白藜芦醇对山羊冷冻精子质膜、DNA完整性和温度耐受性的影响

doi:10.16431/j.cnki.1671-7236.2020.01.015

Abstract

Abstract: The aim of this study was to investigate the effects of resveratrol on plasma membrane,DNA integrity and thermal tolerance of frozen goat sperm.Semen was collected from 8 Yunshang Black goats using artificial vagina.Semen was diluted with the Optidyl extender supplemented with resveratrol at 0,0.1,1,10 and 20 μmol/L.Then the diluted semen was floaded into IMV plastic straw.The semen frozen without resveratrol was used as control group.After equilibration at 5 ℃ for 4 h,the straws were pre-frozen in liquid nitrogen vapor for 10 min,followed by storage in liquid nitrogen for 30 d.After thawing in 37 ℃ water bath,the plasma membrane integrity and temperature tolerance were measured by low osmolality tolerance test and sperm chromatin diffusion method was used to detect DNA fragmentation rate.The results showed that the rate of sperm with curled tail in 10 μmol/L resveratrol freezing group was significantly higher than that in other groups (P<0.05),but there was no significant difference in other groups (P>0.05).The DNA fragmentation rate of 10 μmol/L resveratrol freezing group was extremely significantly higher than that of fresh sperm (P<0.01),and was extremely significantly lower than that in non-resveratrol control group (P<0.01).The results of thermal tolerance test showed that the sperm tail-bending rate was the highest when sperm frozen with 10 μmol/L resveratrol were equilibrated in 37 ℃ water bath for 1 h,and there was extremely significant difference between 10 μmol/L resveratrol freezing group and the other freezing groups (P<0.01).The rate of sperm with curled tail decreased gradually with prolonging of the incubation time.After incubation for 4 h,there was no significant difference in the rate of sperm with curled tail between 10 μmol/L resveratrol freezing group and control group (P>0.05).The rate of sperm with intact plasma membrane in 10 μmol/L resveratrol freezing group was significantly higher than that in control group (P<0.05).In conclusion,resveratrol could improve the plasma membrane status,DNA integrity and thermal tolerance of frozen goat semen.The optimally functional concentration of resveratrol was 10 μmol/L.However,whether resveratrol could improve the effect of artificial insemination of frozen goat semen remains to be studied.

Key words: goat semen; resveratrol; freeze; plasma membrane; DNA; thermal tolerance

CLC Number:

S814.8

LYU Chunrong, Allai Larbi, LI Junxi, HONG Qionghua, QUAN Guobo. Effects of Resveratrol on Plasma Membrane,DNA Integrity, and Thermal Tolerance of Frozen Goat Sperm[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 124-130.

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Effects of Resveratrol on Plasma Membrane,DNA Integrity, and Thermal Tolerance of Frozen Goat Sperm

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