猪DNA甲基转移酶基因家族进化分析

doi:10.16431/j.cnki.1671-7236.2022.09.002

Abstract

Abstract: 【Objective】 The purpose of this study was to investigate the gene characteristics and expression patterns of DNA methyltransferase (DNMT) gene family in pig.【Method】 Bioinformatics methods were used to identify members of porcine DNMT gene family at the whole genome level, and the chromosomal localization, physicochemical properties, protein structure, subcellular localization, gene structure, conserved motif, phylogenetic relationship, collinear relationship and expression pattern of DNMT gene were analyzed.【Result】 A total of five porcine DNMT genes:PigDNMT1, PigDNMT2, PigDNMT3, PigDNMT4 and PigDNMT5, were identified, which were distributed on chromosomes 2, 3, 13, 14 and 17, respectively.Phylogenetic tree analysis of porcine DNMT gene family found that PigDNMT1 and PigDNMT4, as well as PigDNMT2 and PigDNMT5 displayed closer relationships.PigDNMT2 and PigDNMT5 were identical in number and order of motifs, but differ in gene structure.Phylogenetic tree analysis revealed that the DNMT gene family of pig, human, mouse and cattle were divided into four subfamilies, among which the DNMT gene family of pig and cattle displayed closer relationships.Gene collinearity analysis showed that there were four pairs of orthologous genes for DNMT in pig, human, mouse and cattle, indicating that there was a strong collinear relationship among the DNMT genes of the four species.Lengths of DNMT family protein in pig varied from 228 to 1 706 amino acids, and the molecular weight of DNMT proteins varied from 26 133.32 to 192 267.80 u, and the isoelectric points of DNMT protein varied from 6.00 to 9.06.Analysis of secondary structure prediction showed that DNMT proteins of pig were mainly composed of random coils.Subcellular localization analysis found that all DNMT genes of pig were located in the nucleus.Transcriptome analysis of sow gonadal axis found that PigDNMT1 showed different expression patterns in the hypothalamus, pituitary and ovary during the onset of puberty.【Conclusion】 In this study, a total of 5 DNMT genes were identified in pig, and it was found that PigDNMT1 in the gonadal axis showed different expression patterns during the initiation of puberty.This bioinformatics analysis provided some reference for analyzing the function of DNMT gene family in pigs.

Key words: pig; DNA methyltransferase (DNMT); phyletic evolution; expression analysis

CLC Number:

S828

GONG Wentao, PAN Xiangchun, CAI Jiali, WANG Yifei, LI Jiaqi, ZHANG Zhe, YUAN Xiaolong. Evolutionary Analysis of DNA Methyltransferase Gene Family in Pig[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3283-3291.

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Evolutionary Analysis of DNA Methyltransferase Gene Family in Pig

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