延黄牛ALDH1A1基因内含子4多态性及其与肉用性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2018.11.021

Abstract

Abstract:

This study was aimed to explore the effect of the polymorphism of acetaldehyde dehydrogenase 1A1 (ALDH1A1) gene intron 4 on meat traits in Yanhuang cattle.99 Yanhuang cattle at 18 months old were selected,the single nucleotide polymorphism (SNP) of ALDH1A1 gene intron 4 in Yanhuang cattle were detected by direct sequencing of PCR amplification products,and the correlation between ALDH1A1 gene intron 4 SNP and meat traits (body height,body length,cross height,chest circumference,abdominal circumference,tube circumference,body weight,back fat thickness,loin eye area and intramuscular fat) were analyzed using SPSS 19.0 software.The results showed that a C/T mutation had been found in ALDH1A1 gene intron 4,there were three genotypes (TT,TC and CC) and two alleles (T and C),TC was the dominant genotype,and C was the dominant allele.The chi-square test result showed that Yanhuang cattle population was in Hardy-Weinberg equilibrium (P>0.05).Population genetic parameter analysis showed the heterozygosity (He) of the mutation site was relatively low,there was a low varitation in Yanhuang cattle population,it was a moderately polymorphic (0.25<PIC<0.5),and the genetic marker could provide genetic information.There was no significant difference between the different genotypes and meat traits in Yanhuang cattle (P>0.05).The results indicated that the polymorphism of ALDH1A1 gene intron 4 was found in Yanhuang cattle,it needed to expand the number of sample for further explore whether the site could be considered as genetic markers for meat traits in Yanhuang cattle or not.

Key words: ALDH1A1 gene; Yanhuang cattle; intron 4; polymorphism; meat traits

CLC Number:

S813.3

HU Zhongchang, CAO Yang, WU Jian, QIN Lihong, JIN Haiguo, ZHAO Yumin. Polymorphism of ALDH1A1 Gene Intron 4 and Its Association with Meat Traits in Yanhuang cattle[J]. , 2018, 45(11): 3153-3159.

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Polymorphism of ALDH1A1 Gene Intron 4 and Its Association with Meat Traits in Yanhuang cattle

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