江泉黑猪GPAT3基因多态性及其与生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2024.04.023

摘要/Abstract

摘要： 【目的】探究江泉黑猪甘油-3-磷酸酰基转移酶3(glycerol-3-phosphate acyltransferase 3,GPAT3)基因多态性及其与生长性状的相关性,为江泉黑猪的遗传改良和优化繁殖计划提供理论和技术支持。【方法】采集292头江泉黑猪的耳组织样并提取DNA,PCR扩增GPAT3基因片段,结合Sanger测序与MassARRAY^®核酸质谱分析系统检测单核苷酸多态性(single nucleotide polymorphism,SNP)位点及基因分型。利用R语言3.6.2、HaploView、PHASE等软件进行GPAT3基因SNP位点的遗传效应、连锁不平衡和单倍型分析。利用SAS 9.2软件分析GPAT3基因SNP位点及其构成的单倍型块与江泉黑猪生长性状的相关性。【结果】在GPAT3基因及其5'-调控区共发现10个SNPs,其中4个为低度多态,6个为中度多态;7个SNPs位点基因型频率分布处于哈代-温伯格平衡状态。关联分析发现,9个SNPs与江泉黑猪生长性状呈显著关联(P＜0.05),其中g.134957929 G＞A、g.134957930 G＞T、g.134958013 G＞A和g.134976983 T＞C存在极强连锁,构建的AGGT/AGGT双倍型在胸围、体高方面具有显著优势(P＜0.05),GTGC/GTGC双倍型和GTGC单倍型在体长方面具有显著优势(P＜0.05);g.135002556 C＞A、g.135002627 C＞T、g.135002680 C＞T存在极强连锁,构建的ATC/ATC双倍型在胸围、腹围方面具有显著优势(P＜0.05),CCC/ATC双倍型在背膘厚方面具有显著优势(P＜0.05),ATT单倍型在腹围方面具有显著优势(P<0.05)。【结论】江泉黑猪GPAT3基因具有丰富的多态性位点,9个SNPs及其构成的2个单倍型块与江泉黑猪胸围、腹围、体高等有显著关联性,可作为江泉黑猪生长性状选育的分子遗传标记。

关键词: 江泉黑猪; GPAT3基因; 单核苷酸多态性; 生长性状

Abstract: 【Objective】 The aim of this study was to investigate the polymorphism of glycerol-3-phosphate acyltransferase 3 (GPAT3) gene and its association with growth traits in Jiangquan Black pigs,so as to provide theoretical and technical support for genetic improvement and optimization of the breeding program in Jiangquan Black pigs.【Method】 The ear tissues of 292 Jiangquan Black pigs were collected and subjected to DNA extraction.The GPAT3 gene fragment was amplified using PCR,followed by detection of single nucleotide polymorphism (SNP) and genotyping through Sanger sequencing and MassARRAY^ nucleic acid mass spectrometry.The genetic effects,linkage disequilibrium and haplotype analysis of GPAT3 gene SNP were analyzed using R 3.6.2,HaploView and PHASE softwares.The association between GPAT3 gene SNP and its LD block with growth traits in Jiangquan Black pigs was assessed using SAS 9.2 software.【Result】 A total of 10 SNPs were found in GPAT3 gene and its 5'-regulatory region,of which 4 were low polymorphisms and 6 were moderate polymorphisms.The genotype frequency distribution of 7 SNPs was in Hardy-Weinberg equilibrium.Association analysis found that 9 SNPs were significantly associated with growth traits in Jiangquan Black pigs (P＜0.05),where g.134957929 G＞A,g.134957930 G＞T,g.134958013 G＞A and g.134976983 T＞C had a strong linkage,and the construction of AGGT/AGGT diplotype had significant advantages in chest circumference and body height (P＜0.05),GTGC/GTGC diplotype and GTGC haplotype had significant advantages in body length (P＜0.05).g.135002556 C＞ A,g.135002627 C＞T and g.135002680 C＞T had a strong linkage,and the construction of ATC/ATC diplotype had significant advantages in chest and abdominal circumference (P＜0.05),CCC/ATC diplotype had significant advantage in backfat thickness (P＜0.05),and ATT haplotype had significant advantage in abdominal circumference (P<0.05).【Conclusion】 There were abundant polymorphic loci of GPAT3 gene in Jiangquan Black pigs,9 SNPs and 2 haplotype blocks had significant correlation with the growth traits of chest circumference,abdominal circumference and body height,which could be used as molecular genetic markers for growth traits breeding in Jiangquan Black pigs.

Key words: Jiangquan Black pigs; GPAT3 gene; SNP; growth traits

中图分类号:

S813.3

刘汇鑫, 王文文, 许飞, 马小燕, 李晓敏, 唐辉. 江泉黑猪GPAT3基因多态性及其与生长性状的关联分析[J]. 中国畜牧兽医, 2024, 51(4): 1549-1560.

LIU Huixin, WANG Wenwen, XU Fei, MA Xiaoyan, LI Xiaomin, TANG Hui. Polymorphism of GPAT3 Gene and Its Association with Growth Traits in Jiangquan Black Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2024, 51(4): 1549-1560.

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