梅花鹿瘤胃纤维素降解菌的分离鉴定及其酶活力测定

doi:10.16431/j.cnki.1671-7236.2018.04.007

Abstract

Abstract:

In order to obtain strains with higher cellulose-degrading ability isolated from Sika deer (Cervus nippon) rumen,the fresh rumen liquid was collected from Sika deer,and CMC-Na medium was used to isolate and screen strains,and the morphological,physiological and biochemical characteristics and 16S rDNA gene sequence were analyzed to identify the taxonomic position of the strains.Moreover,the cellulose producing conditions of the strain was preliminarily studied by DNS method,and the characteristics of cellulose production with different substances as substrates were studied in order to provide theoretical basis for the application of strain.The results showed that N-11 strain was a highly efficient cellulose-degrading bacteria,which was identified as Bacillus cereus.The properties of the cellulose production showed that when the fermentation temperature ranged from 30 to 45℃ (optimum at 40℃),pH was 6.0 to 7.0 (optimum at 6.0),and carbon source content was 2% to 5% (optimum at 5%),it had the higher enzyme activity.In addition,when cultivated for 36 h,the peak of cellulose production was reach which CMC cellulose activity of N-11 strain was 12.563 U/mL and FPA cellulose activity was 12.414 U/mL.The relative cellulose activity still retained above 80% at 20 to 50℃ or pH 6.0 to 8.0 when cultivated for 1 h.These results indicated that N-11 strain had a high cellulose productivity,and it might have some potential for industrial applications.

Key words: Sika deer rumen; cellulose; isolation and identification; enzyme activity; Bacillus cereus

CLC Number:

S816.73

WEI Haiting, LIU Hanlu, LI Guangyu, SI Huazhe, LIU Xiaoying. Isolation, Identification and Determinations of Cellulose-degrading Bacteria from Sika Deer (Cervus nippon) Rumen[J]. , 2018, 45(4): 888-897.

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Isolation, Identification and Determinations of Cellulose-degrading Bacteria from Sika Deer (Cervus nippon) Rumen

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