中国荷斯坦牛Toll样受体4基因的遗传多态性与体细胞评分的关联分析

Abstract

Abstract: The polymorphism of toll-like receptor 4(TLR4) gene and its correlation with somatic cell score (SCS) in Chinese Holstein cattle were analyzed for the purpose of providing molecular markers in the marker-assisted selections (MAS) to accelerate the disease-resistant breeding progress in Chinese Holstein cattle. The polymorphism of TLR4 gene was detected by PCR-RFLP and PCR-SSCP technique in 610 Chinese Holstein cattle cattle from 30 bull families. The associations between polymorphic sites of TLR4 gene and SCS were analyzed by the least square mean analysis. Two SNPs were found, SNP -226 G>C was found in the 5' flanking region of TLR4 gene, three genotypes (GG, GC and CC) were found in 610 Chinese Holstein cattle by PCR-RFLP, and the frequencies of GG, GC and CC genotypes were 0.208, 0.482 and 0.310, respectively. SNP 1760 C>T was found in the exon 3 of TLR4 gene, three genotypes (GG, GC and CC) were found in 610 Chinese Holstein cattle by PCR-SSCP, and the frequencies of CC, TC and TT genotypes was 0.738, 0.225 and 0.007, respectively. Chi-square tested showed Chinese Holstein cattle was not in Hardy-Weinberg equilibrium at the two sites. There were no significant differences in SCS among genotypes for SNP -226 G>C, least square mean of SCS for CC genotype was very significantly lower than that for TT and TC genotypes(P<0.01). CC genotype for SNP 1760 C>T site had a great genetic effect on SCS in Chinese Holstein cattle, which could be used as a candidate marker for mastitis resistance selection in dairy cattle.

Key words: Chinese Holstein cattle; TLR4 gene; PCR-RFLP; PCR-SSCP; polymorphism; SCS

CLC Number:

S813.3

CHEN Ren-jin, WANG Zhen-zhen, YANG Zhang-ping, ZHU Xiao-rong, JI De-jun, MAO Yong-jiang. Genetic Polymorphism of TLR4 Gene and its Associations with Somatic Cell Score in Chinese Holstein Cattle[J]. , 2013, 40(11): 134-138.

References

1 王兴平, 许尚忠, 马腾壑,等. 牛TLR4基因5'侧翼区的遗传变异与乳房炎的关联[J]. 遗传, 2006, 28(12): 1520~1524.
2 王兴平, 许尚忠, 马腾壑,等. 牛TLR4基因的遗传多态性与乳房炎的关联分析[J]. 畜牧兽医学报, 2007, 38(2): 120~124.
3 刘文娇, 孙少华, 李雪梅,等. TLR4基因外显子Ⅲ Bbv12Ⅰ酶切位点多态性与奶牛乳房炎的相关分析[J]. 中国农业科技导报, 2008, 10(2): 114~118.
4 袁芳,刘明军,白杰,等. 新疆褐牛与荷斯坦牛NF-κB基因表达与Toll样受体4基因型的关系[J].中国畜牧兽医, 2010, 37(10): 114~119.
5 Beutler B. TLR4: Central component of the sole mammalian LPS sensor. Curr Opin Immunol, 2000, 12(1): 20~26.
6 Beecher C, Daly M, Childs S,et al. Polymorphisms in bovine immune genes and their associations with somatic cell count and milk production in dairy cattle. BMC Genet, 2010, 11:99.
7 Goldammer T, Zerbe H, Molenaar A,et al. Mastitis increases mammary mRNA abundance of -defensin 5, toll like-receptor 2 (TLR2), and TLR4 but not TLR9 in cattle. Clin Diagn Lab Immunol, 2004, 11(1): 174~185.
8 Miller S I, Ernst R K, Bader M W. LPS, TLR4 and infectious disease diversity. Nat Rev Microbiol, 2005, 3:36~46.
9 Mucha R, Bhide M R, Chakurkar E B,et al. Toll-like receptors TLR1, TLR2 and TLR4 gene mutations and natural resistance to Mycobacterium avium subsp. paratuberculosis infection in cattle. Vet Immunol Immunopathol,2009,28:381~388.
10 Kataria R S, Tait R G Jr, Kumar D, et al. Association of toll-like receptor four single nucleotide polymorphisms with incidence of infectious bovine keratoconjunctivitis (IBK) in cattle. Immunogenetics, 2011, 63(2):115~119.
11 Ruiz-Larraaga O, Manzano C, Iriondo M,et al. Genetic variation of toll-like receptor genes and infection by Mycobacterium avium ssp. paratuberculosis in Holstein-Friesian cattle. J Dairy Sci, 2011, 94(7):3635~3641.
12 Sambrook J, Russell D W, Huang P T. Molecular cloning: A laboratory manual. Beijing: Science Press, 2002.
13 Sharma B S, Leyva I, Schenkel F, et al. Association of toll like receptor 4 polymorphisms with somatic cell score and lactation persistency in Holstein bulls. J Dairy Sci, 2006, 89(9): 3626~3635.
14 White S N, Taylor K H, Abbey C A, et al. Haplotype variation in bovine toll-like receptor 4 and computational prediction of a positively selected ligand-binding domain. Proc Natl Acad Sci USA, 2003, 100: 10364~10369.
15 Werling D, Hope J C, Howard C J, et al. Differential productionof cytokines, reactive oxygen and nitrogen by bovine macrophages and dendritic cells stimulated with toll-like receptor agonists. Immunology, 2004, 111(1): 41~52.

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Genetic Polymorphism of TLR4 Gene and its Associations with Somatic Cell Score in Chinese Holstein Cattle

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