织金白鹅PPP3CA基因多态性及其与体尺性状的关联分析

doi:10.16431/j.cnki.1671-7236.2025.04.025

Abstract

Abstract: 【Objective】 The aim of this study was to identify the single nucleotide polymorphism (SNPs) loci of protein phosphatase 3 catalytic subunit α (PPP3CA) of Zhijin White geese,and explore the effect of PPP3CA gene polymorphism on body size traits.【Method】 The PPP3CA gene SNPs of 144 Zhijin White geese were identified by PCR amplification and Sanger direct sequencing.The gene frequency,genotype frequency,number of effective allele (Ne) and polymorphism information content (PIC) were calculated using Excel 2010 software,and the Hardy-Weinberg equilibrium state was analyzed.The association between SNPs and body size traits of 144 Zhijin White geese were analyzed by One-Way analysis of variance in the general linear model (GLM) of SPSS 25.0 software.【Result】 9 SNPs were found in intron 2 and 4 of PPP3CA gene in Zhijin White geese,namely g.58398 A＞G,g.58504 A＞G,g.58541 A＞G,g.68997 A＞G,g.69001 C＞T,g.69034 C＞T,g.69052 T＞C,g.69070 T＞C,and g.69114 C＞T,respectively.Except for g.69034 C＞T,which was a low polymorphic site (PIC＜0.25),the other SNPs were moderate polymorphic loci (0.25＜PIC＜0.5).The Chi-square test results showed that the SNPs g.58398A ＞G,g.69001 C＞T,g.69070 T＞C and g.69114 C＞T were consistent with Hardy-Weinberg equilibrium (P＞0.05).However,the SNPs g.58504 A＞G,g.58541 A＞G,g.68997 A＞G,g.69034 C＞T and g.69052 T＞C deviated from Hardy-Weinberg equilibrium (P＜0.05).The results of association analysis showed that in the g.58504 A＞G and g.58541 A＞G,the pelvic width of GG genotype individuals were extremely significant or significantly higher than that of AA genotype (P＜0.01 or P＜0.05).【Conclusion】 There were 9 SNPs in PPP3CA gene of Zhijin White geese,among which g.58504 A＞G and g.58541 A＞G were correlated with pelvic width to different degrees,indicating that these SNPs could be used as reference loci for molecular marker-assisted breeding of Zhijin White geese,laying a theoretical foundation for further study of PPP3CA gene in geese.

Key words: Zhijin White geese; PPP3CAgene; SNPs; body size traits; association analysis

CLC Number:

S835

ZHAO Zhonglong, ZHANG Yong, YANG Hong, YANG Runqian, AI Zhaobi, YE Li, SHU Chang. Analysis of PPP3CA Gene Polymorphism and Its Association with Body Size Traits in Zhijin White Geese[J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(4): 1729-1738.

