中国畜牧兽医

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陕西青脚麻肉鸡肠道定殖菌的生活状态和分布研究

王进1,侯天高2,赵娜娜1,陈文强1.3,邓百万1.3,韩世豪2,穆娜林4   

  1. 1.陕西理工学院生物科学与工程学院,陕西汉中  7230012.陕西省汉中中学,陕西汉中  7230003.陕西省食药用工程技术研究中心,陕西汉中  7230014.陕西大秦汉集团有限公司,陕西汉中  723000

  • 收稿日期:2013-11-25 出版日期:2014-05-20 发布日期:2014-06-25
  • 通讯作者: 陈文强(1956—),男,陕西人,教授,硕士生导师,研究方向:微生物资源的保护与利用。E-mail: wenqiangc@126.com
  • 作者简介:王进(1988—),男,陕西人,硕士生,研究方向:微生物的资源开发利用。
  • 基金资助:

    陕西省“春笋计划”2012-2013课题研究项目。

Study on Activities and Distribution of Bacterial Colonization in the Intestine of Shaanxi Qingjiaoma Chickens

WANG Jin1,HOU Tian-gao2,ZHAO Na-na1,CHEN Wen-qiang1.3,DENG Bai-wan1.3,HAN Shi-hao2,MU Na-lin4   

  1. 1.College of Biological Science and EngineeringShaanxi University of TechnologyHanzhong 723001,China2.Hanzhong High SchoolHanzhong 723000,China3.Shaanxi Engineering Research Center of Edible and Medicated FungiHanzhong 723001,China4.Shaanxi Han Group Co., Ltd.Hanzhong 723000,China

  • Received:2013-11-25 Online:2014-05-20 Published:2014-06-25

摘要: 为了解陕西青脚麻肉鸡肠道定殖菌的生活状态及分布,试验采用梯度稀释和组织培养方法对青脚麻肉鸡肠道定殖菌进行培养并计数。结果表明,芽孢菌在青脚麻肉鸡小肠中后段达到最大值,菌体总数达6.5×105 CFU/mL;酵母菌在盲肠中的菌体总数仅为6.8×104 CFU/mL;乳酸菌在十二指肠后段的菌体总数仅为8.6×104 CFU/mL。综上所述,陕西青脚麻肉鸡肠道中定殖有芽孢菌的分布,酵母菌和乳酸菌定殖甚少。

关键词:

益生菌; 肠道; 定殖菌; 陕西青脚麻肉鸡

Abstract: In the present study,we determined activities and distribution of bacterial colonization in the intestine of Shaanxi Qingjiaoma chickens.The microorganisms were cultivated and counted by gradient dilution and tissue culture method.The results showed that the middle and downside portions of small intestine contained Bacillus at a concentration of 6.5×105 CFU/mL. Saccharomyces was found in cesium at a concentration of 6.8×104 CFU/mL.Particularly,the downside portion of duodenum contained Lactobacillus at a concentration of 8.6×104 CFU/mL.Clearly,our results indicated that the Bacillus were rich in the intestine of Shaanxi Qingjiaoma chickens.In contrast,Saccharomyces and Lactobacillus as probiotics were less rich in the intestine.

Key words: probiotics; intestine; bacterial colonization; Shaanxi Qingjiaoma chickens