eNOS基因exon8多态性及其与布鲁氏菌病易感性相关性分析

许万云, 王会敏, 李建华, 汪文伦, 胡梦薇, 高剑峰

石河子大学生命科学学院, 石河子 832003

收稿日期:2015-05-06 出版日期:2015-12-20 发布日期:2015-12-30
通讯作者: 高剑峰 E-mail:jianfengg@shzu.edu.cn
作者简介:许万云(1991-),女,山东青岛人,硕士,研究方向:动物基因工程,E-mail:loveheavenyun@163.com
基金资助:
重要人兽共患胞内寄生菌病流行特征及病原致病机制研究(973计划)(2010CB530200)

The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility

XU Wan-yun, WANG Hui-min, LI Jian-hua, WANG Wen-lun, HU Meng-wei, GAO Jian-feng

College of Life Sciences, Shihezi University, Shihezi 832003, China

Received:2015-05-06 Online:2015-12-20 Published:2015-12-30

摘要/Abstract

摘要： 本试验旨在研究内皮型一氧化氮合酶(eNOS)基因exon8多态性与中国美利奴羊布鲁氏菌病易感性的相关性。利用生物信息学方法对人和中国美利奴羊eNOS基因exon8序列进行比对,并对NCBI SNP数据库上人eNOS基因exon8多态性进行了统计分析。通过PCR-SSCP对101只中国美利奴羊阴性样本和61只中国美利奴羊阳性样本eNOS基因exon8的多态性进行检测,然后对不同等位基因进行PCR产物测序,旨在确定该基因exon8多态性位点,并对SNP位点的等位基因频率、基因型频率进行统计分析。在eNOS基因exon8序列142 bp处检测到一个新的SNP位点(ss974768653:A142G),该位点在病例组和对照组之间的等位基因频率及各基因型间不存在显著差异性(P >0.05)。eNOS基因exon8 A142G多态性位点与中国美利奴羊布鲁氏菌病易感性可能无相关性。

关键词: 绵羊; eNOS; PCR-SSCP; 多态性; 外显子

Abstract: The association of Chinese Merino sheep eNOS gene exon8 polymorphisms with brucellosis susceptibility was studied in this research.The sequences of human and Chinese Merino sheep eNOS gene exon8 were aligned by the bioinformatics methods,and the polymorphisms of human eNOS gene in the NCBI SNP database were statistically analyzed.The polymorphisms of 101 Chinese Merino sheep negative samples and 61 Chinese Merino sheep positive samples were detected by PCR-SSCP method,and then the PCR products of different alleles were sequenced,aiming to determine exon8's polymorphism loci,and the allele frequency and genotype frequency of SNP loci were statistically analyzed.A novel SNP locus (ss974768653:A142G) was detected at the 142 bp of eNOS gene exon8,and the locus had no difference in allele frequency and each genotype between negative and positive groups (P >0.05).There might be no correlation between the A142G polymorphism locus of Chinese Merino sheep eNOS gene exon8 and brucellosis susceptibility.

Key words: sheep; eNOS; PCR-SSCP; polymorphism; exon

中图分类号:

Q754

许万云, 王会敏, 李建华, 汪文伦, 胡梦薇, 高剑峰. 中国美利奴羊eNOS基因exon8多态性及其与布鲁氏菌病易感性相关性分析[J]. , 2015, 42(12): 3294-3299.

XU Wan-yun, WANG Hui-min, LI Jian-hua, WANG Wen-lun, HU Meng-wei, GAO Jian-feng. The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility[J]. , 2015, 42(12): 3294-3299.

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中国美利奴羊eNOS基因exon8多态性及其与布鲁氏菌病易感性相关性分析

The Polymorphisms of Chinese Merino Sheep eNOS Gene exon8 and its Association with Brucellosis Susceptibility

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