References

[1] ZHAO Z,YANG H,WANG Z,et al.Metabolomics analysis of the yolk of Zhijin White goose during the embryogenesis based on LC-MS/MS[J].PLoS One,2024,19(2):e0297429.
[2] 王清峰,陈胜昌,乔艳龙,等.1~4周龄织金白鹅能量和粗蛋白需要量研究[J].中国家禽,2017,39(2):60-63.WANG Q F,CHEN S C,QIAO Y L,et al.Study on energy and crude protein requirements of Zhijin White geese aged from 1 to 4 weeks[J].China Poultry,2017,39(2):60-63.(in Chinese)
[3] 王凯.神经钙调蛋白磷酸酶PPP3CA特异性siRNA对VEGF和FGF2诱导的羊胎盘动脉血管内皮细胞殖的影响[D].大连:大连医科大学,2008.WANG K.Effect of PPP3CA siRNA on VEGF-and FGF2-stimulated cell proliferation in ovine fetoplacental artery endothelial cells[D].Dalian:Dalian Medical University,2008.(in Chinese)
[4] 谷婧,田利军.钙调神经磷酸酶在运动中的作用及调节[J].山西大同大学学报(自然科学版),2011,27(1):55-57.GU J,TIAN L J.Role and regulation of calcineurin in exercise[J].Journal of Shanxi Datong University (Natural Science Edition),2011,27(1):55-57.(in Chinese)
[5] HUDSON M B,WOODWORTH-HOBBS M E,ZHENG B,et al.miR-23a is decreased during muscle atrophy by a mechanism that includes calcineurin signaling and exosome-mediated export[J].American Journal of Physiology-Cell Physiology,2014,306(6):C551-C558.
[6] HUDSON M B,PRICE S R.Calcineurin:A poorly understood regulator of muscle mass[J].International Journal of Biochemistry&Cell Biology,2013,45(10):2173-2178.
[7] 刘卫军,沈瑛,丁健.蛋白磷酸酶2A的结构、功能和活性调节[J].生物化学与生物物理学报,2003,2:105-112.LIU W J,SHEN Y,DING J.Protein phosphatase 2A:Its structure,function and activity regulation[J].Acta Biochimica et Biophysica Sinica,2003,2:105-112.(in Chinese)
[8] COHEN P.The structure and regulation of protein phosphatases[J].Annual Review of Biochemistry,1989,58:453-508.
[9] BAR L,GROSSMANN C,GEKLE M,et al.Calcineurin inhibitors regulate fibroblast growth factor 23(FGF23) synthesis[J].Naunyn-Schmiedebergs Archives of Pharmacology,2017,390(11):1117-1123.
[10] WAN L,MA J,XU G,et al.Molecular cloning,structural analysis and tissue expression of protein phosphatase 3 catalytic subunit alpha isoform (PPP3CA) gene in Tianfu goat muscle[J].International Journal of Molecular Sciences,2014,15(2):2346-2358.
[11] SHAO F,LIU J,REN M,et al.Identification of loci controlling the dwarfism trait in the White Sailfin Molly (Poecilia latipinna) using genome-wide association studies based on genotyping-by-sequencing[J].Genes (Basel),2019,10(6):418.
[12] DIAS M M,SOUZA F R,TAKADA L,et al.Study of lipid metabolism-related genes as candidate genes of sexual precocity in Nellore cattle[J].Genetics and Molecular Research,2015,14(1):234-243.
[13] ISLAM R,LIU X,GEBRESELASSIE G,et al.Genome-wide association analysis reveals the genetic locus for high reproduction trait in Chinese Arbas cashmere goat[J].Genes Genomics,2020,42(8):893-899.
[14] REDGROVE K A,BERNSTEIN I R,PYE V J,et al.Dynamin 2 is essential for mammalian spermatogenesis[J].Scientific Reports,2016,6:35084.
[15] REID A T,LORD T,STANGER S J,et al.Dynamin regulates specific membrane fusion events necessary for acrosomal exocytosis in mouse spermatozoa[J]. Journal of Biological Chemistry,2012,287(45):37659-37672.
[16] GUO S,LIU Y,XU Y,et al.Identification of key genes affecting sperm motility in chicken based on whole-transcriptome sequencing[J].Poultry Science,2023,102(12):103135.
[17] 秦宁,徐日福,王志贤,等.钙调磷酸酶在肌纤维类型转化上的研究进展[J].中国畜牧兽医,2011,38(9):34-36.QIN N,XU R F,WANG Z X,et al.Research progress of calcineurin in the transformation of muscle fiber type[J].China Animal Husbandry&Veterinary Medicine,2011,38(9):34-36.(in Chinese)
[18] SHU J T,QIN X,SHAN Y J,et al.Oxidative and glycolytic skeletal muscles show marked differences in gene expression profile in Chinese Qingyuan partridge chickens[J].PLoS One,2017,12(8):e0183118.
