疆岳驴催乳素受体基因多态性及其与泌乳性状间的相关性分析

doi:10.16431/j.cnki.1671-7236.2017.01.025

Abstract

Abstract:

In this study, the single nucleotide polymorphism of prolactin receptor (PRLR) gene and its correlation with the lactation traits in Jiangyue donkeys was tested, and the molecular genetic marker of breeding Jiangyue donkeys was explored.120 individuals were analyzed association of PRLR gene polymorphism with lactation traits in Jiangyue donkeys. Genetic polymorphism of PRLR gene flanking region was detected by PCR-SSCP method. The results showed that there was 1 mutation in PRLR gene and 2 genotypes of AA and AB, and allele A was the dominant allele. The SNP g.29764584 C>G was identified of PRLR gene by sequencing, and it was a synonymous mutation, did not cause amino acid changes. Statistical results indicated that the SNP was significantly associated with average daily milk yield and protein percentage in Jiangyue donkey (P<0.05),there was no significant in the lactin percentage(P>0.05). The mutation might be as crucial DNA genetic marker for lactation performance selection in Jiangyue donkeys.

Key words: Jiangyue donkeys; PRLR gene; polymorphism; lactation traits

CLC Number:

S813.3

BI Lan-shu, XIAO Hai-xia, ZANG Chang-jiang, HE Mei-sheng, TUOHUTI·Ajide, PAREHATIJIANG·Wufuer, YU Xi, LIU Wu-jun. Study on the Relationship between the Polymorphisms of PRLR Gene and Lactation Traits in Jiangyue Donkeys[J]. , 2017, 44(1): 180-185.

References

[1] 田方,王银朝,陈静波,等.新疆驴产业发展的机遇与挑战[J]. 草食家畜,2014,1:15-18.
[2] 陆东林,张明,江俊峰.发展新疆驴乳产业的探讨[J].中国乳业,2011,6:14-17.
[3] Viitala S,Szyda J,Blott S,et al.The role of the bovine growth hormone receptor and prolactin receptor genes in milk,fat and protein production in Finnish Ayrshire dairy cattle[J].Genetics,2006,173(4):2151-2164.
[4] 赵强. 绵羊PRLR,PRLH和PRLHR基因的克隆及其在下丘脑-垂体-性腺中表达的研究[D].兰州:甘肃农业大学,2015.
[5] 李晓林. 湖羊PRL、PRLR基因与泌乳性状相关性分析[D].石河子:石河子大学,2015.
[6] 王慧. PRLR基因在西农萨能羊乳腺上皮细胞中的RNA干扰研究[D].杨凌:西北农林科技大学,2013.
[7] Zhang J,Lan L,Fang P,et al.Genetic variation of PRLR gene and association with milk performance traits in dairy cattle[J].Can J Anim Sci,2008,88(1):33-39.
[8] Khatib H,Huang W,Wang X,et al.Single gene and gene inter-action effects on fertilization and embryonic survival rates in cattle[J].Dairy Sci,2009,92(5):2238-2247.
[9] Huang W,Mikhail D,Bindrim A C,et al.Interactions of the bovine placental lactogen and prolactin receptor genes are associated with fertility traits in cattle[J].Animal,2009,3(12):1743-1745.
[10] 董飞. 奶山羊乳腺组织差异表达miRNA功能预测及miR-135a对PRLR基因靶向调控研究[D].泰安:山东农业大学,2014.
[11] Yao X K, Yu S J, Liu W J,et al. Comparative study on some chemical-physical indicators in colostrum of two mare breeds[J].Asian Journal of Animal and Veterinary Advances,2011,6(5):451-459.
[12] 于茜,臧长江,刘玲玲,等. 伊犁马催乳素受体基因的遗传多态性及其与产奶量的关联分析[J].中国畜牧兽医,2015,42(9):2453-2458.
[13] 萨姆布鲁克J,拉塞尔D W. 分子克隆实验指南[M]. 第3版. 北京:北京科学出版社,2002.
[14] Giesecke K,HamannH, Sieme H,et al. Evaluation of prolactin receptor (PRLR) as candidate gene for male fertility in hanoverian warmblood horses[J]. Reproduction in Domestic Animals,2010,45(5):e124-e130.
[15] 肖国亮,周小玲,陈根元,等. 疆岳驴泌乳特征和产乳性能分析[J]. 中国奶牛,2015,1:16-20.
[16] 陆东林,张明.新疆疆岳驴乳研究进展[J].中国乳品工业,2013,2:32-36.
[17] 张佳兰,昝林森,申光磊. 牛催乳素受体(PRLR)基因HinfⅠ多态性与奶牛生产性能相关性研究[J]. 畜牧兽医学报,2007,38(9):888-892.
[18] 郭彦斌,杨海玲,王慧. 奶牛催乳素(PRL)及其受体(PRLR)基因研究进展[J].山东农业大学学报(自然科学版),2009,3:469-472.
[19] 张芬芬,王长法,黄金明,等. 催乳素受体的研究进展[J]. 家畜生态学报,2009, 3:6-9.
[20] Zidi A,Serradilla J M,Jordana J,et al.Pleiotropic effects of the goat prolactin receptor genotype on milk fatty acid composition[J].Domest Anim Endocrinol,2010,39(2):85-89.
[21] 曾献存,陈韩英,贾斌,等. 中国美利奴羊PROP1和PRLR基因的表达分析[J]. 畜牧兽医学报, 2011,42(8):1057-1062.
[22] 侯金星. 奶山羊产奶性状候选基因的筛选及其多基因聚合效应的研究[D].杨凌:西北农林科技大学,2013.
[23] 热阳谷.伊犁马及其杂交马催乳素与催乳素受体基因多态性的初步研究[D].乌鲁木齐:新疆农业大学,2012.
[24] Xiong Q, Chai J, Li X F,et al. Two tag SNPs in the prolactin receptor gene are associated with growth and litter traits in Boer and Macheng Black crossbred goats[J]. Livestock Science,2016, 193:71-77.
[25] 孙瑞萍,王利心,朱广琴,等.PRLR基因外显子10多态性与西农萨能奶山羊产奶性能的相关分析[J].畜牧兽医学报,2008,39(12):1654-1660.

