环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析

doi:10.16431/j.cnki.1671-7236.2016.07.025

Abstract

Abstract:

The purpose of the study was to analyze the association between MUC13 gene polymorphisms and piglet diarrhea in Huanjiang miniature pigs.93 diarrhea piglets and 95 healthy controls were included in the study.The MUC13 gene polymorphisms were determined using high resolution melting (HRM),and the results of HRM were verified by direct sequencing.Mutations were compared between diarrhea piglets and healthy controls.SHEsis online platform was used to calculate the haplotypes.There were statistically significant association on G224A site showed significant difference between diarrhea piglets and healthy controls by allele frequencies (P < 0.05),and there were also statistically significant differences between the two groups by genotypes among GG,GA,GG+GA and AA (P < 0.05).But no statistically significant association was found between polymorphisms of A112G,T170C and G164C sites and piglet diarrhea risk (P > 0.05).However,haplotype Ⅹ (GTAC) in the distribution of diarrhea group was extremely significantly higher (P < 0.01).The present data revealed that MUC13 gene G224A site and the haplotypes Ⅹ (GTAC) were associated with reduce risk of piglet diarrhea.

Key words: Huanjiang miniature pigs; MUC13 gene; SNP; haplotypes; piglet diarrhea

CLC Number:

S813.3

LI Jun, XU Zhong-feng, ZHANG Hai-hang, SHANG Yue-li, KONG Xiang-feng, SHI De-shun, LIU Qing-you. Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs[J]. , 2016, 43(7): 1818-1825.

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Association Analysis of MUC13 Gene Polymorphisms with Diarrhea in Huanjiang Miniature Pigs

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