405 nm光谱协同二氧化钛的发光二极管灯源对蛋鸡生产性能、健康和舍内微生物的影响

doi:10.16431/j.cnki.1671-7236.2025.06.020

摘要/Abstract

摘要： 【目的】本试验旨在研究405 nm光谱协同二氧化钛(TiO₂)的发光二极管(LED)灯源应用于鸡舍对蛋鸡生产性能、健康及舍内环境微生物的影响。【方法】将480只产蛋率相近的39周龄京红1号蛋鸡随机分为2组，每组6个重复，每个重复40只蛋鸡，对照组使用常规LED灯，试验组使用405 nm光谱协同TiO₂的LED灯源，预试期1周，正式试验期12周。每日记录蛋鸡生产性能数据，试验期第72天时，分别检测蛋品质，并测定蛋鸡强直静止时间(tonic immobility，TI)、血液抗氧化指标、免疫指标以及H9、H7、H5亚型禽流感病毒、鸡新城疫病毒(NDV)、鸡产蛋下降综合征病毒(EDSV)的抗体滴度，同时检测舍内环境微生物含量。【结果】①试验组入舍母鸡产蛋数、饲养日产蛋数及产蛋率显著高于对照组(P＜0.05)，蛋品质在两组间无显著差异(P＞0.05)。②试验组蛋鸡的强直静止时间、血清抗氧化及免疫指标较对照组无显著差异(P＞0.05)，试验组蛋鸡H5亚型禽流感病毒抗体滴度显著高于对照组(P＜0.05)，H7、H9亚型禽流感病毒、NDV与EDSV抗体滴度则无显著差异(P＞0.05)。③试验组舍内空气微生物及物体表面微生物较对照组分别降低41.43%与34.49%，差异均显著(P＜0.05)。舍内化学制剂喷雾消毒前后，各时间点试验组舍内空气中微生物总数均显著低于对照组(P＜0.05)；且消毒12 h后试验组喷雾消毒的杀灭率较对照组增加8.41百分点，消毒后48 h舍内空气中微生物的恢复率较对照组降低5.34百分点。【结论】405 nm光谱协同TiO₂的LED灯源应用于蛋鸡舍，在不影响蛋鸡群健康的情况下，同时能够提高蛋鸡的生产性能，应用405 nm光谱协同TiO₂的LED灯源较常规LED灯能降低舍内41.43%的空气微生物与34.49%的物体表面微生物，增加常规喷雾消毒的效果并抑制微生物增长。

关键词: 405 nm光谱; TiO₂; 生产性能; 蛋品质; 环境微生物

Abstract: 【Objective】 This experiment aimed to investigate the effects of a 405 nm spectrally light-emitting diode (LED) light source combined with titanium dioxide (TiO₂) applied in hen houses on the production performance,health,and microbial content in the housing environment of laying hens.【Method】 A total of 480 39-week-old Jinghong No.1 laying hens with similar egg production rates were randomly divided into 2 groups,each with 6 replicates (40 hens per replicate).The control group used conventional LED lighting,while the experimental group used a 405 nm spectrally synergistic TiO₂-based LED light sources.The trial included a 1-week pre-trial period and a 12-week formal trial period.Daily production performance data were recorded.On day 72 of the trial,egg quality,tonic immobility (TI) duration,serum antioxidant and immune indices,antibody titers against Avian influenza virus H9,H7 and H5 subtypes,Newcastle disease virus (NDV),and Egg drop syndrome virus (EDSV),as well as microbial content in the housing environment,were measured.【Result】 ①The hen-housed egg production,hen-day egg production,and egg production rate of experimental group were significantly higher than those of control group (P＜0.05),while there was no significant differences in egg quality between the two groups (P＞0.05).②There was no significant difference in TI duration,serum antioxidant indices and immune indices between experimental and control groups (P＞0.05).The antibody titers against Avian influenza virus H5 subtype of hens in experimental group were significantly higher than those in control group (P＜0.05),while there was no significant difference in Avian influenza virus H7 and H9 subtypes,NDV,or EDSV antibody titers (P＞0.05).③ Compared to control group，the microbial content in the air and on surfaces in experimental group decreased by 41.43% and 34.49%,respectively (P＜0.05).Before and after chemical spray disinfection,the total number of microorganisms in the air of experimental group was significantly lower than that of control group at each time point (P＜0.05).The killing rate of spray disinfection in experimental group increased by 8.41 percentage points compared with control group at 12 hours after disinfection,and the recovery rate of microorganisms in the air in the house at 48 hours after disinfection decreased by 5.34 percentage points compared with control group.【Conclusion】 The application of a 405 nm spectrally synergistic TiO₂-based LED light sources in laying hen houses improved egg production performance without compromising flock health.Compared to conventional LED lighting,the use of the 405 nm spectrally synergistic TiO₂-based LED light sources reduced airborne microbial content by 41.43% and surface microbial content by 34.49% in the housing environment.Additionally,it enhanced the efficacy of routine spray disinfection and suppressed microbial regrowth.