Study on the Relationship between the Polymorphisms of PRLR Gene and Lactation Traits in Jiangyue Donkeys

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	WANG Zeping, WANG Feifei, ZHANG Ruiming, FU Yanfeng, ZHAO Weimin, DAI Chaohui, CHENG Jinhua, LIAO Chao, LI Hui. Analysis on Polymorphism of SLA-1 Gene and Its Disease Resistance Potential in Suzi Pig [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1918-1927.
[2]	ZHANG Rui, MU Chunyu, TANG Qingping, BU Zhu, YANG Mingjun, XIA Aiping, YANG Xiaoming. Polymorphism of GnIH Gene and Its Correlation with Egg-laying Traits in Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1412-1421.
[3]	TAN Yuancheng, LUO Hualun, ZHANG Yiyu, YANG Suan, WANG Yingtong, WANG Chunyuan. Polymorphism of MyoG Gene and Its Association with Body Size and Meat Traits in White Feather King Pigeons [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(4): 1461-1471.
[4]	LIU Zelin, YANG Juan, XU Mingzhu, ZHANG Nian, XIONG Qi, LIU Jingbo, TAO Hu. Association Analysis Between Polymorphisms of ZBP1 Gene and Lambing Performance in Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 161-173.
[5]	LONG Anju, YANG Suan, AI Jinxin, WENG Jimei, ZHANG Yiyu, CAI Huifen, LUO Weixing. Polymorphism of MC4R Gene and Its Association with Growth Traits in Qianbei Ma Goats [J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(1): 198-209.
[6]	XU Haoqi, XU Jingyi, HU Lirong, ZHANG Fan, LUO Hanpeng, ZHANG Hailiang, SHI Rui, LI Xiang, LIU Lin, LIU Qiaoxiang, GUO Gang, WANG Yachun. Polymorphism of PIK3CB Gene and Its Association Analysis with Reproductive and Milk Production Traits in Chinese Holstein Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(9): 3438-3452.
[7]	DING Mengqin, HEIDAXI·Bahati, PENG Fengqiang, HU Yali, SHAERSHANBIEKE·Abudilida. Analysis of Polymorphisms of FSHR Gene and Its Association with Production Performance of Baicheng You Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(8): 3044-3053.
[8]	ZHOU Di, AO Ye, JIANG Guirong, ZHAO Zhonghai, LI Jun, YANG Rong, GONG Fei, CHEN Changxue, SHEN Yin, LI Hui. Polymorphism of MEF2C Gene Exon and Its Association Analysis with Weight and Body Size Traits in Qiandongnan Xiaoxiang Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(7): 2613-2621.
[9]	MA Yanru, YU Yongsheng, CAO Yang, QIN Lihong, ZHAO Yumin, WU Jian. Polymorphism of PDK4 Gene and Its Association with Meat Quality Traits in Chinese Steppe Red Cattle [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2186-2194.
[10]	CHAI Zhixin, WU Zhijuan, WANG Jikun, ZHANG Chengfu, ZHONG Jincheng, XIN Jinwei. Polymorphisms of CTGF and Gprin3 Genes and Their Association with Body Size Traits in Yaks [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(6): 2216-2227.
[11]	RUAN Yong, CHEN Xiang, DAI Lingang, HUANG Jiajin, AN Dongwei, ZOU Qishun. Correlation Analysis of VNN1 Gene Polymorphism and Lambing Number in F3 Generation Boza Goats [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(4): 1384-1392.
[12]	ZHANG Yonghao, XIAO Yi, GUO Lijun, Tuerxun·Abudoureheman, WANG Yutao. Genetic Polymorphism Analysis of MHC-DRB3 Gene Exon 2 in Ovis ammon polii and Five Domestic Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(3): 962-972.
[13]	LI Mingming, WU Qingwei, DENG Yuting, JIN Shengwei, HE Na, ZHANG Junxia. Correlation Analysis of BMPRⅡ Gene Polymorphism and Its Haplotype with Lambing Traits in Tibetan Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(2): 587-597.
[14]	WANG Yingtong, YANG Suan, TAN Yuancheng, WANG Chunyuan, ZHANG Yiyu. Polymorphism of SLC8A1 Gene and Its Effect on Eggshell Quality in Changshun Blue-eggshell Chickens [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(12): 4688-4696.
[15]	FENG Mian, SHANG Mingyu, BI Yazhen, HU Wenping, ZHANG Li. Polymorphisms of FUT8 Gene and Its Association Analysis with Growth Traits in Sheep [J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(10): 3838-3845.