天祝白牦牛和甘南牦牛DRB3基因外显子2多态性研究

doi:10.16431/j.cnki.1671-7236.2016.03.027

Abstract

Abstract: In this study,CDS sequence of DRB3 gene exon 2 in Tianzhu White yak and Gannan yak were analyzed,a clean single chain was obtained to get the accurate haplotype by direct sequencing and amplification refractory mutation system (ARMS) method,the double chain of high heterozygosity was typed.The results showed that 65 SNPs and a codon insertion and deletion were discovered,all together separated to 30 haplotypes,in which seven haplotypes were found firstly,it should be peculiar to Tianzhu White yak and Gannan yak.Compared with published bovine DRB3 gene exon 2 haplotypes,the analysis results showed Tianzhu White yak and Gannan yak shared haplotypes of cattle (Bos taurus) and zebu (Bos indicus),which meaned they might crossed with cattle (Bos taurus) and zebu (Bos indicus).PAML analysis showed that 4,25,30,53,59,62,63,66,78 amino acid sites were affected by positive selection,and mainly occurred in Tianzhu yak,it had a certain relation with breeding history of Tianzhu White yak.

Key words: Tianzhu White yak; Gannan yak; DRB3 gene; exon 2; polymorphism

CLC Number:

S813.3

SHA Ri-nai, AN Tian-wu, E Guang-xin, HAN Jian-lin. Polymorphism Study on DRB3 Gene Exon 2 in Tianzhu White Yak and Gannan Yak Arlud[J]. , 2016, 43(3): 746-753.

References

[1] Birnbaum M E,Mendoza J L,Sethi D K,et al.Deconstructing the peptide-MHC specificity of T cell recognition[J].Cell,2014,157(5):1073-1087.
[2] 亢孝珍,额尔敦木图,姜建强,等.主要组织相容性复合体(MHC)基因研究进展[J].中国畜牧兽医,2014,41(5):28-33.
[3] Rojana-Udomsart A,Mitrpant C,James I,et al.Analysis of HLA-DRB3 alleles and supertypical genotypes in the MHC Class II region in sporadic inclusion body myositis[J].Journal of Neuroimmunology,2013,254(1-2):174-177.
[4] Osborne A J,John P,Negro S S,et al.Heterozygote advantage at MHC DRB may influence response to infectious disease epizootics[J].Molecular Ecology,2015,24(7):1419-1432.
[5] Takahata N,Nei M.Allelic genealogy under overdominant and frequency-dependent selection and polymorphism of major histocompatibility complex loci[J].Genetics,1990,124(124):967-978.
[6] Lewin H A,Russell G C,Glass E J.Comparative organization and function of the major histocompatibility complex of domesticated cattle[J].Immunological Reviews,1999,167(1):145-158.
[7] Miretti M M,Ferro J A,Lara M A,et al.Restriction fragment length polymorphism (RFLP) in exon 2 of the BoLA-DRB3 gene in South American cattle[J].Biochemical Genetics,2001,39(9-10):311-324.
[8] Newton C,Graham A,Heptinstall L,et al.Analysis of any point mutation in DNA.The amplification refractory mutation system (ARMS)[J].Nucleic Acids Research,1989,17(7):2503-2516.
[9] Joda H,Beni V,Katakis I,et al.DNA biosensor based on hybridization refractory mutation system approach for single mismatch detection[J].Anal Biochem,2015,474:66-68.
[10] Tamura K,Peterson D,Peterson N,et al.Mega 5:Molecular evolutionary genetics analysis using maximum likelihood,evolutionary distance,and maximum parsimony methods[J].Mol Biol Evol,2011,28(10):2731-2739.
[11] Librado P,Rozas J.DnaSP v5:A software for comprehensive analysis of DNA polymorphism data[J].Bioinformatics,2009,25(11):1451-1452.
[12] Yang Z.PAML 4:Phylogenetic analysis by maximum likelihood[J].Mol Biol Evol,2007,24(8):1586-1591.
[13] 包鹏甲,梁春年,郭宪,等.大通牦牛DRB3外显子2基因的遗传多样性[J].江苏农业学报,2013,29(4):800-806.
[14] Gelhaus A,Schnittger L,Mehlitz D,et al.Sequence and PCR-RFLP analysis of 14 novel BoLA-DRB3 alleles[J].Animal Genetics,1995,26(3):147-153.
[15] Russell G,Smith J,Oliver R.Structure of the BoLA-DRB3 gene and promoter[J].European Journal of Immunogenetics,2004,31(3):145-151.
[16] Maillard J C,Renard C,Chardon P,et al.Characterization of 18 new BoLA-DRB3 alleles[J].Animal Genetics,1999,30(3):200-203.
[17] Wiener G,Jianlin H,Ruijun L.The Yak[M].FAO Regional Office for Asia and the Pacific,2003.
[18] 梁育林.天祝白牦牛保种选育相关的问题与探讨[J].中国牛业科学,2008,34(4):73-75.
[19] 孙海涛.甘南牦牛产业化发展的制约因素与对策[J].中国牛业科学,2011,37(5):54-56.

Polymorphism Study on DRB3 Gene Exon 2 in Tianzhu White Yak and Gannan Yak Arlud

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