不同精粗比柠条饲粮对湖羊生长性能、肉品质及肠道微生物区系的影响

doi:10.16431/j.cnki.1671-7236.2023.09.018

摘要/Abstract

摘要： 【目的】研究不同精粗比柠条饲粮对湖羊生长性能、肉品质及肠道微生物区系的影响。【方法】试验选取2月龄左右、体重一致(15.36 kg±3.36 kg)断奶湖羊60只,平均分为3组,每组5个重复,每个重复4只羊。粗饲料组成为60％柠条、20％花生秧及20％苜蓿,3组粗饲料与精料比例分别为60∶40、50∶50、40∶60。试验预饲期10 d,正试期60 d,试验期内每隔15 d记录各组湖羊的体重;试验结束后第2天屠宰,采集3组湖羊背最长肌、股三头肌和空肠内容物,进行肉品质分析和肠道菌群检测。【结果】3组湖羊体重在15、30和45 d无显著差异(P＞0.05)。试验第60天时,60∶40组湖羊平均体重和平均日增重显著高于40∶60组(P＜0.05);50∶50组股三头肌总氨基酸含量最高(P＜0.05),各种风味氨基酸含量也较高;各组湖羊背最长肌和股三头肌各种氨基酸组成及含量均无显著差异(P>0.05);湖羊肌肉中各脂肪酸含量及比例在3组间均无显著差异(P＞0.05)。各组湖羊肠道微生物门水平主要细菌组成为厚壁菌门(Firmicutes)、放线菌门(Actinobacteria)和髌杆菌门(Patescibacteria),其中厚壁菌门、变形菌门(Proteobacteia)和软壁菌门(Tenericutes)菌群丰度随饲粮中粗饲料的降低而降低(P＜0.05);在属水平上,双歧杆菌科菌属(Aeriscardovia)丰度随粗饲料比例降低有升高的趋势(0.05<P＜0.1)。【结论】柠条粗饲料与精料比例在60∶40时湖羊生长性能最高;50∶50时羊肉品质更好。饲喂不同精粗比柠条饲粮一定程度上影响肠道微生物结构。

关键词: 柠条; 湖羊; 肠道微生物

Abstract: 【Objective】 This experiment was conducted to study the effects of different concentrate to forage ratio of Caragana diet on growth performance,meat quality and intestinal microflora of Hu sheep.【Method】 60 Hu sheep about 2 months of age with uniform weight (15.36 kg±3.36 kg) were divided equally into three groups,there were 5 replicates per group,4 sheep per replicate,with 60％ caragana hay,20％ peanut hay and 20％ alfalfa hay as the roughage diets,and the ratios of roughage to concentrate in the three groups were 60∶40,50∶50 and 40∶60,respectively.The adaption period was 10 days,and the experiment period was 60 days. The body weight of each group was recorded every 15 days during the test period,and the slaughter test was conducted on the second day after the end of the test period.Longissimus dorsal muscle and triceps femoris samples, and jejunum contents of Hu sheep were collected for meat quality analysis and intestinal flora testing.【Result】 The results showed that there was no significant difference in body weight among the three groups at 15,30 and 45 days (P＞0.05).On the 60th day of the experiment,the average body weight and average daily gain of the 60∶40 group were significantly higher than those of the 40∶60 group (P＜0.05).The total amino acid content of triceps femoris in the 50∶50 group was the highest (P＜0.05), and flavor amino acids contents in which were higher too. There was no significant change in the content and proportion of fatty acids in the muscle of Hu sheep among the three groups (P＞0.05).The main bacterial composition of the gut microbiota phylum level in three group of Hu sheep was Firmicutes,Actinobacteria and Patescibacteria.The abundance of Firmicutes,Proteobacteia and Tenericutes decreased with the decrease of roughage (P＜0.05).At the genus level,Aeriscardovia abundance tended to increase with roughage reduction (0.05<P＜0.1).【Conclusion】 When the ratio of roughage to concentrate was 60∶40,the growth performance of Hu sheep was the highest,and the mutton quality was better at 50∶50.Feeding different ratio of roughage to concentrate affected the intestinal microbial structure to some extent.

