›› 2008, Vol. 1 ›› Issue (7): 85-86.

• 疾病防治 • 上一篇    下一篇

鸡新城疫病毒与传染性支气管炎病毒同胚培养的试验研究

薛掌林,张述斌,刘瑞生,苟想珍,王必慧   

  1. 甘肃省畜牧兽医研究所, 平凉 744000
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2008-07-20 发布日期:2008-07-20

Homeomorphic Cultivation Research of Chicken Newcastle Disease Virus and Infectious Bronchitis Virus

XUE Zhang-lin, ZHANG Shu-bin, LIU Rui-sheng, GOU Xiang-zhen, WANG Bi-hui   

  1. Instiute of Gansu Veterinary and Animal Science, Pingliang 744000,China
  • Received:1900-01-01 Revised:1900-01-01 Online:2008-07-20 Published:2008-07-20

摘要: 将鸡新城疫病毒(NDV)和鸡传染性支气管炎病毒(IBV)按一定的稀释比例等量混合接种9~10日龄SPF鸡胚,让其在同一鸡胚中增殖(简称同胚培养)。试验结果表明,用5倍稀释的ND-Lasota病毒液与 5000倍稀释的IBH120病毒液等体积混合后尿囊腔接种鸡胚,能在同一鸡胚中正常增殖,接种后96 h收毒,用红细胞凝集试验(HA)测得NDV、IBV两种病毒的血凝价分别为11 log2和12 log2,不低于各自病毒单独增殖的HA滴度,病毒的增殖基本趋于平衡。同时也证实了在合适的培养条件下,IBV对NDV的复制不会产生干扰。

关键词: 鸡; 新城疫病毒; 传染性支气管炎病毒; 同胚培养; 血凝试验

Abstract: Chicken Newcastle disease virus(NDV) and infectious bronchitis virus(IBV)were diluted according to a certain proportion, then weve mixed equally, and the mixtures of the two virus were inoculated to 9~10 dog-old chicken embryos to research the proliferation of them in identical chicken embryos (abbreviating as homeomorphic cultivation). The results showed that: Inoculation of ND-Lasota strain (1∶5) and IBH120 strain (1∶5000) mixture, by allantoic cavity didnit influenced proliferation of the two virus in the identical chicken embryos. Hemagglutination titers of the two virus (NDV and IBV ) obtained after 96 h were respectively 11 log2 and 12 log2 by hemagglutination test, which were not lower than that of each one. Virus proliferation was tend to balance. Meanwhile, replication of NDV and IBV couldn’t produce interference effect each other under proper condition cultivation.

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