›› 2018, Vol. 45 ›› Issue (4): 898-904.doi: 10.16431/j.cnki.1671-7236.2018.04.008

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Biological Characteristics of Eleven Bacillus Strains from Rumen

TAN Shiyao, ZHANG Xue, WANG Hongying, QIANSIRI Guleng   

  1. College of Biological Engineering, Dalian Polytechnic Univerity, Dalian 116034, China
  • Received:2017-09-21 Online:2018-04-20 Published:2018-04-25

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Abstract:

The biological characteristics of eleven Bacillus strains from rumen were studied,including the activity of produced enzyme,the endurance to acid and bile salt,the activity of antibacteria and the compatibility.3,5-dinitrosalicylic acid colorimetric method (DNS method) was used to determine the enzyme activity,acid resisting test and bile salt resisting test were conducted by plate spreading method,antibacterial activity was determiend by Oxford cup method,and while indoor compatibility experiment was studied by filter paper method.The results showed that the activity of cellulase produced by T-2,T-3 and T-10 were higher;T-2,T-5,T-7,T-8 and T-9 had stronger acid resistance;T-4,T-5,T-9 and T-11 had stronger ability of bile salt-tolerance;T-4 and T-7 had stronger antibacterial activity against Vibrio parahaemolyticus,and T-7 had strongest antibacterial activity against Aerogenes,T-5,T-9 and T-10 had stronger antibacterial activity against Escherichia coli and T-3 had strongest antibacterial activity against Sarcina lutea.Indoor compatibility experiments showed that there were antagonistic effects among some strains of eleven Bacillus strains.The combinations of the selected strains for bacteriostat were T-3,T-4,T-5;T-3,T-4,T-10;T-4,T-5,T-10;T-3,T-7,T-10.The combinations of the selected strains for higher cellulase activities were T-2,T-3,T-5;T-2,T-3,T-10;T-2,T-5,T-10;T-3,T-5,T-6;T-3,T-5,T-10.The results could provide some references for the research and development of animal feed.

Key words: Bacillus; biological characteristics; tolerance; compatibility

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