Key words: Caragana; Hu sheep; intestinal microbiota

中图分类号:

S816.5

张瑞, 王强, 张磊, 肖宇航, 薛云, 孙耀军, 崔久增, 宋宇轩. 不同精粗比柠条饲粮对湖羊生长性能、肉品质及肠道微生物区系的影响[J]. 中国畜牧兽医, 2023, 50(9): 3622-3629.

ZHANG Rui, WANG Qiang, ZHANG Lei, XIAO Yuhang, XUE Yun, SUN Yaojun, CUI Jiuzeng, SONG Yuxuan. Effects of Caragana Diet with Different Concentrate to Forage Ratio on Growth Performance,Meat Quality and Intestinal Microflora of Hu Sheep[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(9): 3622-3629.

参考文献

[1] JIANG M,CHEN H M,HE S B,et al.Sequencing,characterization,and comparative analyses of the plastome of Caragana rosea var.rosea[J].International Journal of Molecular Sciences,2018,19(5):1419.
[2] MA C C,GAO Y B,GUO H Y,et al.Physiological adaptations of four dominant Caragana species in the desert region of the Inner Mongolia plateau[J].Journal of Arid Environments,2007,72(3):247-254.
[3] 罗惠娣,牛西午,毛杨毅,等.柠条饲喂山羊效果试验[J].草业科学,2006,23(4):63-66. LUO H D,WU X W,MAO Y Y,et al.Goat feeding experiment for Korshinsk Peashrub[J].Pratacultural Science,2006,23(4):63-66.(in Chinese)
[4] 张平,黄应祥,王珍喜.采用不同方法加工的柠条饲喂育肥羊效果的研究[J].中国畜牧兽医,2004,31(11):7-9. ZHANG P,HUANG Y X,WANG Z X.Effect of Caragana processed by different methods on feeding fattening sheep[J].China Animal Husbandry & Veterinary Medicine,2004,31(11):7-9.(in Chinese)
[5] 葛翠翠,李昊,冯帆,等.柠条利用方式对宁夏育肥滩羊体外消化及发酵参数的影响[J].江苏农业科学,2019,47(6):144-147. GE C C,LI H,FEN F,et al.Effect of Caragana utilization styles on in vitro digrstion and fermentation parameters of fattening Ningxia Tan sheep[J].Jiangsu Agricultural Sciences,2019,47(6):144-147(in Chinese)
[6] 姬天龙.柠条不同处理营养成分与饲喂效果研究[J].现代农业科技,2020,22:183-184. JI T L.Study on nutrient composition and feeding effect of Caragana under different treatments [J].Modern Agricultural Science and Technology,2020,22:183-184.(in Chinese)
[7] 冯旭东,段开红,郜晋楠.柠条叶中单宁的提取工艺的优化[J].饲料研究,2019,42(10):82-85. FEN X D,DUAN K H,GAO J N.Optimization of extraction about leaves of Caragana[J].Feed Research,2019,42(10):82-85.(in Chinese)
[8] 罗惠娣,毛杨毅,牛西午,等.柠条喂羊消化试验[J].中国草食动物,2003,S1:104-106. LUO H D,MAO Y Y,NIU X W,et al.Digestion experiment of Caragana feeding sheep[J].China Herbivore Science,2003,S1:104-106.(in Chinese)
[9] 马吉锋,杨宇为,于洋,等.柠条与玉米芯添加比例对西杂牛增重和血液生化指标的影响研究[J].饲料工业,2020,41(5):56-60. MA J F,YANG Y W,YU Y,et al.Effect of Caragana and corncob on weight gain and blood biochemical paraments in Simmental hybird cattle[J].Feed Industry,2020,41(5):56-60.(in Chinese)
[10] 智成英,马湘丽,屈海军.柠条配合饲料育肥羊效果观察[J].当代畜禽养殖业,2011,5:50-51. ZHI C Y,MA X L,QU H J.Effect of Caragana compound feed on fattening sheep [J].Modern Animal Husbandry,2011,5:50-51.(in Chinese)
[11] 马吉锋,张俊丽,于洋,等.柠条不同添加比例颗粒日粮对滩羊生产性能影响的研究[J].宁夏农林科技,2019,60(9):69-72. MA J F,ZAHNG J L,YU Y,et al.Effects of different proportions of Caragana granule diet on the performance of Tan sheep[J].Journal of Ningxia Agriculture and Forestry Science and Technology,2019,60(9):69-72.(in Chinese)
