›› 2018, Vol. 45 ›› Issue (6): 1580-1589.doi: 10.16431/j.cnki.1671-7236.2018.06.020

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Expression Patterns of PLIN2 Gene in Subcutaneous Adipose Tissue and Association Analysis of Single Nucleotide Polymorphisms with Meat Quality Traits in Qinchuan Cattle

LI Peiwei1, WU Sen1, WANG Yaning1, WANG Hongbao1,2, ZAN Linsen1,2   

  1. 1. College of Animal Science and Technology, Northwest A & F University, Yangling 712100, China;
    2. National Beef Cattle Improvement Center, Yangling 712100, China
  • Received:2017-10-17 Online:2018-06-20 Published:2018-06-15

Abstract:

This study investigated the expression of PLIN2 gene in subcutaneous adipose tissue and analyzed the association between PLIN2 gene single nucleotide polymorphism (SNPs) and meat quality traits in Qinchuan cattle.The mRNA expression levels of PLIN2 gene in subcutaneous adipose tissue from 6 to 60 months old Qinchuan cattle was detected by Real-time quantitative PCR.536 healthy Qinchuan cattles aged from 18 to 24 months old were selected to collect blood samples and determine the meat quality traits.The expression of PLIN2 gene in subcutaneous adipose tissue increased from 6 months old and peaked at 18 months old with the decreasing till 60 months old.The results showed that 9 SNPs of PLIN2 gene were identified by PCR amplification and direct sequencing,the intramuscular fat content (IMF) of CT genotype in g.C7919>T site was significantly higher than that of TT and CC genotypes (P<0.05).The back fat thickness (BFT) of CC and CT genotypes in g.C7933>T site were significantly higher than that of TT genotype (P<0.05),the IMF of CT genotype was significantly higher than that of TT and CC genotypes (P<0.05).The IMF of CC genotype in g.G8015>C site was significantly higher than that of GG and GC genotypes (P<0.05).The IMF of TC genotype in g.T8496>C site was significantly higher than that of CC and TT genotypes (P<0.05),the BFT of TT genotype in g.C8578>T site was significantly higher than that of CC and CT genotypes (P<0.05).PLIN2 gene could be considered as a candidate reference for Qinchuan cattle meat traits researching,and provided a certain basis for further breeding.

Key words: Qinchuan cattle; PLIN2 gene; expression pattern; polymorphism; association analysis

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