海南五指山猪Myf5与MyoD基因多态性分析

doi:10.16431/j.cnki.1671-7236.2015.06.030

Abstract

Abstract: This study was aimed to investigate the distribution of genetic polymorphisms of Myf5 and MyoD genes in Hainan Wuzhishan pig.PCR-RFLP method was used to detect the Myf5 and MyoD of 80 Wushishan pigs.Some characters of Myf5 and MyoD genes were analyzed by bioinformatics tools.The main results were as follows at the HhaⅠ-RFLP locus of exon 1 in Myf5 gene,CC and CG genetypes were major in Wuzhishan pig.At the Hsp92Ⅱ-RFLP locus of exon 1 in Myf5 gene,AA genetype was major in Wuzhishan pig.At the DdeⅠ-RFLP locus of intron 1 in MyoD gene,allele A was predominant in Wuzhishan pig.In addition,bioinformatics analysis revealed that Myf5 and MyoD genes promoter and exon 1 region contained CpG islands.In this study,we obtained the sequences of Myf5 and MyoD genes which laid a foundation for further studying their functions in Hainan Wuzhishan pig.

Key words: Wuzhishan pig; MyoD gene; Myf5 gene; polymorphism

CLC Number:

S813.3

WU Shu-jiang, CHAO Zhe. Polymorphism Analysis of Myf5 and MyoD Genes in Hainan Wuzhishan Pig[J]. , 2015, 42(6): 1524-1529.

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Polymorphism Analysis of Myf5 and MyoD Genes in Hainan Wuzhishan Pig

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