广东省某屠宰场猪源肠球菌的分离鉴定及耐药性和毒力基因分析

doi:10.16431/j.cnki.1671-7236.2023.11.038

Abstract

Abstract: 【Objective】 The purpose of this experiment was to understand the antibiotic resistance present status and virulence factor carrier of swine origin Enterococcus, provide data support for the monitoring of Enterococcus antibiotic resistance status and provide scientific basis for formulating reasonable treatment plan.【Method】 In this study, 156 swine small intestine samples from a large slaughterhouse in Guangzhou were isolated, cultured, Gram stained, and identified by PCR.The minimum inhibitory concentration of 14 kinds of antibiotics and 2 kinds of disinfectants were determined by agar diffusion method, and the carrying of drug resistance genes, virulence genes, and antiseptic resistance genes were detected by PCR technology.【Result】 The results showed that 84 strains of Enterococcus were isolated in this research (the separation rate was 53.85%), including 44 E.faecalis and 40 E.faecium.Isolation and culture showed that the colony edges were smooth and neat, round or oval arranged, and the medium around the colony showed black colonies.Suspected colonies of Gram positive cocci that appear circular after Gram staining, arranged individually or in piles.The DNA of the suspected colony was amplified by PCR.The bands of about 941 bp were amplified by E.faecalis primer, and the bands of about 1 500 bp were amplified by 16S rRNA primer.Drug sensitivity test results showed that 44 strains of E.faecalis were highly resistant to clindamycin, doxycycline, ceftiofur, gentamicin, erythromycin, benzalkonium chloride and flufenicol, and antibiotic resistance rates were 97.7%, 90.9%, 88.6%, 88.6%, 81.8%, 81.8% and 77.3%, respectively.40 strains of E.faecium were resistant to clindamycin, ceftiofur, doxycycline, gentamicin, flufenicol and erythromycin, and the resistance rates were 90.0%, 90.0%, 85.0%, 77.5%, 72.5% and 67.5%, respectively.The frequency of efa, gelE and fsrC genes were found to be high in the E.faecalis virulence genes, and the detection rates were 95.5%, 84.1% and 84.1%, respectively.The detection antiseptic resistance gene rates of emeA and gsp65 genes were 95.5% and 90.9%, respectively.Only fsrB virulence gene was detected in E.faecium, the detection rate was 2.5%, while the isolates were negative for any antiseptic resistance genes.【Conclusion】 The isolated Enterococcus showed high resistant to most antibacterial drugs, and a part of Enterococcus were resistant to linezolid, so swine-derived Enterococcus epidemiology needed to be monitored.

Key words: Enterococcus; drug resistance; virulence genes; antibacterial drugs; antiseptic resistance gene

CLC Number:

S852.61^＋1

ZHANG Yongxiang, HU Jianxin, LU Yixing, HU Yueying, LU Jiaxuan, XIN Lin, ZENG Zhenling, ZENG Dongping. Isolation and Identification of Swine-derived Enterococcus and Analysis of Drug Resistance and Virulence Genes from a Slaughterhouse in Guangdong Province[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(11): 4714-4723.

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Isolation and Identification of Swine-derived Enterococcus and Analysis of Drug Resistance and Virulence Genes from a Slaughterhouse in Guangdong Province

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