Key words: 405 nm spectrum; TiO₂; production performance; egg quality; environmental microorganisms

中图分类号:

S831.4⁺5

姬逸凡, 石雷, 陈一凡, 刘喆, 袁文, 郑旭, 李艳慧, 马力峰, 苏丹, 叶仁良, 苏博斐, 陈辉. 405 nm光谱协同二氧化钛的发光二极管灯源对蛋鸡生产性能、健康和舍内微生物的影响[J]. 中国畜牧兽医, 2025, 52(6): 2675-2683.

JI Yifan, SHI Lei, CHEN Yifan, LIU Zhe, YUAN Wen, ZHENG Xu, LI Yanhui, MA Lifeng, SU Dan, YE Renliang, SU Bofei, CHEN Hui. Effects of 405 nm Spectrally Synergistic TiO₂-based LED Light Sources on Production Performance,Health and House Microbiology of Laying Hens[J]. China Animal Husbandry & Veterinary Medicine, 2025, 52(6): 2675-2683.

参考文献

[1] 杨芳，刘明，陈冠希，等.封闭式畜禽舍内微生物气溶胶的分布规律及其危害[J].国外畜牧学：猪与禽，2017，37(10):68-73.YANG F,LIU M,CHEN G X,et al.Distribution patterns and hazards of microbial aerosols in enclosed livestock and poultry houses[J].Animal Science Abroad (Pigs and Poultry),2017,37(10):68-73.(in Chinese)
[2] 高敏,贾瑞志,仇天雷,等.集约化养鸡场空气环境中生物气溶胶特点研究[J].农业环境科学学报,2015，34(4):787-794.GAO M,JIA R Z,QIU T L,et al.Characteristics of bioaerosols in air environment of confined poultry feeding operations[J].Journal of Agro-Environment Science,2015,34(4)：787-794.(in Chinese)
[3] 杨文浩.鸡舍环境气载微生物和PM2.5的检测及成分分析[D].泰安：山东农业大学，2016.YANG W H.Detection and analysis of components of airborne microbes and PM2.5 in poultry house[D].Tai’an:Shandong Agricultural University,2016.(in Chinese)
[4] BASKERVILLE A，HUMPHREY T J，FITZGEORGE R B,et al.Airborne infection of laying hens with Salmonella Enteritidis phage type 4[J].Veterinary Record,1992,130(18):395.
[5] MACLEAN M,MACGREGOR S J,ANDERSON J G,et al.Environmental decontamination of a hospital isolation room using high-intensity narrow-spectrum light[J].Journal of Hospital Infection,2010,76(3):247-251.
[6] MACLEAN M,MCKENZIE K,ANDERSON J G,et al.405 nm light technology for the inactivation of pathogens and its potential role for environmental disinfection and infection control[J].Journal of Hospital Infection,2014,88(1):1-11.
[7] BACHE S E,MACLEAN M,MACGREGOR S J,et al.Clinical studies of the high-intensity narrow-spectrum light environmental decontamination system (HINS-light EDS),for continuous disinfection in the burn unit inpatient and outpatient settings[J].Burns,2012,38(1):69-76.
[8] MEMARZADEH F,OLMSTED R N,BARTLEY J M.Applications of ultraviolet germicidal irradiation disinfection in health care facilities:Effective adjunct,but not stand-alone technology[J].American Journal of Infection Control,2010,38(5 Suppl 1):S13-24.
[9] 李宁,张伟,李贵贤,等.TiO₂光催化剂的研究进展[J].精细化工,2021，38(11):2181-2188.LI N,ZHANG W,LI G X,et al.Research progress of TiO₂ photocatalysts[J].Fine Chemicals,2021,38(11):2181-2188.(in Chinese)
[10] 王骜,仪修佶.TiO₂光催化应用综述[J].当代化工研究,2021,8:1-4.WANG A,YI X J.Review on application of TiO₂ photocatalysis[J].Modern Chemical Research,2021,8:1-4.(in Chinese)
[11] 王佳炜,王国桥,于添,等.碳基改性TiO₂去除水中典型污染物的试验[J].净水技术,2023,42(4):121-129.WANG J W,WANG G Q,YU T,et al.Experiment on modified carbon-doped TiO₂ for typical pollutants removal in aqueous solution[J].Water Purification Technology,2023,42(4):121-129.(in Chinese)