[19] TAN G H,LI J Z,ZHANG Y Y,et al.Association of PRKCA expression and polymorphisms with layer duck eggshell quality[J].British Poultry Science,2021,62(1):8-16.
[20] 薛倩,王金玉,张跟喜,等.黑素皮质素受体3基因(MC3R)多态性及其单倍型组合与京海黄鸡屠体性状的关联分析[J].农业生物技术学报,2015,23(3):344-351.XUE Q,WANG J Y,ZHANG G X,et al.Correlation analysis of melanocortin receptor 3 gene (MC3R) polymorphism and its haplotype combination with carcass traits of Jinghai Yellow chickens[J].Journal of Agricultural Biotechnology,2015,23(3):344-351.(in Chinese)
[21] LIU M,LI M,WANG S,et al.Association analysis of bovine Foxa2 gene single sequence variant and haplotype combinations with growth traits in Chinese cattle[J].Gene,2014,536(2):385-392.
[22] WANG G,LI M,ZHOU J,et al.A novel A>G polymorphism in the intron 2 of TBX3 gene is significantly associated with body size in donkeys[J].Gene,2021,785:145602.
[23] XU J,RUAN Y,SUN J,et al.Association analysis of PRKAA2 and MSMB polymorphisms and growth traits of Xiangsu hybrid pigs[J].Genes (Basel),2022,14(1):113.
[24] 王春源,杨酸,谭元成,等.PPARα基因SNPs对关岭黄牛生长性状的影响[J].中国畜牧杂志,2023,59(11):138-143.WANG C Y,YANG S,TAN Y C,et al.Effects of PPARα gene SNPs on growth traits of Guanling Yellow cattle[J].Chinese Journal of Animal Science,2023,59(11):138-143.(in Chinese)
[25] WANG K,SONG Y,CHEN D,et al. Protein phosphatase 3 differentially modulates vascular endothelial growth factor-and fibroblast growth factor 2-stimulated cell proliferation and signaling in ovine fetoplacental artery endothelial cells[J]. Biology of Reproduction,2008,79(4):704-710.
[26] SHEN X,ZHAO X,HE H,et al.Evolutionary conserved circular MEF2A RNAs regulate myogenic differentiation and skeletal muscle development[J].PLoS Genetics,2023,9(9):e1010923.
[27] 陈祥,丁玫,许厚强,等.贵州5个地方猪品种微卫星标记的遗传多样性[J].中国畜牧杂志,2015,51(7):1-5.CHEN X,DING M,XU H Q,et al.Genetic diversity of micro-satellite markers in five local pig breeds in Guizhou[J].Chinese Journal of Animal Science,2015,51(7):1-5.(in Chinese)
[28] 郭徵力,赵中龙,杨红,等.织金白鹅微卫星标记遗传多样性及其与体尺指标的关联分析[J].农业生物技术学报,2023,31(11):2341-2357.GUO Z L,ZHAO Z L,YANG H,et al.Genetic diversity of Zhijin White goose using micro-satellite markers and its association with body size index[J].Journal of Agricultural Biotechnology,2023,31(11):2341-2357.(in Chinese)
[29] 梁宗琦,韩燕峰,曾桂萍,等.单倍型及其在菌物学研究中的应用[J].菌物学报,2020,39(2):223-237.LIANG Z Q,HAN Y F,ZENG G P,et al.Haplotype and its application in fungal research[J].Mycosystema,2020,39(2):223-237.(in Chinese)
[30] 王兴群,毛以智,张继,等.PPP3CA基因多态性与江口萝卜猪肉质性能关联性分析[J].中国畜牧杂志,2021,57(1):63-71.WANG X Q,MAO Y Z,ZHANG J,et al.Correlation analysis of PPP3CA gene polymorphism with quality and performance of Jiangkou Luobo pigs[J].Chinese Journal of Animal Science,2021,57(1):63-71.(in Chinese)
[31] 包艳波.大骨鸡生长发育规律研究及PPP3CA基因SNPs与肉质性状和产蛋性状的关联分析[D].长春:吉林农业大学,2012.BAO Y B.Study on law of growth and development of Dagu chicken and PPP3CA gene SNPs and correlation analysis of meat quality traits and egg production traits[D].Changchun:Jilin Agricultural University,2012.(in Chinese)
[32] 周迪,蒋桂荣,欧仁,等.赤水乌骨鸡PPP3CA基因多态性与生长性状关联分析[J].西南农业学报,2023,36(7):1547-1554.ZHOU D,JIANG G R,OU R,et al.Association analysis of PPP3CA gene polymorphism and growth traits in Chishui Black-bone silky fowl[J].Southwest Chinese Journal of Agricultural Sciences,2023,36(7):1547-1554.(in Chinese)
[33] 黄利华,张杉杉,鲍秀瑜,等.黑羽番鸭体尺和体质量与产蛋数相关性分析[J].西北农业学报,2020,29(4):496-501.HUANG L H,ZHANG S S,BAO X Y,et al.Correlation analysis of body size,body mass and egg production of Black Muscovy duck[J].Acta Agriculturae Boreali-occidentalis Sinica,2020,29(4):496-501.(in Chinese)

