›› 2010, Vol. 37 ›› Issue (11): 105-109.

• 遗传繁育 • 上一篇    下一篇

鸡IGF-Ⅰ基因单核苷酸多态性及其与屠体性状关系的研究

靳薇1,张德祥2,鲁生霞2,戚小鸿2,王玳玮1,杨柳1,邓学梅1   

  1. (1.中国农业大学动物科技学院,农业部畜禽遗传育种重点实验室,北京 100193;2.广东温氏食品集团有限公司,新兴 527439)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-11-20 发布日期:2010-11-20
  • 通讯作者: 邓学梅

SNP of IGF-Ⅰ Gene and its Association with Carcass Traits in Chicken

JIN Wei1,ZHANG De-xiang2,LU Sheng-xia2,QI Xiao-hong2,WANG Dai-wei1,YANG Liu1,DENG Xue-mei1   

  1. (1.MOA Key Laboratory of Animal Genetics and Breeding,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;2.Guangdong Wenshi Food Co.Ltd.,Xinxing 527439,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-11-20 Published:2010-11-20
  • Contact: DENG Xue-mei

摘要: 本研究采用PCR-RFLP技术,对438只黄羽矮小型肉鸡的胰岛素样生长因子-Ⅰ基因(IGF-Ⅰ)的5′端进行遗传多态性研究,发现该区域的PCR扩增产物经Hinf Ⅰ酶切后出现GG、GT和TT 3种基因型。经χ2检验,群体在该位点上处于Hardy-Weinberg平衡状态(P>0.05)。方差分析结果显示,性别对9个性状(活体重、屠体重、皮脂厚、半净膛、全净膛、胸肌重、腿肌重、腹脂重、腹脂率)有极显著影响(P<0.01),对肌间脂肪含量有显著影响(P<0.05)。家系效应在8个性状上(活体重、屠体重、半净膛、全净膛、胸肌重、腿肌重、腹脂重、腹脂率)达到极显著水平(P<0.01);而基因型效应只在腹脂率上达到显著水平(P<0.05),腹脂重没有达到显著水平(P=0.0669)。不同基因型的最小二乘均值间多重比较结果发现,TT型的腹脂重和腹脂率均显著低于GT型(P<0.05),而GG型和GT、TT型的差异不显著(P>0.05)。

关键词: 鸡; IGF-Ⅰ基因; PCR-RFLP; 屠体性状

Abstract: In this study, the genetic polymorphism of insulin-like growth factor Ⅰ(IGF-Ⅰ) gene of 5′region in 438 yellow feather dwarf broilers was detected by PCR-RFLP method. PCR products treated by Hinf Ⅰ appeared GG,GT and TT genotypes. χ2 test showed the population was in Hardy-Weinberg equilibrium in this locus(P>0.05). Variance analysis indicated gender had extremely significant effect (P<0.01) on 9 traits (body weight and carcass weight,cutaneous fat thickness,half-eviscerated weight,eviscerated weight,breast muscle weight,drumstick weight,abdominal fate weight) and significant effect (P<0.05) on intramuscular fat,that family affected 8 traits extremely significantly (P<0.01),while that genotype effect was suggestively associated with abdominal fat rate (P<0.05),approximately suggestively associated with abdominal fat weight (P=0.0669). Multiple comparison of different genotypes’ least square mean displayed abdominal fat weight and abdominal fat rate of genotype TT were significantly lower than that of genotype GT (P<0.05),while that of genotype GG was no different significantly from genotype GT and TT (P>0.05)

Key words: chicken; insulin-like growth factor Ⅰ gene; PCR-RFLP; carcass trait

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