Abstract: Polymorphism of the serum proteins for the pony in Guizhou province was carried out by native polyacrylamide gel electrophoresis (native-PAGE). Based on the band types of three proteins on native-PAGE,the protein variant types and its gene allele frequency were compared among albumin (Alb),α^1-B glycoprotein (AlB) and transferring (TF) proteins. Both of the Alb and TF loci,except for AlB,showed polymorphisms. Six protein variants were found from the Alb protein of Guizhou pony and Southwest horse,which corresponded to three alleles of A,B and C. Gene frequencies of Alb loci in Guizhou pony were A (0.2097),B (0.5645) and C (0.2258),respectively. Seven variant types were found in the TF protein from Guizhou pony,which was controlled by five gene alleles (A,B,C,D and E). Gene frequencies of TF in Guizhou pony were A (0.0484),B (0.5806),C (0.1129),D (0.1452) and E (0.1129),respectively. The polymorphism information content (PIC) and heterozygosis (H) were higher at Alb and TF loci compared with other Chinese ponies and horses. Furthermore,the power of discrimination (DP) and probability of exclusion (PE) in Guizhou pony were higher than others. It suggested that the genetic diversity of Guizhou pony was higher and both of Alb and TF protein polymorphism might be used to identify parentage and individuals of Guizhou pony from each other, and lower heterozygosis of Guizhou pony implied a higher gene purity of Guizhou pony.

Key words: Guizhou pony; serum proteins; polymorphism