黄酮和维生素E对己烯雌酚所致雄性小鼠肝脏氧化损伤的保护作用

Abstract

Abstract: The purpose of this study was to investigate the oxidative damage caused by different doses of diethylstilbestrol (DES) and the protective effects for DES induced toxicity with flavonoids and vitamin E in Kunming mice. 80 Kunming male mice were randomly divided into 10 groups, the groups were normal saline control group, DES exposed groups (0.035, 0.35, 3.5 mg/(kg穌)), flavonoids interference groups(26.0, 58.0, 112.0 mg/(kg穌)) and vitamin E interference groups(56.2, 112.4, 224.8 mg/(kg穌)). The effects on liver and testis were studied in adult male mice treated for one week. Mice were killed to collect liver and testis, and then contents of malonadehyde (MDA), superoxide dismutase (SOD), glutathione perxidase (GSH-Px) and total antioxidant capacity (T-AOC) were determined. It was found that high dose DES (3.5 mg/(kg穌)) could induce mice liver oxidative injury. The protective effects of flavonoids for DES induced toxicity on liver dose dependent increased. The contents of MDA significantly decreased (P<0.01), but the contents of SOD significantly increased in the high dose group (112 mg/(kg穌)). The contents of MDA significantly decreased (P<0.01), but the contents of GSH-Px, SOD and T-AOC significantly increased in the medium and high dose groups (112.4, 224.8 mg/(kg穌)). DES appeared to cause oxidative injury in Kunming mice. The results provided preliminary experimental support for flavonoids and vitamin E as medicine for DES induces damage.

Key words: diethylstilbestrol; flavonoids; vitamin E; oxidative injury

CLC Number:

Q579.1⁺3

DU Juan, PAN Hong-yan, LIN Li-mei, HUANG Da-wei, GONG Zhi-yong. Protection Effects of Flavonoids and Vitamin E on Diethylstilbestrol Induced Oxidative Injury[J]. , 2013, 40(4): 149-153.

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Protection Effects of Flavonoids and Vitamin E on Diethylstilbestrol Induced Oxidative Injury

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