›› 2010, Vol. 37 ›› Issue (10): 174-177.

• 疾病防治 • 上一篇    下一篇

不同中药复方对3种鸡细菌性腹泻病原菌的体外抑菌试验

张丁华1，2， 王艳丰1， 许兰菊2， 朱金凤1， 尹凤阁2   

  1. （1. 河南农业职业学院动物科学系， 中牟 451450；2.河南农业大学牧医工程学院， 郑州 450002）
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-10-20 发布日期:2010-10-20
  • 通讯作者: 王艳丰

Bacteriostasis Test in vitro of Different Traditional Chinese Medicines to Three Kinds of Pathogenic Bacteria Causing Chicken Bacterial Diarrhea

ZHANG Ding-hua1，2，WANG Yan-feng1，XU Lan-ju2 ，ZHU Jin-feng1，YIN Feng-ge2   

  1. （1． Department of Animal Science, Henan Agricultural Vocational College, Zhongmu 451450, China; 2. College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China）
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-10-20 Published:2010-10-20
  • Contact: WANG Yan-feng

摘要： 为研究中药复方对3种鸡细菌性腹泻病原菌的抑制效果，采用纸片法对自拟的6个中药复方进行敏感性试验，并用试管二倍法测定中药复方的最小抑菌浓度。结果表明，6个中药复方均具有不同程度的抑菌作用，其中复方4对大肠杆菌的抑菌效果较好，平均抑菌圈直径为21.2 mm，平均MIC值为62.5 mg/mL；复方1对沙门氏菌效果较好，平均抑菌圈直径为19.8 mm，平均MIC值为41.7 mg/mL；复方3对多杀性巴氏杆菌效果较好，平均抑菌圈直径为23.3 mm，平均MIC值为3.92 mg/mL，3个复方均呈高度敏感。表明中药复方可以用于鸡细菌性腹泻的防治。

关键词: 中药复方; 鸡; 细菌性腹泻; 体外抑菌

Abstract: In order to study the bacteriostatic effect of traditional Chinese medicines on three kinds of pathogenic bacteria causing chicken bacterial diarrhea, paper strip method was applied to carry out sensitive tests of 6 self-made traditional Chinese medicines, and the tube dilution method was taken to test the minimal inhibitory concentration of the traditional Chinese medicines. Results showed that the 6 traditional Chinese medicines have varying degrees of bacteriostasis. Among the 6 traditional Chinese medicines, Compound 4 had better bacteriostasis on Escherichiacoli, the average bacteriostasis circle diameter was 21.2 mm, and the average MIC value was 62.5 mg/mL; compound 1 had better bacteriostasis on Salmonella bacillus, the average bacteriostasis circle diameter was 19.8 mm, and the average MIC value was 41.7 mg/mL; compound 3 had better bacteriostasis on Pasteurella multocida, the average bacteriostasis circle diameter was 23.3 mm, and the average MIC value was 3.92 mg/mL. The three compounds assumed high sensitivity indicated that the traditional Chinese medicines could be used in the prevention and treatment of chicken bacterial diarrhea.

Key words: traditional Chinese medicines; chicken; bacterial diarrhea; bacteriostasis test in vitro

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