辣蓼黄酮正丁醇部分体外抗猪繁殖与呼吸综合征病毒的研究

doi:10.16431/j.cnki.1671-7236.2020.02.033

Abstract

Abstract: To investigate the effect of n-butanol part of flavonoids from Polygonum hydropiper L.(FNB) against porcine reproductive and respiratory syndrome virus (PRRSV) in vitro,in this study,Marc-145 cells and PRRSV (TJM-F92) were used as the objectives,the toxicity of FNB on cells was detected by CCK-8 method.In addition,the inhibition rate of the FNB on PRRSV after the cells were treated by three methods (drug administration before PRRSV inoculation,drug administration post PRRSV inoculation,drug administration and PRRSV inoculation simultaneously).The results showed that the maximum safe concentration of FNB was 500 μg/mL.Therefore,FNB in the concentration range of 25-500 μg/mL was selected for subsequent experiments.Various concentrations of FNB could inhibit the proliferation of PRRSV on Marc-145 cells to varying degrees and exhibited a dose-dependent relation,which meant the higher the concentration of the drug,the better the antiviral effect.Among them,the last two treatment methods had significant anti-PRRSV effects,the cell viability was 21.55%-65.23% and 24.85%-73.60% respectively in the concentration range of 25-500 μg/mL.However,the infection of PRRSV after first administration couldn’t effectively reduce the infectivity of the PRRSV and the cell survival rate was only 7.00% at the highest dose of 500 μg/mL,so the antiviral effect was not obvious.Although the pretreatment of FNB on Marc-145 cells did not reduce the ability of PRRSV infection,the preventive effect of FNB on PRRSV cells was not satisfactory.However,the effect of FNB on the clinical treatment of PRRSV was considerable.FNB could inhibit the synthesis and release of PRRSV and directly kill of PRRSV,and then effectively inhibit the expression of PRRSV in Marc-145 cells.The results of this study not only provide a reference for the clinical treatment of PRRSV,but also offer theoretical basis for the further development and utilization of Polygonum hydropiper L..

Key words: n-butanol part of flavonoids from Polygonum hydropiper L.(FNB); PRRSV; antiviral; inhibitory effect; cell viability

CLC Number:

S859.7

ZHOU Shumian, CAO Mixia, HU Tingjun. A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(2): 605-611.

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A Study on Effect of Anti-PRRSV of n-butanol Part of Flavonoids from Polygonum hydropiper L. in vitro

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