拜城油鸡肌肉生长抑制素基因多态性与生产性能的相关性研究

doi:10.16431/j.cnki.1671-7236.2017.04.017

Abstract

Abstract:

In this study, the single nucleotide polymorphism(SNP) of MSTN gene and its correlation with the production performance was tested, and explored the molecular genetic marker of breeding in Baicheng fatty chicken. 96 Baicheng fatty chicken roosters were selected randomly and divided into 3 groups, there were 32 chicken in each group. The indexes of production performance were measured in 12, 18 and 24 weeks old and the SNP of MSTN gene was detected by PCR-RFLP method, and analyzed the relationship between gene polymorphism and production performance indexes.The results showed that 3 genotypes of AA,GA and GG were found in Baicheng fatty chicken, GA was the dominant genotype, and allele A was the dominant allele, the allele frequency was not in the Hardy-Weinberg equilibrium (P<0.05). At 12 weeks old,AA genotype was significantly higher than GA genotype in body weight, carcass weight,semi-eviscerated weight, eviscerated weight, breast muscle weight and leg muscle weight (P<0.05). At 18 weeks old,GA genotype was significantly higher than GG genotype in body weight, breast muscle weight and leg muscle weight (P<0.05). At 24 weeks old,AA genotype was extremely significantly higher in body weight, carcass weight and semi-eviscerated weight than GG genotype (P<0.01),and AA genotype was significantly higher in eviscerated weight and leg muscle weight than GG genotype (P<0.05). GA genotype was significantly higher in body weight, carcass weight,semi-eviscerated weight and leg muscle weight than GG genotype (P<0.05), there were no significant difference in the other indexes among different genotypes at different ages (P>0.05). MSTN gene polymorphism was closely related to the production performance indexes of Baicheng fatty chicken, it was speculated this gene could be used as one of the molecular genetic markers for the breeding of Baicheng fatty chicken.

Key words: Baicheng fatty chicken; MSTN gene; polymorphism; production performance

CLC Number:

S813.3

WANG Yan-qin, SHAO Yong-gang. Study on the Association Relationship Between the Polymorphism of MSTN Gene and Production Performance in Baicheng Fatty Chicken[J]. , 2017, 44(4): 1054-1060.

References

[1] 郭振刚,张立春,曹阳,等.中国草原红牛肌肉生长抑制素基因3'-UTR多态性及其与屠宰性状的关联性分析[J].中国畜牧兽医,2013,40(10):184-187.
[2] 闵令江,丰艳妮,李兰,等.山羊MSTN基因多态性与主要经济性状的关联分析[J].畜牧兽医学报,2015,46(9):1515-1524.
[3] 胡师金,张淑珍,袁泽湖,等.绵羊CLPG和MSTN基因多态性及其与生长性状的关联分析[J].中国畜牧兽医,2016,43(5):1285-1293.
[4] Wang J,Zhou H,Hu J,et al. Two single nucleotide polymorphisms in the promoter of the ovine myostatin gene (MSTN) and their effect on growth and carcass muscle traits in New Zealand Romney sheep[J]. Journal of Animal Breeding and Genetics,2016,16(3):84-85.
[5] Trukhachev V,Belyaev V,Kvochko A,et al. Myostatin gene (MSTN) polymorphism with a negative effect on meat productivity in Dzhalginsky Merino sheep breed[J]. Journal of Bio science and Biotechnology,2015,4(2):191-199.
[6] 刘晓琴,马喜山,唐中林,等.猪MSTN基因的多态性和生长性状关联分析[J].畜牧兽医学报,2013,44(7):1063-1069.
[7] Sternstein I,Reissmann M,Maj D,et al. A new single nucleotide polymorphism in the rabbit (Oryctolagus cuniculus) myostatin (MSTN) gene is associated with carcass composition traits[J]. Animal Genetics,2014,45(4):596-599.
[8] 卢俊清,黄苇,赵楠,等.北京鸭肌肉生长抑制素基因多态与屠体性状关系分析[J].中国畜牧兽医,2008,35(10):61-64.
[9] 任冰冰,王康环,蒋利,等. 草科鸡MSTN基因多态性与其生长性状的相关性[J]. 贵州农业科学,2014,42(6):14-19.
[10] 左斌,任冰冰,蒋小松,等. 泸宁鸡和米易鸡MSTN基因多态性及其与生长性状的关联性[J]. 贵州农业科学,2015,43(7):26-30.
[11] 唐永凯,李建林,俞菊华,等. 吉富罗非鱼MSTN基因结构及其多态性与生长性状的相关性[J]. 中国水产科学,2010,17(1):44-51.
[12] 朱媛媛,梁宏伟,李忠,等. 黄颡鱼MSTN基因多态性及其与生长性状的相关性分析[J]. 遗传,2012,34(1):72-78.
[13] 格明古丽·木哈台,王永,刘利林,等.拜城油鸡种质特性测定[J]. 中国家禽,2013,35(22):57-60.
[14] 杨宁.家禽生产学[M].第2版.北京:中国农业出版社,2010.
[15] Li H W,Xu H Y,Zhao C J,et al. A PCR amplification method without DNA extraction[J]. Electrophoresis,2011,32(3-4):394-397.
[16] Zhang G X,Zhao X H,Wang J Y,et al. Effect of an exon 1 mutation in the myostatin gene on the growth traits of the Bian chicken[J]. Animal Genetics,2011,43(4):458-459.
[17] Zhang G X,Ding F X,Wang J Y,et al.Polymorphism in exons of the myostatin gene and its relationship with body weight traits in the Bian chicken[J].Biochemical Genetics,2011,49(1-2):9-19.
[18] 温言涛,武子寅,赵振华,等.鸡MSTN基因基因多态性及其与屠体性状的关联分析[J].中国家禽,2012,34(16):29-32.
[19] 赵振华,黎寿丰,黄华云,等. MSTN基因突变对优质鸡肌肉生长的影响[J]. 安徽农业大学学报,2015,42(5):733-737.
[20] 张跟喜,丁馥香,赵秀华,等. 边鸡肌肉生长抑制素基因5'调控区的多态性与生长性状的关联分析[J]. 中国畜牧杂志,2011,47(21):12-13.
[21] 魏伍川,袁凤媚,冯耀荣.湛江三黄鸡MSTN基因位点与屠宰率的相关分析[J].广东农业科学,2012,39(11):153-154.
[22] 张跟喜,丁馥香,是燕萍,等. 肌肉生长抑制素基因(MSTN)外显子1的多态性及其与边鸡生长性状的关联分析[J].农业生物技术学报,2011,19(1):122-127.
[23] Paswan C,Bhattacharya T K,Nagaraj C S,et al. SNPs in minimal promoter of myostatin (GDF-8) gene and its association with body weight in broiler chicken[J]. Journal of Applied Animal Research,2014,42(3):304-309.
[24] Dushyanth K,Bhattacharya T K,Shukla R,et al. Gene expression and polymorphism of myostatin gene and its association with growth traits in chicken[J]. Animal Biotechnology,2016,27(4):269-277.

Study on the Association Relationship Between the Polymorphism of MSTN Gene and Production Performance in Baicheng Fatty Chicken

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