内化菌耐受性Tr值评估方法研究

doi:10.16431/j.cnki.1671-7236.2024.05.045

Abstract

Abstract: 【Objective】 This study was aimed to develope a comprehensive evaluation method that considered the interaction of host cells,internalized bacteria and antimicrobial agents to accurately compare internalized bacteria-mediated antimicrobial resistance.【Method】 Using IEC-6,RIMVECs and RAW264.7 cells as host cells,Staphylococcus aureus and Salmonella as internalized bacteria,seven chemical drugs including ampicillin,ciprofloxacin,tetracycline,and four traditional Chinese medicine standards including quercetin and kaempferol as antibacterial drugs,the minimum extracellular bactericidal concentration (MBC_EX) of antibiotics was detected,and the bacteria-cell interaction model was established.The minimum intracellular bactericidal concentration (MBC_IN) of antibiotics was detected by bacterial colony count.The percentage of endobacteria-infected cells (N) and the percentage of endobacteria-infected cells (N_D) after drug treatment were measured.The drug tolerance value (Tr) was calculated to evaluate the antimicrobial resistance mediated by internalized bacteria.【Result】 Staphylococcus aureus was resistant to ampicillin,ciprofloxacin,erythromycin,polymyxin,rifampin,tetracycline,vancomycin and baicalin (Tr＞1).Among them,the Tr value of ciprofloxacin was the highest and the tolerance was the most serious.It did not produce tolerance to quercetin,kaempferol and catechin (Tr≤1).The results of tolerance of different internalized bacteria to tetracycline showed that the Tr value of resistance of Staphylococcus aureus to tetracycline was greater than that of Salmonella.The influence of different host cells on internalized bacteria-mediated antimicrobial resistance showed that Staphylococcus aureus had the highest tolerance to tetracycline in IEC-6 cells.【Conclusion】 The Tr value evaluation method of tolerance could not only effectively evaluate the tolerance degree of internalized bacteria to different antibiotics,but also compare the influence of different host cells on the resistance to antibiotics mediated by internalized bacteria,and visually and data-present the tolerance of internalized bacteria to antibiotics,providing guidance for clinical precision medicine.

Key words: internalized bacteria; antibacterial drugs; tolerance; assessment of tolerance value

CLC Number:

SUN Zhigang, WANG Xiao, SI Xiaohui, LYU Ruoyi, SONG Lianyu, LIU Xiaoye. Study on Assessment of Internalized Bacteria-mediated Antimicrobial Tolerance Tr Value[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(5): 2245-2252.

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Study on Assessment of Internalized Bacteria-mediated Antimicrobial Tolerance Tr Value

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