›› 2011, Vol. 38 ›› Issue (1): 126-128.

• 遗传繁育 • 上一篇    下一篇

MSTN基因外显子3的多态性及其与边鸡生长性状的关联分析

张跟喜1 ,丁馥香2 ,赵秀华1,王金玉1,金崇富1,张丽2   

  1. (1.扬州大学动物科学与技术学院,江苏扬州 225009; 2.山西省农业科学院畜牧兽医研究所,山西太原 030032)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-01-01 发布日期:2011-01-01
  • 通讯作者: 王金玉

Polymorphisms in Exon 5 of the Myostatin Gene and its Relationship with Growth Traits in Bian Chicken

ZHANG Gen-xi1,DING Fu-xiang2,ZHAO Xiu-hua1,WANG Jin-yu1,JIN Chong-fu1,ZHANG Li2   

  1. (1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China; 2. Institute of Animal Husbandry and Veterinary, Shanxi Academy of Agricultural Sciences, Taiyuan 030032, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-01-01 Published:2011-01-01
  • Contact: WANG Jin-yu

摘要:

采用PCR-SSCP技术检测边鸡及2个对照鸡群体(京海黄鸡和尤溪麻鸡)肌肉生长抑制素(myostatin, MSTN)基因外显子3的单核苷酸多态性,并与边鸡的生长性状进行相关性分析。结果表明,在MSTN基因外显子3的8527—8528 bp处检测到一个TC的插入。在边鸡和京海黄鸡中只检测到2种基因型(AA和AB基因型),在尤溪麻鸡中检测到3种基因型(AA、AB和BB基因型)。在3个鸡品种中都以A等位基因为优势等位基因。最小二乘分析结果表明,边鸡2种基因型个体的6~16周龄体重均无显著差异(P>0.05)。

关键词: 〗肌肉生长抑制素; 边鸡; PCR-SSCP; 生长性状

Abstract: Single nucleotide polymorphisms (SNPs) of exon 5 of the myostatin (MSTN) gene were detected in the Bian chicken and two reference chicken populations (Jinghai and Youxi chickens) by PCR-SSCP. The relationship with growth traits in the Bian chicken was also analysed. Results showed that there exsited an insretion (TC) in the 8527—8528 bp of the exon 5 of the MSTN gene. Three genotypes (AB, AB and BB) were detected in the Youxi chicken, while the Bian and Jinghai chickens only displayed two genotypes (AA and AB). The allele A was the predominant allele in the three chicken breeds. The least square means analysis showed the differences of body weight between the two genotypes in the Bian chicken were nonsignificant (P>0.05).

Key words: myostatin (MSTN); Bian chicken; PCR-SSCP; growth traits

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