长白猪prepro-orexin基因多态性及其与采食和生长性状的关联分析

doi:10.16431/j.cnki.1671-7236.2018.03.020

《中国畜牧兽医》 ›› 2018, Vol. 45 ›› Issue (3): 712-720.doi: 10.16431/j.cnki.1671-7236.2018.03.020

长白猪prepro-orexin基因多态性及其与采食和生长性状的关联分析

胡伟¹, 李明丽¹, 张燕林², 严达伟¹, 李中昌², 董新星¹, 计春律², 兰国湘¹, 潘玉春³, 鲁绍雄¹

1. 云南农业大学动物科学技术学院, 昆明 650201;
2. 云南惠嘉育种有限公司, 昆明 650300;
3. 上海交通大学农业与生物学院, 上海 200240

收稿日期:2017-08-04 出版日期:2018-03-20 发布日期:2018-03-22
通讯作者: 潘玉春, 鲁绍雄 E-mail:panyc@sjtu.edu.cn;shxlu@263.net
作者简介:胡伟(1990-),男,安徽灵璧人,博士生,研究方向:遗传标记与动物育种,E-mail:huzhengwei2013@163.com
基金资助:
NSFC-云南联合基金项目（U1402266）；云南省高校科技创新团队支持计划（云教科[2014]22号）；云南省现代农业生猪产业技术体系建设项目（2017KJTX0013）

Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs

HU Wei¹, LI Mingli¹, ZHANG Yanlin², YAN Dawei¹, LI Zhongchang², DONG Xinxing¹, JI Chunlv², LAN Guoxiang¹, PAN Yuchun³, LU Shaoxiong¹

1. College of Animal Science and Technology, Yunnan Agricultural University, Kunming 650201, China;
2. Yunnan Huijia Breeding Co. Ltd., Kunming 650300, China;
3. School of Agriculture and Biology, Shanghai Jiaotong University, Shanghai 200240, China

Received:2017-08-04 Online:2018-03-20 Published:2018-03-22

摘要/Abstract

摘要：

为揭示食欲素前体（prepro-orexin）基因对长白猪采食和生长性状的影响，试验采用PCR产物直接测序法对132头长白猪prepro-orexin基因的C62T和G426A位点多态性进行检测，并对各位点与30~100 kg阶段的采食及生长性状进行关联分析。结果表明，长白猪prepro-orexin基因C62T和G426A位点均处于Hardy-Weinberg平衡状态，等位基因频率表现为野生型（C、G）高于突变型（T、A）；两个位点的4种单倍型和7种单倍型组合中，CG、TA为主要单倍型，CG/CG、TA/CG和TA/TA为主要单倍型组合。C62T位点的CC基因型、G426A位点的GG基因型及CG/CG单倍型组合均可显著减少日采食次数、延长每次采食时间和提高每次采食量（P < 0.05）；其中CG/CG组合的日采食次数、每次采食时间、每次采食量分别较TA/CG组合显著减少1.22次、延长0.79 min和提高32.18 g（P < 0.05），而与TA/TA组合的差异未达到显著水平（P > 0.05）。C62T位点的TT基因型、G426A位点的AA基因型及TA/TA单倍型组合可显著或极显著缩短达100 kg体重日龄、提高30~100 kg平均日增重（P < 0.05；P < 0.01）；TA/TA组合达100 kg体重日龄分别较CG/CG、TA/CG组合分别显著缩短6.23和6.69 d（P < 0.05），平均日增重分别极显著提高47.91和50.04 g/d（P < 0.01）。结果表明，prepro-orexin基因C62T和G426A位点对长白猪采食和生长性状的影响具有明显的协同效应。

关键词: 长白猪; 食欲素前体基因; 单核苷酸多态性; 采食性状; 生长性状

Abstract:

In order to investigate the effects of prepro-orexin gene on feeding and growth traits of Landrace pigs,the polymorphisms of C62T and G426A sites of prepro-orexin gene in 132 Landrace pigs were detected using PCR product direct sequencing,the association of the two sites with feeding and growth traits during 30 to 100 kg body weight were analyzed.The results showed that the two sites of prepro-orexin gene were in Hardy-Weinberg equilibrium in Landrace pigs,and the frequency of wild-type alleles C and G were higher than those of mutant-type alleles T and A;In the four haplotypes and seven haplotype combinations,CG,TA haplotypes and the combinations CG/CG,TA/CG,TA/TA were the dominant types.The CC genotype of C62T,GG genotype of G426A and haplotype combination CG/CG could significantly decrease the number of visits per day (NVD),prolong the time spent on eating per visit (TPV) and increase the feed intake per visit (FIV)(P < 0.05),which the NVD,TPV and FIV of combination CG/CG were 1.22 times fewer,0.79 min longer and 32.18 g greater than those of combination TA/CG (P < 0.05),but there were no significant difference from combination TA/TA (P > 0.05).The TT genotype of C62T,AA genotype of G426A and haplotype combination TA/TA could significantly or extremely significantly shorten the days to 100 kg body weight (D100) and increase the average daily gain (ADG) (P < 0.05 or P < 0.01),which the D100 and ADG of the combination TA/TA were 6.23 d and 47.91 g/d shorter than those of combination CG/CG (P < 0.05;P < 0.01),6.69 d and 50.04 g/d higher than those of combination TA/CG (P < 0.05;P < 0.01).In Landrace pigs,the influences of C62T and G426A sites of prepro-orexin gene on the feeding and growth traits had obvious synergistic effects.

Key words: Landrace pig; prepro-orexin gene; single nucleotide polymorphism (SNP); feeding trait; growth trait

中图分类号:

S813.3

胡伟, 李明丽, 张燕林, 严达伟, 李中昌, 董新星, 计春律, 兰国湘, 潘玉春, 鲁绍雄. 长白猪prepro-orexin基因多态性及其与采食和生长性状的关联分析[J]. 《中国畜牧兽医》, 2018, 45(3): 712-720.

HU Wei, LI Mingli, ZHANG Yanlin, YAN Dawei, LI Zhongchang, DONG Xinxing, JI Chunlv, LAN Guoxiang, PAN Yuchun, LU Shaoxiong. Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs[J]. , 2018, 45(3): 712-720.

参考文献

[1] DING R,QUAN J,YANG M,et al.Genome-wide association analysis reveals genetic loci and candidate genes for feeding behavior and eating efficiency in Duroc boars[J].PLoS One,2017,12(8):e0183244.
[2] COYNE J M,BERRY D P,MATILAINEN K,et al.Genetic co-variance functions for live weight,feed intake,and efficiency measures in growing pigs[J]. Journal of Animal Science,2017,95(9):3822-3832.
[3] 蒲蕾,刘欣,岳静伟,等.杜洛克猪HMGA1基因多态位点与生长、饲料利用性状的关联分析[J].中国畜牧兽医,2016,43(12):3268-3274. PU L,LIU X,YUE J W,et al.Study on HMGA1 gene polymorphism site and its association with feed efficiencyrelated traits in Duroc pigs[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3268-3274.(in Chinese)
[4] MORTON G J,CUMMINGS D E,BASKIN D G,et al.Central nervous system control of food intake and body weight[J].Nature,2006,443(7109):289-295.
[5] COTA D,PROULX K,SMITH K A B,et al.Hypothalamic mTOR signaling regulates food intake[J].Science,2006,312(5775):927-930.
[6] LENARD N R,BERTHOUD H R.Central and peripheral regulation of food intake and physical activity:Pathways and genes[J].Obesity,2008,16(S3):S11-S22.
[7] SAKURAI T,AMEMIYA A,ISHⅡ M,et al.Orexins and orexin receptors:A family of hypothalamic neuropeptides and G protein-coupled receptors that regulate feeding behavior[J].Cell,1998,92(4):573-585.
[8] DE LECEA L,KILDUFF T,PEYRON C,et al.The hypocretins:Hypothalamus-specific peptides with neuroexcitatory activity[J].Proceedings of the National Academy of Sciences,1998,95(1):322-327.
[9] TSUJINO N,SAKURAI T.Role of orexin in modulating arousal,feeding,and motivation[J].Frontiers in Behavioral Neuroscience,2013,7(28):1-14.
[10] SZÉKELY M,PÉTERVÁRI E,BALASKÓ M,et al.Effects of orexins on energy balance and thermoregulation[J].Regulatory Peptides,2002,104(1):47-53.
[11] WILLIE J T,CHEMELLI R M,SINTON C M,et al.To eat or to sleep? Orexin in the regulation of feeding and wakefulness[J].Annual Review of Neuroscience,2001,24(1):429-458.
[12] INUTSUKA A,INUI A,TABUCHI S,et al.Concurrent and robust regulation of feeding behaviors and metabolism by orexin neurons[J].Neuropharmacology,2014,85:451-460.
[13] KUNⅡ K,YAMANAKA A,NAMBU T,et al.Orexins/hypocretins regulate drinking behaviour[J].Brain Research,1999,842(1):256-261.
[14] SAKURAI T.The neural circuit of orexin (hypocretin):Maintaining sleep and wakefulness[J].Nature Reviews Neuroscience,2007,8(3):171-181.
[15] CHEN W,YE J,HAN D,et al.Association of prepro-orexin polymorphism with obstructive sleep apnea/hypopnea syndrome[J].American Journal of Otolaryngology,2012,33(1):31-36.
[16] KANTOR S,MOCHIZUKI T,LOPS S N,et al.Orexin gene therapy restores the timing and maintenance of wakefulness in narcoleptic mice[J].Sleep,2013,36(8):1129-1138.
[17] LIU M,BLANCO-CENTURION C,KONADHODE R R,et al.Orexin gene transfer into the amygdala suppresses both spontaneous and emotion-induced cataplexy in orexin-knockout mice[J].European Journal of Neuroscience,2016,43(5):681-688.
[18] TAMURA T,IRAHARA M,TEZUKA M,et al.Orexins,orexigenic hypothalamic neuropeptides,suppress the pulsatile secretion of luteinizing hormone in ovariectomized female rats[J].Biochemical and Biophysical Research Communications,1999,264(3):759-762.
[19] SMOLINSKA N,NITKIEWICZ A,MALESZKA A,et al.The effect of the estrous cycle on the expression of prepro-orexin gene and protein and the levels of orexin A and B in the porcine pituitary[J].Animal,2014,8(2):300-307.
[20] SMOLINSKA N,KIEZUN M,DOBRZYN K,et al.Adiponectin,orexin A and orexin B concentrations in the serum and uterine luminal fluid during early pregnancy of pigs[J].Animal Reproduction Science,2017,178:1-8.
[21] CHEN Q,LECEA L,HU Z,et al.The hypocretin/orexin system:An increasingly important role in neuropsychiatry[J].Medicinal Research Reviews,2015,35(1):152-197.
[22] MALEK M,MARKLUND S,DYER C,et al.Linkage and physical mapping of the porcine prepro-orexin gene[J].Mammalian Genome,2000,11(4):342-343.
[23] DYER C,TOUCHETTE K,CARROLL J,et al.Cloning of porcine prepro-orexin cDNA and effects of an intramuscular injection of synthetic porcine orexin-B on feed intake in young pigs[J].Domestic Animal Endocrinology,1999,16(3):145-148.
[24] 李靖.猪prepro-orexin基因多态性及其与生长繁殖性状相关性研究[D].南京:南京农业大学,2003. LI J.Ploymorphisms of prepro-orexin gene and their correlation with growth preformance and reproductivity in pigs[D].Nanjing:Nanjing Agricultural University,2003.(in Chinese)
[25] 管峰,罗媛媛,刘光富.Prepro-orexin基因多样性与猪产仔数的相关性[J].中国农学通报,2007,23(8):36-39. GUAN F,LUO Y Y,LIU G F.Polymorphism of pig prepro-orexin gene and its relationship on litter size[J].Chinese Agricultural Science Bulletin,2007,23(8):36-39.(in Chinese)
[26] 鲁绍雄,连林生.SAS统计分析系统在畜牧科学中的应用[M].昆明:云南科技出版社,2003. LU S X,LIAN L S.Application of Statistical Analysis System in Animal Science[M].Kunming:Yunnan Science and Technology Press, 2003.(in Chinese)

长白猪prepro-orexin基因多态性及其与采食和生长性状的关联分析

Polymorphism of prepro-orexin Gene and Its Association Analysis with Feeding and Growth Traits in Landrace Pigs

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