绵羊抗菌肽GNLY基因多态性分析及合成多肽的生物学功能研究

doi:10.16431/j.cnki.1671-7236.2018.05.025

Abstract

Abstract:

This study was aimed to analyze the polymorphism of granulysin (GNLY) gene of sheep antimicrobial peptide,detect the antibacterial activity and inhibition tumor cell activity of synthetic peptides,and clarify the biological function of sheep antimicrobial peptide GNLY.In this study,the polymorphism of GNLY gene was analyzed by sequencing RT-PCR products of GNLY gene,and its deduced amino acid fragments were synthesized,the antibacterial effect of synthetic peptides on Escherichia coli,Salmonella,Staphylococcus and Staphylococcus aureus were determined using radial diffusion test and MIC test,and its inhibition growth to human esophageal cancer cells (EC109) and human renal carcinoma cells (X786-0) were detected using MTT test.The results showed that the concentration of G16>250 μg/mL in the MIC test inhibited the growth of Escherichia coli and Staphylococcus aureus;The concentration of GS16 ≥ 7.82 μg/mL obviously inhibited the growth of Escherichia coli,there was no inhibitory effect on Salmonella,Staphylococcus and Staphylococcus aureus.62.5 μg/mL GC16 had inhibitory effect on all four strains (except for Staphylococcus) and obviously inhibited the growth of Escherichia coli and Staphylococcus aureus.G22 had no inhibitory effect on all four strains.G22 and GC16 obviously inhibited the growth of EC109 and X786-0,the growth inhibition rates against EC109 of 1 mg/mL G22 and 1 mg/mL GC16 were 88.21% and 57.21%,and its against X786-0 were 96.37% and 81.87%,respectively.G16 and GS16 had no inhibitory activity to tumor cells.This study indicated that the synthetic peptides of GNLY had inhibitory activity on bacteria and tumor cells,also laid the foundation for the development of sheep antibacterial peptides as candidate antibacterial medicine.

Key words: sheep antimicrobial peptide; GNLY gene; polymorphism; antibacterial test; MTT test

CLC Number:

TQ465.6

SONG Jiawei, YANG Xia, XIAO Shengzhong, LI Yan, CAI Zhuoxuan, ZHOU Ziheng, SHENG Jinliang, YAN Genqiang. Polymorphism Analysis of GNLY Gene of Sheep Antibacterial Peptide and Study on the Function of Synthetic Polypeptide[J]. , 2018, 45(5): 1319-1330.

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Polymorphism Analysis of GNLY Gene of Sheep Antibacterial Peptide and Study on the Function of Synthetic Polypeptide

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