广西某地区猪源大肠杆菌耐药性及部分菌株耐药基因调查

doi:10.16431/j.cnki.1671-7236.2019.12.035

Abstract

Abstract: The aim of this study was to analyze the drug resistance of porcine Escherichia coli in some areas of Guangxi and drug resistance genes contained in some strains,and provide a reference for the prevention and treatment of animal Escherichia coli diseases.The experiment used Macconkey agar medium and Eosin-Methylene Blue agar medium preliminary identification,microscopic microscopy,molecular biology 16S rRNA identification,drug sensitivity double broth microdilution method and other methods to isolate and identify Escherichia coli,combined with PCR-related techniques were used to detect more than 30 drug resistance genes in some strains.The results showed that 65 strains of porcine Escherichia coli were isolated from pig-derived materials collected from a certain area of Guangxi,all of which were multi-drug resistant Escherichia coli and concentrated in six-drug resistance and seven-drug resistance.According to the investigation of the antibiotic-resistant drugs,it was found to be highly resistant to β-lactams,sulfonamides,macrolides,and the drug resistance rates were 98.46%,90.77% and 84.62%,respectively.Further analysis results found that it was mainly resistant to antibiotics such as ceftiofur sodium,amoxicillin,sulfamonomethoxine,azithromycin,and sensitive to meropenem,amikacin and colistin.The resistance genes contained in some strains were found to contain tetracycline (tetA and tetM),β-lactams (CTX-m-u) and sulfonamide (Sul2) resistance genes.In summary,it was found that the drug resistance of porcine Escherichia coli in Guangxi was serious,and there were relatively many drug resistance genes.Therefore,in the actual production,it was necessary to use related measures such as combination therapy for disease treatment to achieve rapid and effective treatment of diseases.

Key words: porcine Escherichia coli; antibacterial drugs; drug resistance; drug resistance gene

CLC Number:

S852.61⁺2

LI Chong, CHEN Yunru, LI Qinmei, SUN Junying, AN Zhaoxia, QIN Fenglin, CHEN Xinyu, SHEN Shuibao, SI Hongbin. Analysis of Drug Resistance of Porcine Escherichia coli and Drug Resistance Genes of Some Strains in a Region of Guangxi[J]. China Animal Husbandry and Veterinary Medicine, 2019, 46(12): 3749-3758.

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Analysis of Drug Resistance of Porcine Escherichia coli and Drug Resistance Genes of Some Strains in a Region of Guangxi

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