Analysis of PPP3CA Gene Polymorphism and Its Association with Body Size Traits in Zhijin White Geese

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	YANG Jinlin, LI Hui, ZHAO Depeng, SHI Yushi, LONG Xia, TAN Qisong. Polymorphisms of EDNRB2 Gene and Its Association with Skin Color Traits in Chishui Black-bone Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2025, 52(2): 759-770.
[2]	ZHOU Guangxian, TAN Jiayong, YANG Jian, LIU Yanfen, GAN Shangquan, ZHAO Zhihui, KANG Danju. Polymorphism of TBX15 Gene and Its Association Analysis with Body Size Traits in Leizhou Goats [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(9): 3930-3938.
[3]	XIANG Jin, YANG Qixin, LUO Hualun, HONG Yating, GUO Xiaojiang, YANG Hongwen, LI Wei, WANG Chunyuan, WU Yan, ZHANG Yiyu. Polymorphism of MMP2 Gene and Its Association with Reproductive Traits in Kele Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(3): 1142-1150.
[4]	BI Yazhen, SHANG Mingyu, XIONG Jinke, HU Wenping, HE Jianning, ZHANG Li. Polymorphism of SRP68 Gene and Its Association with Growth Traits in Hu Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(8): 3189-3198.
[5]	ZHANG Fangwei, ZHANG Qi, LEI Liangliang, SUN Wusheng, ZHANG Di, ZHANG Yunpeng, ZHANG Jingbo, WANG Xiuquan, ZHANG Jing, ZHANG Shumin. Polymorphism of HBEGF Gene and Its Association with Reproductive Traits in Songliao Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(6): 2370-2379.
[6]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[7]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[8]	FENG Xiaopin, LI Yinxia, ZHANG Chenjian, ZHANG Jun, MENG Chunhua, QIAN Yong, CAO Shaoxian. Coning and Polymorphism Identification of PLAG1 Gene and Its Association Analysis with Birth Weight and Body Size Traits in Boer Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3465-3474.
[9]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[10]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[11]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[12]	QIANG Hao, LIANG Peng, MENG Ke, RONG Xuan, FENG Dengzhen. Polymorphism of IGF1R Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1765-1785.
[13]	DENG Yihua, WANG Tianjiao, WANG Hongliang, DONG Yimeng, LIU Xin, XING Xiumei. Screening of Specific SNP Sites in Tahe Red Deer Based on Resequencing Technology [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1413-1421.
[14]	JIANG Hongzheng, YANG Dezhi, MA Zhonghua, XUN Wenjuan, SHI Liguang, HOU Guanyu. Genome-wide Association Study of Body Size Traits in Danzhou Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 598-607.
[15]	ZHOU Ya, ZHANG Heyang, LIU Linling, LI Haodong, ZHENG Junjun, WANG Guiwu. Polymorphism of MSRB3 Gene and Its Association Analysis with Antler Weight Traits in Sika Deer [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(11): 4279-4287.