中国西门塔尔牛血管生成素样蛋白3基因多态性及其与肉质性状的关联性分析

doi:10.16431/j.cnki.1671-7236.2015.02.032

Abstract

Abstract: The aim of this study was to analyze polymorphism of angiopoietin-like protein 3 (ANGPTL3) gene and its association with meat quality traits in order to find markers in Chinese Simmental cattle. 98 Chinese Simmental cattles under identical feeding condition were selected randomly,the polymorphism of exon 4 of ANGPTL3 gene was analyzed by PCR-SSCP and direct sequencing methods.The association between different genotypes of exon 4 of ANGPTL3 gene and meat traits was analyzed in SPSS 19.0 program.One polymorphic site G7358C which had 2 genotypes of CC and CD was found in ANGPTL3 gene.Association analysis of polymorphisms of ANGPTL3 gene showed that the genotypes of different mutation in ANGPTL3 gene were significantly related to fat coverage of carcass,loin muscle area,marbling and IMF (P<0.05).The fat coverage of carcass,loin muscle area,marbling and IMF of individuals with CC genotype was significantly different from that of individuals with CD genotype (P<0.05).The results indicated that CC genotype was favorable genotype at this SNP locus and associated with fat coverage of carcass,loin muscle area,marbling and IMF,which suggested that ANGPTL3 gene could be studied as a candidate gene for molecular marker assisted selection.

Key words: ANGPTL3 gene; polymorphism; meat traits; Chinese Simmental cattle

CLC Number:

S813.3

HU Xin, WANG Guo-fu, WU Hui-guang, LI Chun, GAO Shu-xin. Polymorphism of ANGPTL3 Gene and its Association Analysis with Meat Traits in Chinese Simmental Cattle[J]. , 2015, 42(2): 438-442.

References

[1] Conklin D, Gilbertson D,Taft D W.Identification of a mammalian angiopoietin-related protein expressed specifically in liver[J].Genomics,1999,62(3):477-482.
[2] Guan Z B,Cai G L,Sun J Y, et al.Identification and expression analysis of bovine ANGPTL gene family[J].Current Zoology,2010,56(4):445-453.
[3] Feng S Q,Chen X D,Xia T,et al.Cloning,chromosome mapping and expression characteristics of porcine Angptl 3 and -4[J].Cytogenet Genome Res,2006,114(1):44-49.
[4] Kersten S,Lichtenstein L,Steenbergen E,et al.Caloric restriction and exercise increase plasma ANGPTL4 levels in humans via elevated free fatty acids[J].Arterioscler Thromb Vasc Biol,2009,29(6): 969-974.
[5] Yoon J C,Chickering T W,Rosen E D,et al.Peroxisome proliferator-activated receptor gamma target gene encoding a novel angiopoietin-related protein associated with adipose differentiation[J]. Mol Cell Biol,2000,20(14):5343-5349.
[6] Oike Y,Ito Y,Morisada T,et al.Angiopoietin-related growth factor (AGF) promotes angiogenesis[J]. Blood, 2004,103(10):3760-3765.
[7] Camenisch G,Pisabarro M T,Sherman D,et al.ANGPTL3 stimulates endothelial cell adhesion and migration via integrin alpha beta 3 and induces blood vessel formation in vivo[J].J Biol Chem,2002,277(19):17281-17290.
[8] Zheng J,Huynh H,Umikawa M,et al.Angiopoietin-like protein 3 supports the activity of hematopoietic stem stem cells in the bone marrow niche[J].Blood,2011,117(2):470-479.
[9] Leqry V,Bokor S,Cottel D,et al.Associations between commom genetic polymorphisms in angiopoietin-like protein 3 and 4 and lipid metabolism and adiposity in European adolescents and adults[J].J Clin Endocrinol Metab,2009,94(12):5070-5077.
[10] 杨婷.牛ANGPTL3基因组织表达、多态性及其与生长性状关联分析[D].郑州:河南农业大学,2012.
[11] 萨姆布鲁克J,拉塞尔D W.分子克隆实验指南(上、下册)[M].北京:科学出版社,2002.
[12] Nei M,Roychoudhury A K.Sampling variances of heterozygosity and genetic distance[J].Genetics,1974,76(2): 379-390.
[13] Nei M,Li W H.Mathematical model for studying genetic variation in terms of restriction endonucleases[J].Proceedings of the National Academy of Sciences of the United States of America,1979,76(10): 5269-5273.
[14] Botstein D,White R L,Skolnick M,et al.Construction of a genetic linkage map in man using restriction fragment length polymorphisms[J].American Journal of Human Genetics,1980,32(3): 314-331.
[15] Noto D,Cefalù A B,Valenti V,et al.Prevalence of ANGPTL3 and APOB gene mutations in subjects with combined hypolipidemia[J].Arteriosclerosis Thrombosis and Vascular Biology,2012,32(3):805-809.
[16] Martin-Campos J M,Roiq R,Mayoral C,et al.Identification of a novel mutation in the ANGPTL3 gene in two families diagnosed of familial hypobetalipoproteinemia without APOB mutation[J].Clinica Chimica Acta,2012,413(5-6):552-555.
[17] Hegele R A,Ban M R,Hsueh N,et al.A polygenic basis for four classical Fredrickson hyperlipoproteinemia phenotypes that are characterized by hypertriglyceridemia[J]. Human Molecular Genetics,2009,18(21):4189-4194.
[18] Teslovich T M,Musunuru K,Smith A V,et al.Biological,clinical and population relevance of 95 loci for blood lipids[J].Nature,2010,466(7307):707-713.
[19] Cline J L,Beckie T M.The relationships between FAM5C SNP (rs10920501) variability and metabolic syndrome and inflammation in women with coronary heart disease[J].Biol Res Nurs,2013,15(2):160-166.
[20] 陈宁博.牛能量代谢相关四个基因的遗传特征分析[D].信阳:信阳师范学院,2014.

Polymorphism of ANGPTL3 Gene and its Association Analysis with Meat Traits in Chinese Simmental Cattle

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