[12] 陈宽维,高玉时,王志跃,等.中华人民共和国农业行业标准家禽生产性能名词术语和度量统计方法[J].中国禽业导刊,2006，15:45-46.CHEN K W,GAO Y S,WANG Z Y,et al.Agricultural industry standards of the People’s Republic of China poultry production performance terminology and measurement statistical methods[J].Guide to Chinese Poultry,2006,15:45-46.(in Chinese)
[13] FOGELHOLM J,INKABI S,HGLAND A,et al.Genetical genomics of tonic immobility in the chicken[J].Genes,2019,10(5):341.
[14] ZHANG A Y,XIANG R,LEI C W，et al.Spatial variability and evaluation of airborne bacteria concentration in manure belt poultry houses[J].Poultry Science,2019，3:1202-1210.
[15] 中华人民共和国国家卫生健康委员会.现场消毒评价标准:WS/T 797—2022[S/OL].北京:中国标准出版社,2022,6-1.http://www.nhc.gov.cn/wjw/s9488/202202/6a329f9 dde8442b989b64a89fa2c96 d6.shtml.NATIONAL HEALTH COMMISSION OF THE PEOPLE’S REPUBLIC OF CHINA.Standard for evaluating the efficacy of disinfection on site:WS/T 797—2022[S/OL].Beijing:Standards Press of China,2022,6-1.http://www.nhc.gov.cn/wjw/s9488/202202/6a329f9 dde8442b989b64a89fa2c96 d6.shtml.(in Chinese)
[16] 戴景林.评空气微生物平板采样的奥梅梁斯基换算式[J].环境与健康杂志,1994,4:173-176.DAI J L.Evaluation of the omelchuk conversion formula for air microbiological plate sampling[J].Journal of Environment and Health,1994,4:173-176.(in Chinese)
[17] 张岩,刘萌,乔梦泽,等.不同LED光色对太行鸡生产性能、蛋品质以及营养物质消化率的影响[J].中国家禽,2022,44(9):66-73.ZHANG Y,LIU M,QIAO M Z,et al.Effects of different LED light colors on production performance,egg quality and nutrient digestibility of Taihang chicken[J].China Poultry,2022,44(9):66-73.(in Chinese)
[18] 泮进明,王小双,蒋劲松,等.家禽规模养殖LED光环境调控技术进展与趋势分析[J].农业机械学报,2013,44(9):225-235.PAN J M,WANG X S,JIANG J S,et al.Advancement and trend of LED light environment control technology for intensive poultry production[J].Transactions of the Chinese Society for Agricultural Machinery,2013,44(9):225-235.(in Chinese)
[19] 额尔敦木图,陈耀星,王子旭,等.单色光对产蛋鸡蛋品质的影响[J].中国畜牧杂志,2007,5:48-51.ERDEMTU,CHEN Y X,WANG Z X,et al.Effect of monochromatic light on egg quality of laying hens[J].Chinese Journal of Animal Science,2007,5:48-51.(in Chinese)
[20] MIN J K,HOSSAN M D S,NAZMA A,et al.Effect of monochromatic light on sexual maturity,production performance and egg quality of laying hens[J].Avian Biology Research,2012,5(2):69-74.
[21] 王首驿.蛋鸡生产后期LED光环境对产蛋性能、蛋壳质量及钙磷代谢的影响[D].杭州：浙江大学,2023.WANG S Y.Effect of LED light environment on egg production performance,eggshell quality and calcium and phosphorus metabolism in late production of laying hens[D].Hangzhou:Zhejiang University,2023.(in Chinese)
[22] GLADBACH A,GLADBACH D J,QUILLFELDT P.Variations in leucocyte profiles and plasma biochemistry are related to different aspects of parental investment in male and female Upland geese Chloephaga picta leucoptera[J].Comparative Biochemistry & Physiology Part A Molecular & Integrative Physiology,2010,156(2):269-277.
[23] KILGAS P,MÄND R,MÄGI M,et al.Hematological parameters in brood-rearing great tits in relation to habitat,multiple breeding and sex[J].Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2006,144(2):224-231.