[12] 卢盛勇,陈胜昌,陈祥,等.不同精粗比日粮对贵州白山羊生长性能、屠宰性能及肉品质的影响[J].中国畜牧杂志,2021,57(6):219-222. LU S Y,CHEN S C,CHEN X,et al.Effects of different concentrate to forage ratios on growth performance,slaughter performance and meat quality of Guizhou White goats[J].Chinese Journal of Animal Science,2021,57(6):219-222.(in Chinese)
[13] 李晓燕,王建军,严秉莲,等.不同精粗比TMR与全价颗粒料对湖羊生长性能、血液生化指标和屠宰性能的影响[J].中国草食动物科学,2020,40(6):28-31. LI X Y,WANG J J,YAN B L,et al.Effects of different fine ratio TMR and full price granular meterial on growth performance,blood biochemical index and slaughter peformance of Hu sheep[J].China Herbivore Science,2020,40(6):28-31.(in Chinese)
[14] 陈雪,梁克红,王靖,等.膳食中多不饱和脂肪酸对心血管疾病防治研究进展[J].中国油脂,2020,45(10):87-94. CHEN X,LIANG K H,WANG J,et al.Progress in prevention and treatment of cardiovascular diseases by dietary polyunsaturated fatty acids[J].China Oils and Fats,2020,45(10):87-94.(in Chinese)
[15] WHO/FAO/UNU.Protein and amino acid requirements in human nutrition report of a joint WHO/FAO/UNU expert consultation[R].Geneva:World Health Organization,2007.
[16] 卢盛勇,陈胜昌,李景上,等.不同精粗比饲粮对贵州白山羊肌肉营养组成的影响[J].动物营养学报,2021,33(6):3380-3388. LU S Y,CHEN S C,LI J S,et al.Effects of different concentrate to roughage ratios on muscle nutritional composition of Guizhou White goat[J].Chinese Journal of Animal Nutrition,2021,33(6):3380-3388.(in Chinese)
[17] 杜梅,梁泽毅,张剑搏,等.反刍动物肠道菌群与宿主肠道黏膜免疫互作及其调控研究进展[J].动物营养学报,2021,33(5):2483-2494. DU M,LIANG Z Y,ZHANG J B,et al.Recent advance on interaction between intestinal microflora and host mucosal immune system of ruminants[J].Chinese Journal of Animal Nutrition,2021,33(5):2483-2494.(in Chinese)
[18] HONGLADA T,WUTTICHAI M,THANAPORN L,et al.Comparative analysis of microbial profiles in cow rumen fed with different dietary fiber by tagged 16S rRNA gene pyrosequencing[J].Current Microbiology,2013,67(2):130-137.
[19] SPENCE C,WELLS W G, SMITH C J,et al.Characterization of the primary starch utilization operon in the obligate anaerobe Bacteroides fragilis:Regulation by carbon source and oxygen[J].Journal of Bacteriology,2006,188(13):4663-4672.
[20] 程新东,辛国省,伍修锟,等.反刍动物肠道菌群对饲料利用效率调节效应的研究进展[J].动物营养学报,2021,33(5):2474-2482. CHENG X D,XIN G S,WU X K,et al.Research progress on effects of gut microbiota on regulation of feed efficiency in ruminant[J].Chinese Journal of Animal Nutrition,2021,33(5):2474-2482.(in Chinese)
[21] 占今舜,杨群,胡耀,等.日粮精粗比对湖羊瘤胃发酵和菌群结构的影响[J].草业学报,2020,29(7):122-130. ZHAN J S,YANG Q,HU Y,et al.Effects of dietary concentration:Roughage ratio on rumen fermentation and flora population structure in Hu sheep[J].Acta Prataculturae Sinica,2020,29(7):122-130.(in Chinese)
[22] RAABIS S,LI W,CERSOSIMO L.Effects and immune responses of probiotic treatment in ruminants[J].Veterinary Immunology and Immunopathology,2019,208:58-66.