绵羊CLPG和MSTN基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2016.05.025

Abstract

Abstract: The study was conducted to explore the possibility that CLPG (Callipyge) and MSTN (Myostatin) genes which could be the candidate genes of sheep growth traits, and investigate the molecular genetic markers related to sheep growth traits.133 (Austrilian White sheep×Dorper sheep×Hu sheep) hybid-sheep were chosen as subjects, the technology of direct sequencing of PCR products and PCR-RFLP were used to detect the single nucleotide polymorphism of CLPG and MSTN genes, then the association of the SNPs different genotypes and combined genotypes with sheep growth traits were analyzed by the GLM statistical model of SPSS 22.0.Sequencing results showed that the SNP of C/T which called C1 was detected at position 232 bp of the STS sequence in CLPG gene.The SNP of G/A which called M1 was detected in the 3'UTR of MSTN gene.PCR-RFLP analysis showed that two genotypes CC and CT were in C1 site, two genotypes GG and GA were in M1 site.Association analysis revealed that C1 site was significantly or extremely significantly associated with backfat thickness and loin muscle area (P<0.05;P<0.01), M1 site was significantly or extremely significantly associated with body weight, tube girth, backfat thickness and loin muscle area (P<0.05;P<0.01).Meanwhile, the combined genotype was extremely significantly associated with body weight, backfat thickness and loin muscle area (P<0.01).The conclusions indicated that SNPs and combined genotype of CLPG and MSTN genes had effects on growth traits in sheep.C1 and M1 sites could be considered as effective genetic markers for sheep growth traits.

Key words: CLPG gene; MSTN gene; polymorphism; growth traits; association analysis

CLC Number:

S813.3

HU Shi-jin, ZHANG Shu-zhen, YUAN Ze-hu, XUAN Jun-li, MA Xiao-meng, ZHANG Li, ZHAO Fu-ping, WEI Cai-hong, DU Li-xin. Polymorphism of CLPG and MSTN Genes and their Association Analysis with Growth Traits in Sheep[J]. , 2016, 43(5): 1285-1293.

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Polymorphism of CLPG and MSTN Genes and their Association Analysis with Growth Traits in Sheep

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