[24] 刘杰.不同TI表型肉鸡血液生化指标的检测及对LPS刺激反应性的研究[D].南京：南京农业大学,2016.LIU J.Studies on blood bioparameters correlated with TI duration and stress responses to LPS stimulation in different TI phenotype of broilers[D].Nanjing:Nanjing Agricultural University,2016.(in Chinese)
[25] MESSINA G,BOSCO R,AMODEO D,et al.Safer school with near-UV technology:Novel applications for environmental hygiene[J].Journal of Environmental Health Science and Engineering,2023,21(1):157-165.
[26] 孙若阳,赵显一.二氧化钛光催化剂的制备及应用进展[J].当代化工,2023,52(1):202-208.SUN R Y,ZHAO X Y.Preparation and application progress of titanium dioxide photocatalysts[J].Contemporary Chemical Industry,2023,52(1):202-208.(in Chinese)
[27] TOMB R M,MACLEAN M,COIA J E,et al.Assessment of the potential for resistance to antimicrobial violet-blue light in Staphylococcus aureus[J].Antimicrobial Resistance & Infection Control,2017,6:100.
[28] XIONG P,HU J.Inactivation/reactivation of antibiotic-resistant bacteria by a novel UVA/LED/TiO₂system[J].Water Research,2013,47(13):4547-4555.
[29] RATHNASINGHE R,JANGRA S,MIORIN L,et al.Lighting a better future:The virucidal effects of 405 nm visible light on SARS-CoV-2 and Influenza A virus[J].Scientific Reports,2021,11(1):1-10.
[30] 郭世宜,沈星灿.纳米TiO₂光催化杀灭大肠杆菌的超微结构研究[J].电子显微学报,2004,2:107-111.GUO S Y,SHEN X C.Ultrastructural studies on the photocatalytical sterilization using titanium dioxide nanoparticles[J].Journal of Chinese Electron Microscopy Society,2004,2:107-111.(in Chinese)
[31] 张慧书,刘守新.TiO₂光催化杀菌机理及应用研究进展[J].科学技术与工程,2009,9(17):5049-5056.ZHANG H S,LIU S X.Progress of mechanism and application of photocatalytic bactericidal effect of TiO₂[J].Science Technology and Engineering,2009,9(17):5049-5056.(in Chinese)
[32] MANESS P,SMOLINSKI S,BLAKE D,et al.Bactericidal activity of photocatalytic TiO₂ reaction:Toward an understanding of its killing mechanism.[J].Applied & Environmental Microbiology,1999,65:4094-4098.
[33] MURDOCH L E,MICHELLE M,ENDARKO E,et al.Bactericidal effects of 405 nm light exposure demonstrated by inactivation of Escherichia,Salmonella, Shigella,Listeria,and Mycobacterium species in liquid suspensions and on exposed surfaces[J].The Scientific World Journal,2012;2012:137805.
[34] BUMAH V V,MASSON-MEYERS D S,CASHIN S,et al.Optimization of the antimicrobial effect of blue light on methicillin-resistant Staphylococcus aureus (MRSA) in vitro[J].Lasers in Surgery and Medicine,2015,47(3):266-272.
[35] 李佳辉,黄艺潇,马声,等.405 nm LED处理对不锈钢片表面金黄色葡萄球菌生物被膜的清除作用[J].现代食品科技,2022,38(1):63-72.LI J H,HUANG Y X,MA S,et al.Removal of Staphylococcus aureus biofilm on the surface of stainless steel by treating with 405 nm LED[J].Modern Food Science and Technology,2022,38(1):63-72.(in Chinese)
[36] 鹿院卫,马重芳,王伟,等.纳米光催化杀菌技术研究进展[J].北京工业大学学报,2006,7:622-627.LU Y W,MA C F,WANG W,et al.Study on the progress of nanometer photocatalytic disinfectant technology[J].Journal of Beijing University of Technology,2006,7:622-627.(in Chinese)
[37] SHANG C,BU J,SONG C.Preparation,antimicrobial properties under different light sources,mechanisms and applications of TiO₂:A review[J].Materials (Basel),2022,15(17):5820.