China Animal Husbandry and Veterinary Medicine ›› 2021, Vol. 48 ›› Issue (5): 1558-1565.doi: 10.16431/j.cnki.1671-7236.2021.05.006

• Biotechnology • Previous Articles     Next Articles

Effect of Vitamin E on H2O2-Induced Oxidative Damage and Tight Junction Related Gene Expression of MAC-T Cells

ZHANG Ya1,2, BAI Ge1,2, WANG Tao1,2,3, QIN Guixin1,2, SUN Zhe2,3,4, ZHANG Xuefeng1,2,3, ZHEN Yuguo1,2,3   

  1. 1. Key Laboratory of Animal Production and Product Quality and Safety, Ministry of Education, Jilin Provincial Key Laboratory of Animal Nutrition and Feed Science, College of Veterinary Medicine, College of Animal Science and Technology, Jilin Agricultural University, Changchun 130118, China;
    2. JLAU-Borui Dairy Science and Technology R&D Center, Jilin Agricultural University, Changchun 130118, China;
    3. Feed Engineering Technology Research Center of Jilin Province, Changchun Borui Agriculture and Animal Husbandry Group Co., Ltd., Changchun 130114, China;
    4. College of Life Science, Jilin Agricultural University, Changchun 130118, China
  • Received:2020-10-19 Online:2021-05-20 Published:2021-05-20

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Abstract: This experiment aimed to study the effect of vitamin E (VE) on H2O2-induced oxidative damage and tight junction-related gene expression in MAC-T cells.There were three groups in this study,control,H2O2 and H2O2+VE groups.MTT was used to detect cell survival rate,while colorimetric method was employed to detect the activity of superoxide dismutase (SOD) and catalase (CAT),the content of malondialdehyde (MDA) and reactive oxygen species (ROS) in cell culture medium.The expression levels of tight junction genes ZO-1,occludin and claudin-1 were detected by Real-time PCR.The results showed that compared with the control group,the survival rate of MAC-T cells in the H2O2 group decreased significantly (P<0.05),the content of ROS and MDA increased significantly (P<0.05),and the activity of SOD and CAT decreased significantly (P<0.05).Compared with the H2O2 group,the cell survival rate of the H2O2＋20 μmol/L VE group increased significantly (P<0.05),the content of ROS and MDA decreased significantly (P<0.05),and the activity of SOD and CAT increased significantly (P<0.05).In addition,compared with the control group,the H2O2 group extremely significantly inhibited the relative expression of tight junction genes (P<0.01).Compared with the H2O2 group,the relative expression of tight junction genes was extremely significantly increased after the addition of vitamin E (P<0.01).This study demonstrated that vitamin E could lessen the oxidative damage induced by H2O2 in MAC-T cells,and significantly alleviate the down-regulate tight junction genes expression in MAC-T cells induced by H2O2.

Key words: vitamin E; dairy cow; oxidative stress; MAC-T cells; tight junctions

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