延边黄牛ACTA1基因的SNPs检测及其与肉质性状的关联分析

doi:10.16431/j.cnki.1671-7236.2017.04.024

Abstract

Abstract:

This study was aimed to research the gentic variations of ACTA1 gene in Yanbian Yellow cattle, and analyze the influence of genetic polymorphisms on meat quality traits. PCR-RFLP and PCR-SSCP technique were used to analyze the association between the polymorphisms of ACTA1 gene and meat quality traits. The results showed that there were two SNPs, A193G which with replacement of Arg by Gly amino acid, A298G which with replacement of Lys by Arg amino acid. χ² test showed A193G and A298G were in Hardy-Weinberg equilibrium (P>0.05). The association analysis indicated that the genotypes of different mutations in ACTA1 gene were found to be significantly related to myristic acid, oleic acid, loin muscle area, marbling score (P<0.05). ACTA1 gene had potential effects on meat quality traits of Yanbian Yellow cattle, and could be used for candidate gene in early selection of new varieties of Yanbian Yellow cattle.

Key words: Yanbian Yellow cattle; ACTA1 gene; polymorphisms; meat quality trait

CLC Number:

S813.3

TIAN Wan-nian, YANG Han-lin, XIA Guang-jun, YAN Chang-guo. Polymorphism of ACTA1 Gene and Its Association with Meat Quality Traits in Yanbian Yellow Cattle[J]. , 2017, 44(4): 1108-1114.

References

[1] 贺淹才. 肌动蛋白和肌动蛋白基因的研究进展[J]. 生命的化学,2002,22(3):248-250.
[2] Craig R,Padron R. Molecular Structure of the Sarcomere[M]. New York:McGrawHill Prafessional,2004.
[3] 胡倩,曹允考,佟慧丽,等. 牛骨骼肌基因α-actin基因5'上游调控元件及内含子功能的初步分析[J]. 中国兽医学报,2015,35(7):1136-1142.
[4] Lee S H,Park E W,Cho Y M,et al. Identification of differentially expressed genes related to intramuscular fat development in the early and late fattening stages of Hanwoo Steers[J]. J Biochem Mol Biol,2007,40(5):757-764.
[5] Shin S C,Chung E R. Identification of differentially expressed genes between high and low marbling score grades of the longissimus lumborum muscle in Hanwoo (Korean cattle)[J]. Meat Sci,2016,121(5):114-118.
[6] 田万年,张守发,李香子,等. 延边黄牛背最长肌差异表达基因的筛选、克隆及序列分析[J]. 遗传,2011,33(11):1219-1224.
[7] Atencia R,Asumendi A,Garcia-Sanz M. Role of cytoskeleton in apoptosis[J]. Vitam Horm,2000,58:267-297.
[8] Luo L. Actin cytoskeleton regulation in neuronal morphogenesis and structural plasticity[J]. Annu Rev Cell Dev Biol,2002,18:601-635.
[9] Clarke N F,Ilkovskil B,North K,et al. The pathogenesis of ACTA1 related congenital fiber type disproportion[J]. Ann Neurol,2007,61(6):552-561.
[10] Feng J J,Marston S. Genotype phenotype correlations in ACTA1 mutations that cause eongenital myopathies[J].Neuromuscul Disord,2009,19(1):6-16.
[11] Juretic N,Urzua U,Riveros N,et al. Differential gene expression in skeletal muscle cells after membrane depolarization[J].J Cell Physiol,2007,5:478-481.
[12] Laing N G,Dye D E,Wallgren-Pettersson C,et al. Mutations and polymorphisms of the skeletal muscle alpha-actin gene (ACTA1)[J].Hum Mutat,2009,30(9):1267-1277.
[13] Huang J S,Xiong Y Z,Deng C Y. Cloning and expression analysis of porcine ACTA2 gene and its association with production traits[J].Yi Chuan,2009,31(5):489-494.
[14] Sewry C A,Holton J L,Dick D J,et al. Zebra body myopathy is caused by a mutation in the skeletal muscle actin gene (ACTA1)[J]. Neuromuscul Disord,2015,25(5):388-391.
[15] Venturini G C,Stafuzza N B,Cardoso D F,et al. Association between ACTA1 candidate gene and performance,organs and carcass traits in broilers[J]. Poult Sci,2015,94(12):2863-2869.
[16] 张斌,王静,宋敏艳,等.槟榔水牛ACTA1基因多态性与生长性状的相关性研究[J]. 中国牛业科学,2014,40(6):31-33.
[17] 侯丽,柴沙驼,刘书杰,等. 青海牦牛肉与秦川牛肉氨基酸和脂肪酸的比较研究[J]. 肉类研究,2013,27(3):30-36.
[18] 陈银基,周光宏,邹晓葵,等. 肉牛背最长肌脂肪酸组成与大理石花纹等级相关性研究[J]. 中国农业科学,2007,40(12):2853-2860.
[19] 郭盼盼,毛元廷,李香子,等. 延边黄牛公牛与阉牛牛肉营养成分比较研究[J]. 中国畜牧兽医,2016,43(9):2333-2338.
[20] 李静,曹阳,张立春,等. 延边黄牛PRKAG3基因第10外显子单核苷酸多态性及其与肉质性状的相关分析[J]. 中国畜牧兽医,2010,37(3):123-126.
[21] 龙小娟,房希碧,姜平,等. 中国西门达尔牛AGPAT6基因外显子多态性及其与肌内脂肪酸含量的相关性[J]. 中国兽医学报,2016,36(3):527-530.

Polymorphism of ACTA1 Gene and Its Association with Meat Quality Traits in Yanbian Yellow Cattle

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[2]	LI Mingbo, GAO Qingshan, LI Guanhao, ZHANG Kui, CUI Lianhua, HOU Lina. Effects of Chinese Herbal Medicine Feed Additives on Meat Quality and Flavor Substances in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(2): 531-542.
[3]	YANG Man, LIU Hai, ZHANG Run, HU Ziping, NIU Naiqi, WANG Lixian, ZHANG Longchao. Association Analysis of Polymorphism of MYH3 and MYH13 Genes with Meat Quality Traits in Beijing Black Pigs [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3428-3437.
[4]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[5]	FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.
[6]	CHEN Xuan, LYU Yanqiu, HAN Yue, XU Yichao, JIN Yi. Effect of Trehalose Replacing Partial Glycerol on Frozen-thawed Sperm Quality of Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(1): 256-264.
[7]	GUO Panpan, JIN Xin, SUN Jianfu, LI Qiang, LI Xiangzi, YAN Changguo. Cloning,Bioinformatics and Tissue Expression Analysis of DGATs Genes in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(9): 3158-3170.
[8]	CAO Xue, HUANG Xiaocheng, WANG Xuanli, TIAN Yihao, LI Chengyun. Effects of Adding Low-level Condensed Tannins to Different Nitrogen Sources on Rumen Fermentation Characteristics and Degradation Rate of Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(8): 2809-2819.
[9]	GUO Panpan, JIN Xin, SUN Jianfu, LI Xiangzi, YIN Yunhou, YAN Changguo. Analysis of Differentially Expressed Genes in Differentiation of Yanbian Yellow Cattle Preadipocytes Induced by Cycloglitazone and Insulin Based on RNA-Seq Data [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2358-2368.
[10]	LI Yunxia, YAO Xinkui, MENG Jun, ZENG Yaqi, WANG Jianwen, ZHENG Guopeng, ZHANG Yue, YUAN Xinxin, WANG Tongliang, LI Yuntao. Correlation Analysis Between Polymorphism of MyoG Gene Exon 1 With Meat Quality Traits and Muscle Fiber Traits in Yili Horse [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2504-2511.
[11]	ZHANG Jiasu, GUO Siwei, ZHANG Xinxin, ZHANG Congcong, ZHANG Shuai, YIN Baozhen, ZHANG Luomeng, XIA Guangjun. Genetic Polymorphism of LPL gene and Its Correlation Analysis of Meat Quality Traits in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(7): 2530-2538.
[12]	TIAN Quan, WANG Sihan, LIU Lei, ZHAO Yi, LIU Haixing, JIN Huazi, LI Zhongshu, ZHANG Junzheng, JI Shuang. Analysis of MYH3 and MYH8 Genes Polymorphisms and Their Correlation with Growth Traits in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(6): 2122-2128.
[13]	LIU Tian, GAO Qingshan, LI Xiangzi, YAN Changguo. Effects of Castration at Different Months on Fatty Acid Content in Muscle of High-grade Parts of Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4460-4467.
[14]	TIAN Quan, ZHAO Yi, WANG Zhuo, LIU Lei, ZHANG Junzheng, JI Shuang. Polymorphisms of lncRNA MEG3 Gene and Its Correlation with Growth Traits in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(12): 4567-4574.
[15]	ZHAO Yi, WANG Sihan, LIU Lei, TIAN Quan, LIU Haixing, LI Zhongshu, ZHANG Junzheng, JI Shuang. Polymorphism of UCP2 and UCP3 Genes and Its Association with Growth Traits in Yanbian Yellow Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2021, 48(10): 3691-3698.