›› 2009, Vol. 36 ›› Issue (5): 116-119.

• 遗传繁育 • 上一篇    下一篇

安卡鸡Mx基因A2032G变异位点对经济性状的效应分析

刘艳1,栾德琴1,王克华2,常国斌1,包文斌1,陈国宏1   

  1. (1.扬州大学动物科学与技术学院, 扬州 225009; 2.中国农业科学院家禽研究所, 扬州 225003)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2009-05-20 发布日期:2009-05-20
  • 通讯作者: 陈国宏

The Genetic Effects of A2032G Mutation Point of Mx Gene on Some Commercial Traits in Anka Chicken

LIU Yan1, LUAN De-qin1, WANG Ke-hua2, CHANG Guo-bin1, BAO Wen-bin1, CHEN Guo-hong1   

  1. (1. Animal Science and Technology College, Yangzhou University, Yangzhou 225009, China;2. Institute of Poultry Science, Chinese Academy of Agriculture, Yangzhou 225003, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2009-05-20 Published:2009-05-20
  • Contact: CHEN Guo-hong

摘要: 采用错配PCR-RFLP对安卡鸡Mx基因2032位点多态性进行检测,结果表明,安卡鸡Mx基因2032位点经RsaⅠ酶切后,检测到AA、AG和GG 3种基因型,基因型频率分别为0.232、0.681和0.087,抗性等位基因A的频率为0.572。χ2适合性检验结果表明,安卡鸡Mx基因在RsaⅠ酶切位点上等位基因的分布显著偏离Hardy-Weinberg平衡状态。分析这3种基因型与安卡鸡一些经济性状间的关联性,结果表明,抗性纯合子AA基因型的个体各阶段体重、体尺、主要屠宰性状及常规肉质与AG和GG基因型的个体差异不显著,安卡鸡Mx基因2032位点对重要经济性状没有显著的负效应。

关键词: 鸡; Mx基因; 经济性状; 遗传效应

Abstract: The Mx gene in Anka chicken was analyzed with mismatch PCR-RFLP method, the results showed that M2032 locus of Mx gene digested by restriction endonuclease RsaⅠcould be divided into three kinds of genotypes, AA, GG and AG. The frequencies of these genotypes were 0.232, 0.681 and 0.087, respectively. The frequency of resistance allele A was 0.572. The result of Chi Square test indicated that the frequencies of Mx gene digested by RsaⅠ were significantly deviated from Hardy-Weinberg equilibrium in this study. The correlation between the three genotypes with some commercial traits was analyzed, the results showed that the body weight of different developmental stage, body size mainly slaughter traits and meat quality of the individuals were with resistant genotype AA were not significant different with those individuals with AG and GG genotypes in Anka chicken, which indicated that M2032 locus of Mx gene had no significantly negative genetic effects on commercial traits in Anka chicken.

Key words: chicken; Mx gene; commercial trait; genetic effect

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