6个猪品种CD53基因中结构变异SV184分布频率的研究

doi:10.16431/j.cnki.1671-7236.2017.04.020

Abstract

Abstract:

A candidate structure variation (SV) 184 was screened out from the resequencing data of Xiang pig. To explore the polymorphism of SV184 among pig breeds, the genotype distributions of SV184 in leukocyte antigen protein (CD53) gene was detected by PCR method taking six Chinese and European pig breeds as samples including Xiang pig,Large White pig,Nuogu pig,Kele pig,Jiangkouluobo pig and Rongchang pig breeds. Furthermore, the primary transcripts of three genotypes of SV184 were determined using RT-PCR technology. Results showed that the type VN was dominant in Xiang pig,while type NN was mainly in others. Both of two primary transcripts of CD53 gene were determined from the heterozygous genotype of SV184,which indicated that the presence of SV184 might not disturb the template selection in the process of CD53 gene transcription. It suggested that SV184 could be taken as one of molecular marker to distinguish Xiang pig from other pig breeds.

Key words: pig breeds; CD53 gene; structure variation; polymorphism; precursors

CLC Number:

S828.8

XU Qian, CEN Yong-xiu, YU Chang-yan, LIU Chang, RAN Xue-qin, WANG Jia-fu. Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds[J]. , 2017, 44(4): 1079-1086.

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Study on Distribution Frequency of Structure Variation SV184 in CD53 Gene of Six Pig Breeds

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