《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (3): 629-637.doi: 10.11843/j.issn.1671-7236.2015.03.019

• 遗传繁育 • 上一篇    下一篇

肌细胞生成素基因多态性与泸宁鸡生长性状的关联研究

刘红丽1, 徐亚欧1, 熊斌2, 胡卫民1, 梅寒1, 石浩1, 左斌1, 聂晓庆1, 王海1   

  1. 1. 西南民族大学生命科学与技术学院, 成都 610041;
    2. 冕宁县畜牧局, 凉山 615600
  • 收稿日期:2014-10-17 出版日期:2015-03-20 发布日期:2015-03-30
  • 通讯作者: 徐亚欧 E-mail:xuyaou@163.com
  • 作者简介:刘红丽(1988-),女,河南商丘人,硕士生,研究方向:家禽育种,E-mail:ruolitel@163.com
  • 基金资助:

    四川省畜禽育种攻关项目(2011NZ0099-6);四川应用基础项目(2013JY0044);西南民族大学项目(2012NFW001);西南民族大学研究生"创新型科研项目"(CX2014SZ110)

Study on the Correlation between Polymorphisms of MyoG Gene and Growth Traits in Luning Chicken

LIU Hong-li1, XU Ya-ou1, XIONG Bin2, HU Wei-min1, MEI Han1, SHI Hao1, ZUO Bin1, NIE Xiao-qing1, WANG Hai1   

  1. 1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
    2. Mianning Animal Husbandry Bureau, Liangshan 615600, China
  • Received:2014-10-17 Online:2015-03-20 Published:2015-03-30

摘要: 本研究旨在探讨肌细胞生成素(myogenin,MyoG)基因多态性与泸宁鸡生长性状的关联性.采用PCR-SSCP和DNA测序技术,对泸宁鸡MyoG基因5'调控区的多态性进行检测.结果表明,泸宁鸡MyoG基因5'调控区存在4个多态位点(A153G、A267G、A446G和C612T).在153 bp处存在A/G突变,形成3种基因型(AA、AG和GG),其中AA基因型肝脏重显著高于AG、GG基因型(P <0.05).在446 bp处存在的A/G突变,形成3种基因型(AA、AG和GG),该位点GG基因型屠宰率显著高于AA、AG基因型(P <0.05),AG、GG基因型胸肌率显著高于AA基因型(P <0.05).以上结果显示,MyoG基因5'调控区的多态性与泸宁鸡肝脏重、屠宰率、胸肌率有一定的关联性,可作为泸宁鸡相应生长性状的候选基因.

关键词: 泸宁鸡; MyoG基因; PCR-SSCP; 生长性状; 关联分析

Abstract: This study was designed to investigate the correlation between SNP of MyoG gene and growth traits in Luning chicken.The 5'polymorphism of MyoG gene were detected by PCR-SSCP and DNA sequencing technology.As a result,four SNPs (A153G,A267G,A446G and C612T) were found in MyoG gene.Analysis showed that the mutation in 153 bp of MyoG gene exhibited three genotypes (AA,AG and GG),individuals with AA genotype had significant effect on liver weight than those with AG and GG genotypes (P <0.05).The mutation in 446 bp of MyoG gene performed three genotypes (AA,AG and GG),compared with individuals with GG genotype,those with AA and AG genotypes had significant effect on slaughter percentage (P <0.05),and with AA Genotype,those with AG and GG genotypes had significant effect on breast muscle percentage (P <0.05).It could be stated that MyoG gene showed a significant effect on Luning chicken growth traits.So MyoG gene could be served as a candidate gene in growth traits of Luning chicken for further study.

Key words: Luning chicken; MyoG gene; PCR-SSCP; growth traits; association analysis

中图分类号: